Wyniki wyszukiwania - AGRO

1

Selected cellular mechanisms determining Pseudomonas aeruginosa biofilm formation under starvation conditions

100%

Myszka K., Czaczyk K.

Acta Scientiarum Polonorum. Biotechnologia

|

2012

|

tom 11

|

nr 4

2

Wlasciwosci hemaglutynacyjne szczepow Pseudomonas aeruginosa

100%

Wolska K., Jakubczak A.

Medycyna Weterynaryjna

|

2004

|

tom 60

|

nr 06

653-656

3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Increased production of carrageenase by Pseudomonas aeruginosa ZSL-2 using Taguchi experimental design

100%

Ziayoddin M., Lalitha J., Shinde M.

International Letters of Natural Sciences

|

2014

|

tom 12

|

nr 2

The culture conditions for the production of carrageenase were optimized using one-factor-at-a-time method combined with orthogonal array design. With the one-factor-at-a-time method revealed optimal conditions for carrageenase production were 24 h of fermentation period, 28 °C incubation temperature at pH 8.0 with NaNO3 as nitrogen source and carrageenan as carbon source in MMS media. Further optimization of carrgeenase production by using orthogonal experimental design L9 (34) with four factors, temperature, pH, NH4NO3 and carrageenan with their relevant levels revealed optimised conditions for carrageenase production were temperature of 28 °C, pH 8.0, 2 g L-1 NaNO3 and 2 g L-1 carrageenan. The order of the factors affecting the fermentation process was found to be temperature > pH > NaNO3 > carrageenan. The temperature played a significant role on the carrageenase production. Higher carrageenase yield with activity of 0.542 ±0.045 U ml-1 was obtained in the optimised medium when compared to those of basal medium. Carrageenase hydrolysed products of carrageenan were identified by LC-ESI-MS as neocarrabiose, neocarrabiose-4 sulfate, neocarratetraose, neocarratetraose-4 sulfate, anhydrogalactose, galactose, galactose-4 sulphate and sulphate.

4

Media optimization for depolymerization of alginate by Pseudomonas aeruginosa AG LSL-11

100%

Koti B. A., Manohar S., Lalitha J.

International Letters of Natural Sciences

|

2014

|

tom 14

An agar degrading bacterium Pseudomonas aeruginosa AG LSL-11 was acclimatized to alginate for the production of alginase. Production parameters such as pH, temperature, influence of simple carbohydrates and nitrogen sources, and effect of NaCl on growth and alginase production were carried out. Maximum growth was observed at pH 9.0 and 35 °C, while alginase was produced optimally at pH 9.0 and 30 °C. The alginase produced by Pseudomonas aeruginosa AG LSL-11 was inducible by alginate, and repressed by other simple sugar when supplemented along with alginate in the medium. The bacterium did not require NaCl for growth and production of alginase. The activity staining of partially purified culture supernatant after native PAGE revealed the presence of a single alginase.

5

Wplyw temperatury hodowli na hydrofobowe wlasciwosci paleczek Pseudomonas aeruginosa

100%

Wolska K., Bukowski K., Jakubczak A., Pogorzelska S.

Zeszyty Naukowe. Seria Rolnictwo. Akademia Podlaska

|

2001

|

tom 60

11-16

6

Production and characteristics of a heavy metals removing bioflocculant produced by Pseudomonas aeruginosa

100%

Gomaa E. Z.

