Charakterystyka defektywnych szczepow MRSA nie wytwarzajacych koagulazy lub czynnika CF

• Autorzy: Garbacz K. , Piechowicz L. , Wisniewska K. , Galinski J.
• Języki publikacji: PL

Abstrakty

PL

Przebadano 186 szczepów MRSA pochodzących z różnych ośrodków regionu gdańskiego. Określono obecność genów nuc, mec A i coa metodą PCR. Wykryto 14 szczepów defektywnych, które nie wytwarzały koagulazy (7,25%) oraz 7 szczepów nie posiadających czynnika CF (3,76%). W celu scharakteryzowania szczepów defektywnych określono ich wzory restrykcyjne genu koagulazy (coa) metodą PCR-RFLP oraz wzór fagowy i lekooporność. Wzór restrykcyjny B był charakterystyczny dla koagulazoujemnych MRSA, podczas gdy wzór A był bardziej rozpowszechniony i występował zarówno wśród CF-ujemnych jak i CF-dodatnich MRSA.

EN

The aim of the study was to estimate frequency of coagulase-negative and CF-negative strains among methicillin-resistant Staphylococcus aureus (MRSA) and to assess their homogcnicity in respect of genotype, fagotype and drug resistance pattern. A total of 186 MRSA strains collected from different hospitals in Gdańsk region were studied. Gens: nuc, mecA, and coa were identified by PCR method. The coagulase tube test for staphylocoagulase and the slide test for clumping factor were used. Coagulase - negative and CF - negative MRSA strains were confirmed by PCR - RFLP method of coa gene; phage typing and drug resistance pattern were evaluated by disc diffusion test. The results of the study showed low frequency of both coagulase - negative and CF-negative MRSA strains (7,25% and 3,76% respectively). Among MRSA population tested the simultaneous occurrence of the strains lacking coagulase and clumping factor was not observed. All coagulase negative MRSA had coagulase gene (coa) and differed from CF-negative strains in respect of coa gene.

Słowa kluczowe

PL

lekoopornosc; gen coa; szczepy bakteryjne; mikrobiologia lekarska; gen mec A; koagulaza; lancuchowa reakcja polimerazy; zakazenia szpitalne; gen nuc; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2002
• Tom: 54
• Numer: 1
• Opis fizyczny: s.1-8,tab.,bibliogr.

Twórcy

autor

Garbacz K.

• Akademia Medyczna, Gdansk

autor

Piechowicz L.

autor

Wisniewska K.

autor

Galinski J.

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