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Staphylococcus aureus is a very important pathogen in humans about 20%of all bacterial infections are caused by S. aureus. Because the staphylococcal sensitivity patterns have changed, the aim of this study was to investigate the current susceptibility of the S. aureus strains to 9 antibiotics: penicilin (P), amoxacillin / clavulanic acid (AMC), erythromycin (E), cortimoxazole (SXT), tetracycline (T), chloramphenicol (C), mupirocin (MUP), gentamicin (Ge) and vancomycin (Va). Susceptibility testing was performed by disc diffusion technique, by the procedure outlined by the National Committe for Clinical Laboratory Standards (NCCLS). Susceptibility to methicillin was determined using 5 µm methicillin disks, ß-lactamase production in the penicillin - resistant strains was detected with nitrocefin impregnated disk (Cefinase, BBL Microbiology system). The microbiological characteristics of the samples: 338 of the isolates were derived, from noses and the throaths 382 - from surgical wound secretions. The analysis of the results showed that most S. aureus strains (82,8%) are penicillin resistant and ß-lactamase producing 13,5% of all strains were methicillin - resistant. 16,5% - were erythromycin resistant; 3,2% - were cotrimoxazolc resistant; 51,4% - were mupirocin resistant; 20,6% - were gentamicin resistant. All of the strains were vancomycin sensitive.
Przebadano wrażliwość na metycylinę 120 szczepów S. aureus wyhodowanych w laboratorium diagnostycznym Katedry i Zakładu Mikrobiologii Lekarskiej Akademii Medycznej w Warszawie z zastosowaniem dwóch metod: krążkowo-dyfuzyjnej i komercyjnej ATB STAPH S (wersja 2000). W przypadku 116 szczepów, co stanowiło ogółem 97% badanych szczepów, stwierdzono zgodne wyniki oznaczeń w obu metodach.
W latach 50-tych dowiedziono, że występujące w szpitalach szczepy Staphylococcus aureus różnią się od szczepów ze środowisk pozaszpi- talnych opornością na co najmniej kilka antybiotyków i przynależnością do I lub III grupy fagowej. W pracy porównano lekooporność i wzory fagowe 771 szczepów gronkowców złocistych wyizolowanych od pacjentów hospitalizowanych lub leczonych ambulatoryjnie. Dyskutowane jest zacieranie się różnic między szczepami S. aureus pochodzącymi z obu środowisk.
Przedstawiono wyniki badań, których analiza pozwoliła na zidentyfikowanie dwóch, wieloopornych, epidemicznych szczepów MRSA w Państwowym Szpitalu Klinicznym Nr 2 w Szczecinie.
The aim of this study was the investigation of adhesion of 88 S. aureus clinical isolates to collagen. The experiments were extended to determine the influence of growth temperature on collagen adhesin - collagen interaction. Bacterial adhesion to collagen was estimated by using immunoenzymatic assay at absorbance of 492 nm and compared with standard curves obtained for 8 different densities of each strain. The amount of collagen adhesin was indicated by colour reaction intensity measured by immunoenzymatic assay. Hydrophobicity of S. aureus strains was measured by aggregation in (NH4)2SO4 test. Almost all S. aureus strains isolated from bone and joint infections adhered to collagen whereas only a part of soft tissue infections isolates showed this feature. The comparison of adhesive properties of S. aureus cells cultured at 21ºC, 37°C and 42°'C did not make it possible to indicate the optimal culture temperature for S. aureus adhesion to collagen. However, the intensive colour reaction of cells cultured at 37°C with anti-collagen adhesin antibodies proves the production of the highest amount of this adhesin under the mentioned conditions. The influence of growth temperature as well as solid and/or liquid medium on the change of S. aureus hydrophobic properties was not observed. The obtained results show that the S- aureus growth temperature can be one of the factors influencing the staphylococci cells adhesion to collagen.
Wśród szczepów Staphylococcus aureus izolowanych w latach 1993 - 2001 poszukiwano szczepów VISA i hetero-VISA. Wykazano obecność takich szczepów. Zastosowano różne modyfikacje oryginalnej metody i analizowano ich przydatność.
