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Colletotrichum dematium was isolated from caraway for the first time in Poland in 2005. Isolations of this fungus were repeated in 2006 and 2007. The cultures of fungus were obtained from superficially disinfected leaves, root necks, roots, stems and umbels. The isolates were identified on culture media: PDA and malt agar with addition of pieces of caraway stems and on the base of macro and microscopic structures. Studies on the biotic effect between C. dematium and other species of phyllosphere fungi of caraway showed that the majority of the studied species limited the growth and development of C. dematium, but the size of the limiting effect was different. The species from Trichoderma and Gliocladium genera were the most effective against C. dematium, causing degeneration and lysis of hyphae and precluded the formation of the pathogen's acervuli and conidia. C. dematium in dual culture with E. purpurascens, A. radicina, S. sclerotiorum, B. cinerea and R. solani produced an inhibition zone which indicated its capacity for antibiosis.
Phomopsis diachenii was isolated from caraway cultivars Konczewicki, firstly in 2006 and next in 2007. Single cultures were obtained from the roots and the stem base of eight six-week-old seedlings and from the stems of two plants with symptoms of necrosis, in the second year of planting. This fungus was isolated from the plant parts superficially disinfected on malt agar medium with an addition 0.01% of streptomycin. The identification of the species was made on PDA medium. The biotic interactions between P. diachenii and S. carvi and other species of phyllosphere fungi of caraway were studied. Interactions among the fungi, i.e. between P. diachenii and one of the fungi representing the studied community, were examined using the biotic series method. The biotic effects of the fungi in dual cultures were evaluated after 10 and 20 days of common growth and were expressed as the individual biotic effect (IBE). It was shown that P. diachenii is a weak competitor because its growth was limited by numerous species of phyllosphere fungi. The obtained results indicated the dominance of biotic activity of P. diachenii over that of S. carvi. It is possible that P. diachenii has a greater ability to survive in the phyllosphere fungal community than S. carvi, causing septoriosis of caraway.
The aim of the study was to evaluate the resistance of caraway genotypes to water deficit based on the estimation of cell membrane stability (CMS) in leaves using polyethylene glycol (PEG) test. In 2007 and 2008, 25 selected caraway genotypes, originating from European botanical gardens (18), cultivars (2) and our own breeding strains (5), were tested. The plant material was collected from the experimental field. The obtained results showed highly significant differences in cell membrane injuries (p = 0.001) among investigated genotypes. The rank of genotypes in membrane injury index in 2007 was similar to that of 2008. Caraway genotypes originating from Warsaw (49.4%), Cracow (45.3%), Reykiavik (39.9%), Berlin (23.8%), Wisley (22.7%) and strains 9/2 (23.7%), 60/8 (22.2%) exhibited a high level of injury, which showed weak CMS and their high sensitivity to drought. The lowest extent of membrane injury was observed in genotypes originating from Bayreuth (4.2%), Ulm (4.4%), Cluj (5.5%), Lousanne (6.8%) and cultivar "Kończewicki" VI/4 (6.2%), which proves low sensitivity of these genotypes to water deficit and cell membrane stability. These genotypes may be used in further breeding program to improve caraway resistance to drought.
The study material consisted of K 1860 Septoria carvi isolate and single isolates of 18 other fungal species, chosen randomly from our own collection created during the studies of caraway diseases carried out in 2001-2006. Individual, general and summary biotic effects of the various fungi on Septoria carvi were determined by using the biotic series method and PDA (Difco) medium. Our results show that S. carvi is a weak competitor, because its growth was limited by all studied species of fungi representing the phyllosphere of caraway. Trichoderma, Gliocladium and Epicoccum purpurascens were recognized as positive and effective antagonistic fungi in relation to S. carvi. Moreover, the studied fungi that are pathogens of caraway and other plants, i.e. Fusarium spp., Rhizoctonia solani, Colletotrichum spp., Botrytis cinerea, Alternaria spp. and Phoma exigua proved strongly competitive to S. carvi, so these fungi could mask the symptoms of septoriosis at the first stage of the disease.
The aim of the study was to investigate the changes in the essential oil constituents of coriander and caraway when treated with UHP and/or elevated temperatures in a helium atmosphere. The UHP and/or heat treatment was conducted for 30 mins at 800 MPa, the temperature range was 60-121°C. The changes in the volatile oil content did not exceed 5% in all the treatment conditions. The pressure and temperature processing altered the composition of the essential oils of spices, which was examined using the GC-MS method. The most important sensory compounds, namely linalool in coriander oil and carvone in caraway oil, were not essentially changed after the processing. The final oil composition was different in the pressurised and not pressurised samples heated at the same temperature.