Polish Journal of Microbiology

|

2012

|

tom 61

|

nr 4

The !occulating activity of a bioflocculant produced by Pseudomonas aeruginosa ATCC-10145 using kaolin clay was assayed. The in!uence of carbon, nitrogen sources, pH and culture temperature on bioflocculant production was investigated. The effects of cationic compounds, bioflocculant dosage, pH and temperature on flocculating activity were also determined. Of the cations tested, Ca2+, K +, Na+, Zn2+, Mg2+ and Cu2+ improved flocculating activity whereas Fe3+ and Al3+ caused its inhibition. The highest flocculating activity was observed at pH 7.0.The bioflocculant had a good flocculating activity of 80.50% for kaolin suspension with a dosage of only 1%. The bioflocculant was heat-stable and its activity was only decreased to 60.16% after heating at 100°C for 60 min. Chemical analyses of the purified bioflocculant indicated that it was a sugar-protein derivative, composed of protein (27%, w/w) and carbohydrate (89%,w/w ) including neutral sugar, uronic acid and amino sugar as the principal constituents in the relative weight proportions of 30.6%, 2.35% and 0.78%, respectively. The elemental analysis of the bio!occulant revealed the mass proportion of C, H and N was 19.06, 3.88 and 4.32 (%), correspondingly. Fourier transform infrared analysis showed that the exopolymers consisted of carboxyl, hydroxyl, amino and sugar derivative groups. The heavy metal adsorption by the bioflocculant of Pseudomonas aeruginosa was found to be influenced by the initial metal concentration, bioflocculant concentration and pH of the biosorption solution. This study demonstrates that microbial bioflocculant has potential to be used as an alternative bioremedial tool for industrial efluents and wastewater treatments which are co-contaminated with heavy metals.

7

Wrazliwosc szczepow Pseudomonas aeruginosa na wybrane chemioterapeutyki

88%

Wolska K., Bukowski K., Jakubczak A., Procka-Bal A.

Zeszyty Naukowe. Seria Rolnictwo. Akademia Podlaska

|

2001

|

tom 60

5-10

8

Hydrofobowe wlasciwosci szczepow Pseudomonas aeruginosa

88%

Jankowski S., Sarowska J., Zarczynska H., Cisowska A.

Medycyna Doświadczalna i Mikrobiologia

|

1997

|

tom 49

|

nr 3-4

187-190

Hydrophobic properties are considered as a factor enhancing the adhesion of bacteria to tissue cells. The strains of P. aeruginosa isolated from patients with urinary tract infections (UTI), from feces and soil were investigated. It shows that over 50% strains isolated from UTI had hydrophobic cell surface. Most of all strain investigated (67,9%) is characterized by hydrophobicity what probably favours their pathogenicity.

9

Typy serologiczne szczepow Pseudomonas aeruginosa wyizolowanych z roznych srodowisk

88%

Wolska K., Bukowski K.

Zeszyty Naukowe. Seria Rolnictwo. Akademia Podlaska

|

2002

|

tom 62

129-134

10

Aktywność przeciwbakteryjna olejku oreganowego (Origanum heracleoticum L.) wobec szczepów klinicznych Escherichia coli i Pseudomonas aeruginosa

75%

Sienkiewicz M., Wasiela M., Glowacka A.

Medycyna Doświadczalna i Mikrobiologia

|

2012

|

tom 64

|

nr 4

W niniejszej pracy zbadano aktywność przeciwbakteryjną olejku oreganowego (Origanum heracleoticum L) wobec 2 szczepów wzorcowych, 20 szczepów Escherichia coli oraz 20 szczepów Pseudomonas aeruginosa pochodzących z różnych materiałów klinicznych. Działanie olejku oreganowego oceniano metodą rozcieńczeń w podłożu agarowym, natomiast wrażliwość bakterii na antybiotyki badano metodą dyfuzyjno-krążkową. Na podstawie otrzymanych wyników stwierdzono, że testowany olejek oreganowy był aktywny wobec wszystkich testowanych szczepów klinicznych należących do obu rodzajów bakterii. Jednak szczepy Escherichia coli okazały bardziej wrażliwe. Olejek oreganowy pochodzący z Origanum heracleoticum L posiada właściwości hamujące wobec szczepów klinicznych należących do gatunków Escherichia coli oraz Pseudomonas aeruginosa o różnych wzorach oporności.

11

Pseudomonas aeruginosa w materiale diagnostycznym nadsylanym do ZHW w Warszawie

75%

Maciak T., Sawicka-Wrzosek K., Gosiewska A.

Życie Weterynaryjne

|

1996

|

tom 71

|

nr 04

116-119

12

Wplyw warunkow hodowli na hydrofobowe wlasciwosci paleczek Pseudomonas aeruginosa

75%

Wolska K., Pogorzelska S., Fijol E., Jakubczak A., Bukowski K.