Photodynamic therapy (PDT), used for cancer treatment, is also an alternative method for eradication of drug-resistant bacteria. This method utilizes a nontoxic light-activated dye, called a photosensitizer, and visible light to produce reactive oxygen species that lead to bacterial cell death. The purpose of this study was to investigate the bactericidal effect of PDT using lanthanide derivatives of meso-tetra(N-methyl-4-pyridyl)porphine against Staphylococcus aureusstrains. The new photosensitizers appeared to be photodynamically ineffective. No enhancement of antistaphylococcal activity of TMPyP was observed after the conjugation of the porphyrin with lanthanide ions. Additionally, a significant difference in the susceptibility of two bacterial strains to unmodified TMPyP was observed.
Cadmium is an environmental pollulant highly toxic to all forms of life. In this paper we studied by polarography the effect of the environment on cadmium speciation and also the effect of this speciation on 109Cd uptake and toxicity to living cells of cadmium-sensitive Staphylococcus aureus 17810S in different media. It was found that the magnitude of passive ,09Cd adsorption was proportional to the content of free Cd2+ in the medium (cadmium speciation). In contrast, the magnitude of energy-requiring l09Cd accumulation did not depend on cadmium speciation and was similar in all media. This could be due to the higher affinity of Cd2+ to the Mn2+ transport system than to the complexing ligands in the medium. Thus, cadmium uptake by S. aureus 17810S depends both on environmental and cellular factors and under all circumstances results in strong toxicity to living cells.
Bacteriostatic activity against Staphylococcus aureus of eleven single flavonoids, pinostrobin chalcone, cinnamic acid and four of its derivatives, hydrocinnamic acid, benzoic acid and six of its derivatives and vanillin occurring in propolis has been determined quantitatively by the method of serial dilutions. Moreover, the determination of bacteriostatic activity has been performed using ethanolic extract of propolis as well as that of three fractions obtained separately from propolis and identified earlier by GC-MS method. These were the following fractions: 1) the water fraction, 2) the fraction volatile with water vapour, 3) the flavonoids fraction. Bacteriostatic activities were also established for four flavonoids and eight organic acids using BIO method and the results of the used methods are compared and discussed. The highest bacteriostatic activity against Staphylococcus aureus displayed the following flavonoids: kaempferid, galangin, apigenin, quercetin, rhamnetin, pinocembrin and kaempherol.
A methicillin-resistant Staphylococcus aureus (MRSA) referred to as livestock-associated (LA-MRSA) has recently emerged in farm animals, particularly in pigs. Strains of this MRSA variant from Europe and North America mostly belong to clonal complex (CC) 398. Generally LA-MRSA cause asymptomatic colonization among pigs, but also in veal calves, broiler chickens, turkeys, and horses. People in contact with livestock animals are at high risk of asymptomatic colonization or infection with these bacteria. In previous years, the impact of LA-MRSA on human health was considered small. However, LA-MRSA has become more prevalent among people without direct livestock contact, especially in areas with a high density of pig production. As a result of horizontal gene transfer S. aureus CC398 strains are constantly evolving. The adaptive power of S. aureus to new hosts and acquisition of resistance to antibiotics may cause the emergence of new, more virulent clones. LA-MRSA has evolved from human-adapted methicillin-susceptible S. aureus CC398, which was proved by comparative genome analysis. The adaptation to livestock was associated with several genetic changes. The most worrying aspect of MRSA CC398 seems to be its ability to spread to humans. For this reason, continuous surveillance of further genetic changes is recommended.
Oceniono częstość występowania oraz lekowrażliwość drobnoustrojów najczęściej izolowanych z próbek materiału klinicznego pobranych od pacjentów Świętokrzyskiego Centrum Onkologii w Kielcach w 2001 roku, z uwzględnieniem podziału na rodzaje zakażeń. Najczęściej izolowano Escherichia coli i Staphylococcus aureus. Escherichia coli wytwarzające ESBL stanowiły 2,7% ogółu szczepów tego gatunku izolowanych z próbek materiału klinicznego pobranych od pacjentów hospitalizowanych, natomiast gronkowce złociste oporne na metycylinę 27,9% ogółu szczepów Staphylococcus aureus.
Wykazano znaczne różnice w częstości występowania wielolekoopornych szczepów Staphylococcus aureus w dwóch szpitalach warszawskich. Różnice te dotyczyły nie tylko odsetka szczepów MRSA, ale także szczepów MSSA o różnym spektrum lekooporności.
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