The aim of present work was to determine pathogenicity of three Colletotrichum dematium isolates to caraway. The effect of post-culture liquids and water suspension of conidia on germination of caraway schizocarps was studied in laboratory conditions. The effect of C. dematium on shooting up and healthiness of the seedlings was carried out in the climatic chamber using the method with infested soil and solidified plaster of culture medium overgrown by the mycelium of the pathogen. The studied isolates of fungus made the germination of parts of tested schizocarps impossible. On the other hand, all isolates caused necrosis of germs and roots of the other germinated schizocarps. The method with Colletotrichum dematium post-culture liquids was recognized as the best to a fast estimation of pathogenicity of fungus to caraway. Based on the positive results of pathogenicity tests the studied isolates of C. dematium were recognized as pathogenic to caraway. Considering this fact and repeated frequency of isolation of the fungus from various organs of this plant (M a c h o w i c z - S t e f a n i a k , 2010), C. dematium was recognized as a potentially pathogenic species to caraway.
Application of molecular markers makes the selection process much more effective. Marker assisted selection is an important tool for plant breeders to increase the efficiency of a breeding process, especially for multigenic traits, highly influenced by the environment. Epistasis is the interaction between alleles from two or more loci determining the complex traits, and thus plays an important role in the development of quantitative traits of crops. In this paper, the relationships between RAPD marker-by-marker interactions and 22 quantitative traits of caraway (Carum carvi L.) were analyzed. Significant associations of 116 epistatic markers with at least one trait in 2004 as well as 112 in 2005 were found on the basis of multivariate regression analysis. The proportion of total phenotypic variances of individual trait explained by the marker-by-marker interactions ranged from 25.3% to 96.0%.
The effects of caraway and peppermint extracts was evaluated at concentrations of 2, 4, 6, 8 and 10%, respectively on the radial mycelial growth of Sclerotium rolfsii. High significant inhibitory effect on radial fungal growth was observed with different concentrations of each of plant extracts. Concentration of 6% and more of the two extracts in combination were able to cause complete growth inhibition of the tested fungus. In greenhouse, the efficacy of plant extracts in addition to the fungicide Rizolex-T as seed dressing on pea root rot incidence was evaluated in pot experiment using soil artificially infested with the disease agent. All treatments showed a significant reduction in disease incidence compared with the control treatment. Rizolex-T followed by combination of caraway and peppermint extracts as a mixture showed superior reduction effect on root rot disease incidence at pre-, and post-emergence growth stages than individual treatment with each of extracts. The usage of caraway and peppermint extracts for seed dressing before sowing might be applied as control measure for controlling root rot diseases.
The aim of this study, which was conducted in field conditions on three localities in Serbia during two years, was to investigate the influence of the application of various types of fertilizers on the essential oil content in caraway, anise and coriander fruits. The influence of four organic fertilizers was investigated: two microbiological fertilizers (Slavol and Bactofil B-10), two specific organic fertilizers (Royal Ofert biohumus and vermicompost). Also, the chemical fertilizer was used and there was a control plot without any fertilization. From the results, it can be concluded that the application of different fertilizers has an influence on essential oil content only in case of anise fruits. The application of Royal Ofert biohumus shows the best results, after which follows chemical fertilizer. The use of these two types of fertilizers significantly increased the essential oil content in the anise fruits in comparison with other tested treatments. Although differences in the essential oil content of caraway and coriander were not significant, the two above mentioned fertilizers and vermicompost show the best results.
The macroscopic and microscopic characterization of fungus S. carvi and information on the occurrence of the pathogen on caraway are described. The cultures of 10 isolates were chosen randomly from the professional collection gathered during the studies of caraway diseases in 2001-2006. Identification of the pathogen was carried out on the basis of macroscopic and microscopic characterization in vivo and in vitro. The elements of fungi occurring on leaves, petioles, stems, umbels and schizocarps were studied in vivo. The cultures of fungus were studied on PDA medium at 24oC in dark conditions in vitro. Basing on macroscopic and microscopic characterization, the obtained isolates of the fungus were included in to Septoria carvi species. PDA medium was recognized as the best medium to cultivate and identification of S. carvi. The sizes of the fungus conidia obtained from PDA medium were approximate and comparable to those occurring on the host plant.
Oznaczono zawartość białka, tłuszczu, węglowodanów, popiołu oraz wody w składnikach codziennej diety takich jak mak, otręby, sezam, siemię lniane, płatki owsiane, kminek, orzechy włoskie i laskowe, soja, słonecznik oraz dynia. Z uzyskanych danych wynika, że produkty te dodawane do pieczywa, produktów zbożowych oraz cukierniczych wzbogacają nie tylko ich walory smakowe i estetyczne, ale przede wszystkim odżywcze.
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