Medycyna Doświadczalna i Mikrobiologia

|

2002

|

tom 54

|

nr 1

61-66

Hydrofobowe właściwości powierzchni komórki bakteryjnej (cell surface hydrophobicity, CSH) należą do najważniejszych nieswoistych czynników adhezji, która jest pierwszym etapem kolonizacji i zakażenia. W niniejszej pracy wykazano wpływ warunków hodowli, tj. rodzaju podłoża, czasu i temperatury inkubacji na zmianę hydrofobowych właściwości powierzchni komórek Pseudomonas aeruginosa.

13

Oznaczanie genu toxA determinujacego synteze toksyny letalnej u szczepow Pseudomonas aeruginosa wyosobnionych z nasienia knurow

75%

Czeczot-Urban A., Furowicz A. J.

Medycyna Weterynaryjna

|

2005

|

tom 61

|

nr 03

330-331

14

Powstawanie biofilmu Pseudomonas aeruginosa i jego znaczenie w patogenezie zakazen przewleklych

75%

Trafny E. A.

Postępy Mikrobiologii

|

2000

|

tom 39

|

nr 1

55-71

15

Kolonizacja ran pooperacyjnych endogennymi paleczkami Gram-ujemnych u pacjentow po chirurgicznym usunieciu raka krtani

75%

Rozalska M., Weber-Dabrowska B., Jozefowicz-Korczynska M.

Medycyna Doświadczalna i Mikrobiologia

|

2001

|

tom 53

|

nr 2

197-206

Porównano profile biochemiczne, wzory lekowrażliwości, typy bakteriocynowe i bakteriofagowe pałeczek Gram-ujemnych wyizolowanych z gardła, krtani oraz ran pooperacyjnych pacjentów leczonych chirurgicznie z powodu raka krtani. Wykazano kolonizację ran pooperacyjnych endogennymi pałeczkami Enterobacter cloacae i Pseudomonas aeruginosa.

16

Rola fimbrii w adherencji Pseudomonas aeruginosa do komorek nablonka policzkowego

75%

Wolska K., Kostrzewska M., Jakubczak A.

Zeszyty Naukowe. Seria Rolnictwo. Akademia Podlaska

|

2004

|

tom 65

113-120

17

Effect of adaptation process of Pseudomonas aeruginosa to didecyldimethylammonium chloride in 2-propanol on bactericidal efficiency of this active substance

75%

Chojecka A., Wiercinska O., Rohm-Rodowald E., Kanclerski K., Jakimiak B.

Roczniki Państwowego Zakładu Higieny

|

2014

|

tom 65

|

nr 4

Background. Microorganisms are characterized by two types of resistance innate and acquired. Innate resistance is associated with the construction of the surface structures. Wide use of active substances as antimicrobial compounds, especially in inhibitory concentrations, may promote the acquisition of bacterial resistance to these substances in the process of adaptation. Objective. The aim of the study was to determine changes in efficiency of didecyldimethylammonium chloride in 2-propanol (CMAP) against the Pseudomonas aeruginosa strains, which were adapted to this active substance. Materials and Method. Adaptation studies were conducted using two strains: P. aeruginosa ATCC 15442 (PA), which is used in estimation of biocide efficiency and tetracycline-resistant P. aeruginosa ATCC 47085 (PAO-LAC) strain. These strains were adapted to the active substance Bardac22: 50% v/v didecyldimethylammonium chloride in 20% v/v 2-propanol (CMAP) according to the National Institute of Hygiene procedure. After adaptation, obtained isolates were classified to three groups and passaged to solid media: A – strains unadapted passaged onto slant medium without active substance, control group; B – strains with adaptive resistance passaged onto slant medium with 375 mg/l CMAP; C – strains with adaptive resistance passaged onto slant medium without CMAP. Changes in susceptibility of examined strains were determined on the basis of minimum inhibitory concentrations (MICs) by broth dilution method. The minimum bactericidal concentrations (MBCs) were determined by subculture P. aeruginosa strains on solid media without CMAP. The efficiency of CMAP against isolates obtained after adaptation processes was evaluated by using phenol coefficient (PC). Results. There were no differences in the adaptation process between two strains of P. aeruginosa: PA and PAO-LAC. Both isolates obtained after the adaptation process was characterized by approximately 6-8 fold higher MICs compared to the MICs of these strains before the adaptation. Strains passaged to a solid media characterized a variable sensitivity to CMAP. As compared to a control group A, the isolates of PA and PAO-LAC from group B and isolate PA from group C exhibited the highest and stable insensitivity (MIC from > 700 to >1000 mg/l) to 48-49 passages. Isolates from group C of PAO-LAC maintained insusceptibility up to 20th passage (MIC >375 mg/l). There were no statistically significant changes in the CMAP bactericidal efficacy against isolates of reduced sensitivity. Conclusions. Adaptation of P. aeruginosa strains to didecyldimethylammonium chloride in 2-propanol does not significantly change bactericidal efficacy of this active substance against isolates with reduced sensitivity. Antibiotic-resistant strain PAO-LAC showed a similar adaptability and a similar sensitivity to the CMAP as a strain PA used to assess the effectiveness of disinfectants.

