Plant growth promoting rhizobacteria of cotton affecting the developmental stages of Helicoverpa armigera

• Autorzy: Vijayasamundeeswari A , Ladhalakshmi D. , Sankaralingam A. , Samiyappan R.

Warianty tytułu

PL

Wplyw bakterii korzeniowych stymulujacych wzrost roslin [PGPR] z bawelny na rozwoj Helicoverpa armigera

• Języki publikacji: EN

Abstrakty

EN

Rhizobacteria isolated from cotton roots exhibiting antagonism towards seedling blight and leaf blight of cotton were tested for their efficiency against the insect pest American bollworm (Helicoverpa armigera). The bioformulation developed using Bacillus subtilis (isolate DGL9) + chitin was found to be detrimental to the developmental stages of H. armigera (larva, pupa and adult) by causing larval mortality, pupal and adult malformation with reduced adult emergence. Generally, the larvae exhibited antifeeding behaviour when fed on bolls collected from rhizobacterial treatments. Hence, the developmental stages were altered leading to early pupation. Further, the efficacy of the isolate DGL9 was confirmed by culturing the bacteria in a suitable medium and incorporating the cell suspension and supernatant obtained form the broth culture in larval diet. The larvae fed to the diet exhibited defective developmental stages which was more significant in case of diet incorporated with supernatant. The percentage of pupal malformation, adult emergence and adult malformation was high at 96 h of incubation with the supernatant.

PL

Podczas badań testowano ryzobakterie wyizolowane z korzeni bawełny, które wykazują antagonizm w stosunku do zarazy rozsad i liści bawełny. Bakterie badano pod względem ich skuteczności przeciwko owadziemu szkodnikowi bawełny, amerykańskiej słonecznicy orężówce (Helicoverpa armigera). Biopreparaty sporządzone z użyciem Bacillus subtilis (izolat DGL9) + chityna okazały się szkodliwe dla stadiów rozwojowych Helicoverpa armigera (larwy, poczwarki oraz osobniki dorosłe) powodując śmiertelność larw, deformacje u poczwarek i dorosłych osobników oraz ograniczenie pojawiania się tych ostatnich. Ogólnie larwy wykazywały zachowanie antyfidantne w czasie żerowania na bawełnie traktowanej preparatem. Stadia rozwojowe ulegały zatem zmianom powodując przyspieszenie przepoczwarzania. Skuteczność izolatu DGL9 potwierdzono poprzez hodowlę bakterii w odpowiednim medium i wprowadzeniu do pożywienia larw zawiesiny komórek i supernatantu otrzymanego z bulionu z hodowli. Larwy żerowały na takim pożywieniu wykazując deformacje w stadiach rozwojowych, które były znaczniejsze w przypadku wprowadzenia supernatantu. Procent zdeformowanych poczwarek była wysoka po 96 godzinach inkubacji z supernatantem.

Słowa kluczowe

PL

cotton; rhizobacteria; plant growth; development stage; Helicoverpa armigera; Bacillus subtilis; Pseudomonas aeruginosa; root; seedling blight; leaf blight; insect pest; American boolworm

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2009
• Tom: 49
• Numer: 3
• Opis fizyczny: p.239-243,ref.

Twórcy

autor

Vijayasamundeeswari A

• Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India

autor

Ladhalakshmi D.

autor

Sankaralingam A.

autor

Samiyappan R.

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