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The growing popularity of functional foods causes increasing interest in raw materials, which can raise the pro-health value of food when supplemented. The aim of the study was to assess the effect of compounds with antioxidant properties on Lactobacillus casei, as a representative of probiotic microorganisms. In the experiments the pure antioxidants and plant extracts were used. The results showed that catechin at concentration of 100-400 μM and chlorogenic acid (400 μM) had a stimulatory effect on L. casei growth. Quercetin at concentrations of 25-50 μM showed an inhibitory effect when incubation time was ≥6 h. The lingonberry leaves extract caused a slight increase in the dry substance of biomass in comparison with control. Extracts of coffee, chokeberry, and dog rose should not be used as additives for probiotic food as they significantly inhibited L. casei. We conclude that: 1) antioxidants at concentrations higher than 100 μM may stimulate growth of L. casei; 2) except the antioxidants, some other compounds which are present in plants extracts e.g. tannins and alkaloids may exert an antibacterial influence; 3) the probiotic yoghurts supplementation with fruit and other plant materials should be preceded by careful studies about their influence on the bacteria
Flow behaviour of yoghurts and bioyoghurts produced from goat’s unconcentrated milk at using different starter cultures was compared. Four starter cultures were applied to the production of yoghurts and four to the production of bioyoghurts too. Rheological properties were examined using rotary viscometer Rheotest 2 with controlled shear rate in coaxial cylinders system. Flow curves of yoghurts and bioyoghurts were assigned and described by Ostwald de Waele, Herschel-Bulkley and Casson models. An apparent viscosity was counted for shear rate 3 s-1. Generally, higher viscosity of yoghurts in comparison to bioyoghurts was stated. The yoghurts were generally characterised by higher value of consistency coefficient K and yield stress than bioyoghurts. The yoghurts have shown likewise higher deviation from Newtonian flow (lower values of exponent n) than bioyoghurts.
The research was aimed at comparing contents of cis9trans11 C18:2 acid (CLA) and trans isomers of C18:1 acid in commercial yoghurts and bioyoghurts as well as acquiring information whether CLA concentration in yoghurts and bioyoghurts differs from that in the initial material. Determinations were carried out with the method of gas chromatography using a 100-m capillary column with CP Sil 88 phase. Investigations demonstrated that in commercial yoghurts and bioyoghurts analysed the content of cis9trans11 C18:2 acid (CLA) and the total content of trans isomers of C18:1 acid were alike. The mean content of CLA in fat isolated from the yoghurts examined ranged from 0.41% to 0.43% of the total fatty acid composition, whereas in fat extracted from the bioyoghurts under study it ranged from 0.41% to 0.44%. The total content of trans isomers of C18:1 acid in yoghurts oscillated between 1.66% and 2.34% of the total fatty acid composition, and that in the bioyoghurts – between 1.95% and 2.91%. The content of cis9trans11 C18:2 acid in the total fatty acid composition of fat obtained from raw bulk milk examined did not differ from its content in fermented drinks produced from that milk. The mean content of CLA in fat of the yoghurts accounted for 0.53%, whereas that in fat of milk they were produced from – for 0.52%. Fat of the bioyoghurts analysed was characterised by a slightly lower content of cis9trans11 C18:2 acid as compared to the raw material. The mean content of trans isomers of C18:1 acid reached 2.25% in yoghurts, 2.00% in bioyoghurts and 2.13% of the total fatty acid composition in raw milk.
From ewe’s milk four fermented milk products were made (yoghurt, bioyoghurt, sour milk, kefir) using the following starter culture: for yoghurt: YC-180 (S. thermophilus and L. delbrueckii ssp. bulgaricus), bioyoghurt: ABT-1 (S. thermophilus, L. acidophilus and Bifidobacterium species), sour milk: CH-N-11 (L .lactis ssp. cremoris, L. lactis ssp. lactis, L. lactis ssp. diacetylactis, Leuconostoc mesenteroides ssp. cremoris) and for kefir: DA (Lactobacillus ssp., Lactococcus ssp. and yeasts). Raw milk, pasteurised milk, as well as all the products were analysed when fresh, after 7 and 14 day of storage for determination of vitamin C and ascorbic acid content, titratable acidity and pH.