18

Environmental influence of cultural medium on bioherbicidal activities of Pseudomonas aeruginosa C1501 on mono and dico weeds

75%

Adetunji C. O., Oloke J. K., Prasad G., Akpor O. B.

Polish Journal of Natural Sciences

|

2017

|

tom 32

|

nr 4

Microbe producing natural herbicides are alternatives to the chemical herbicidal formulations. The effect of minerals and carbon sources were screened to select the best when combined and when apply singly during submerged fermentation. The effect of their phytotoxic metabolites was tested on Chromolaena odorata and Echinochola crus-galli. It was observed that the best combination between all the mineral was found in the combination containing manganese, zinc, bromine and iron. It gave the highest bio-herbicidal activities on the tested weeds when compared with the basal medium without any mineral amendment (P ≤ 0.05). The best carbon source screened was glucose while the best mineral screened was iron in term of showing activities on the tested weeds (P ≤ 0.05).

19

Biological degradation of metribuzin and profenofos by some efficient bacterial isolates

75%

Tamilselvan C., Joseph S. J., Mugunthan G., Sathish Kumar A., Syed Musthaq Ahamed S.

International Letters of Natural Sciences

|

2014

|

tom 09

The soil sample was collected from the paddy field of Sriperumbudur, Tamilnadu which is having a history of repeated pesticide applications. The isolation of efficient pesticide degrading bacteria was identified as Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. The growth of the three pesticide degrading isolates was assessed in Minimal salt broth containing 25 ppm of pesticides. Two popularly used pesticides Metribuzin and Profenofos were selected for this study. Among the three bacterial isolates, the bacteria Bacillus subtilis utilized the pesticides effectively and showed maximum growth. The growth of the three pesticides degrading isolates Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was assessed in Minimal salt broth containing 25 ppm of pesticides at different temperature levels (25 °C, 30 °C, 35 °C, 40 °C, 45 °C, 50 °C & 55 °C) and pH levels (pH 4, pH 5, pH 6, pH 7 & pH 8) and carbon sources (Lactose, Dextrose, Fructose, Mannose & Galactose) and nitrogen sources Peptone, Yeast extract, Beef extract, Malt extract and Casein respectively. The maximum growth rate of bacteria was recorded at 35 °C and pH 6. The maximum growth of bacteria was in the presence of Dextrose followed by Fructose, Galactose and Mannose. The least growth was recorded in Lactose broth culture. The maximum growth of bacteria was in the presence of Malt extract followed by Peptone, Yeast extract and Casein. The least growth was recorded in Beef extract broth culture. The bacterial isolates showed maximum growth in the Minimal salt broth containing Profenofos followed by Metribuzin.

20

Wstępne rozpoznanie występowania Pseudomonas aeruginosa w środkach spożywczych

75%

Burzynska H., Maciejska K., Borowiak M., Czarnowska W., Dziurowicz Z., Gorecka E., Grubner M., Juchnowicz I., Kasza I., Koc T., Lewicka J., Maciaszek A., Smykal B., Wilczynska-Stelmach W.

Roczniki Państwowego Zakładu Higieny

|

1974

|

tom 25

|

nr 6

Ograniczanie wyników