The objective of the research was to determine the impact of the pasteurization process of raw material and of the type of packaging on the survival rate of probiotic bacteria in bio-yoghurts from goats’ milk during storage. The bio-yoghurts investigated were manufactured from goats’ milk using a container method. The milk under processing was centrifuged and normalized to a fat level of 2%. Next, it was pasteurised at a temperature of 95 °C during 5 min and at 90 °C during 10 min, after which it was cooled to 40 °C. The cooled milk was inoculated with DVS ABT1 inoculants added. The bioyoghurts were thermostated (controlled) in containers at a temperature of 40 °C (+/-1 °C) until they reached a pH level of 4.7. Then, they were cooled again to a temperature below 20 °C and poured into 4 different packagings made of polypropylene (PP), polystyrene (PS), polyethylene (PEHD), and glass (G), each of a capacity of 200 cm³. The bio-yoghurts were subsequently stored in the dark for 21 days at temperatures ranging from 2 to 5 °C. The presence of probiotic bacteria in bio-yoghurt was determined 12 h after the bio-yoghurts were manufactured, and on the 7th, 14th, and 21st day of storage. In total 240 samples were analysed. The applied packagings did not statistically significantly differentiated the count of bacteria in bio-yoghurts manufactured from goats’ milk, except for the Str. thermophilus bacteria count that changed only on the 14th day after manufacturing. The statistically significant impact of the pasteurisation temperature of raw material was found in the case of the L. acidophilus count immediately upon manufacturing. A lower pasteurisation temperature of goats’ milk had a favourable effect on the growth of bacteria, and in such bio-yoghurts the population of all the bacteria was higher. It was found that irrespective of the applied pasteurization temperature of raw material and of the type of packagings, the count of individual bacterial genera was at a level significantly higher than 10⁶ cfu/ml, which ensures that the milk drinks studied achieve the therapeutic minimum. Thus, expanding the production of goat milk bio-drinks can enhance the assortment of healthful diary products available in the market.
Background. Prebiotics are a category of nutritional compounds grouped together, not necessarily by structural similarities, but by ability to promote the growth of specific beneficial (probiotic) gut bacteria. Fructooligosaccharides (FOS) and inulin are among the most famous prebiotic compounds. In order to improve viability of probiotic bacteria during storage, fermented food should be supplemented with prebiotics. Material and methods. Yoghurts were produced from skimmed milk powder and prebiotics (FOS, inulin or resistant starch), which were added at concentrations of 1%, 2% and 3%. Yoghurts were stored in +4°C for three weeks. Every week each kind of fermented drink was examined in order to check the growth of lactic acid bacteria. Apparent viscosity and texture of bio-yoghurt were determinated during refrigerated storage. Results. The FOS and inulin addition to yoghurt caused an increase in the numbers of all bacteria in comparison to control yoghurt obtained without addition of prebiotics. The vi- able counts of Str. thermophilus, Lb. acidophilus and Bifidobacterium sp. when 1% of FOS was added to yoghurt were about 9 log cfu/g, 7.8 log cfu/g and 7.7 log cfu/g, respectively. In the presence of 1% of inulin, streptococci and bifidobacteria reached the growth at the level 8.8 log cfu/g and 7.5 respectively. Hardness and adhesiveness of yoghurt obtained with addition resistant starch increased systematically during 21 days of refrigerated storage. Conclusions. The numbers of lactic acid bacteria in obtained bio-yoghurts were sufficient in 97% of samples (106-109 cfu/g) according to FAO/WHO protocols. Generally, viability of bacteria was sufficient for 14 days and then their numbers decreased but usually not below 106 cfu/g. Prebiotics as FOS and inulin added to bio-yoghurt exhibited stimulatory effect on growth Lb. acidophilus and Bifidobacterium sp. Addition of prebiotics caused an increase in apparent viscosity and hardness (in case of FOS) and decrease in syneresis of obtained bio-yoghurts.
Celem pracy było zbadanie wpływu dodatku inuliny na cechy jakościowe jogurtów. W trzech seriach wyprodukowano przy użyciu szczepionki ABT1 trzy rodzaje jogurtu: naturalny jako próbę kontrolną i dwa z dodatkiem 1% i 3% inuliny. Badano cechy strukturalne, cechy organoleptyczne, kwasowość, pH, a także liczebność Str. thermophilus, L. Acidophilus oraz Bifidobacterium w produktach świeżych , przechowywanych tydzień i dwa tygodnie. Stwierdzono korzystny wpływ 1% dodatku inuliny na cechy organoleptyczne i teksturę jogurtu.
Badania preferencji i częstotliwości spożycia mleka i jego przetworów prowadzono w grupie 162 młodych kobiet, w tym 32 kobiet ciężarnych. Stwierdzono wysoką preferencję jogurtów i biojogurtów owocowych, twarożków owocowych i serka granulowanego oraz sera twardego. Najczęściej spożywanymi produktami były sery twarde, jogurty i biojogurty owocowe i twarożki z dodatkiem owoców. Stan ciąży wpływał na większe preferencje i spożycie napojów kwaśnych, jak maślanka i kefir oraz o dużej zawartości tłuszczu, jak mleko skondensowane. Korelacja między preferencją, a spożyciem była istotna w całej badanej grupie. Najbardziej ważkimi czynnikami wyboru były te związane z produktem: świeżość, smak, jakość, trwałość. Konieczność racjonalnego odżywiania, zawartość tłuszczu i nowość na rynku miały istotnie większe znaczenie dla kobiet ciężarnych, niż dla pozostałych.
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