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Due to the importance of the biological control of plant diseases, testing and introducing new biocontrol-active microorganisms is a major concern among plant pathologists. The causal agent of cotton seedling damping-off disease is Rhizoctonia solani. In this regard, we tried to investigate the antagonistic activities of Pseudomonas aureofaciens (chlororaphis) 30–84 (phenazine producing wild type and non-phenazine producing mutant) strains on R. solani, in comparison with some isolates of P. fluorescent under both in vitro (laboratory) and in vivo (greenhouse) conditions. In the laboratory experiment, the inhibitory effects of all the bacteria, on the growth of R. solani, were evaluated using the dual culture procedure. Results showed that five isolates of P. fluorescent along with both strains of P. aureofaciens significantly inhibited the growth of R. solani. Effective bacterial antagonists were then evaluated in a greenhouse experiment where cotton seeds were coated with their suspensions and were sown in pasteurised field-soil. The soil had been pre-inoculated with a virulent isolate of R. solani. The efficacy of the bacterial antagonists was evaluated by counting the number of surviving seedlings in different treatments, at 15 and 60 days after sowing, for determining pre- and post-emergence damping-off incidence. According to the results of the greenhouse experiment, at both intervals, two isolates of P. fluorescens along with both strains of P. aureofaciens caused significant increases in the number of healthy seedlings, in comparison with the untreated control, and a commonly used fungicide (carboxin-thiram). The efficacy of phenazine producing a wild type strain of P. aureofaciens was higher than its non-phenazine producing mutant, indicating that phenazine plays an important role in the antagonistic activity of P. aureofaciens. Effective bacterial antagonists were then studied for their antagonistic mechanisms. The results showed that all four bacteria employed different mechanisms. The bacteria produced siderophore, and volatile metabolites and non-volatile metabolites, in their antagonistic activities. The results of this study suggest that P. auerofaciens may be a new biocontrol agent for controlling cotton seedling mortality disease.
Six experiments were conducted for the enhancement in seed cotton yield by increasing water use efficiency and water savings in case of different planting methods of cotton (Gossypium hirsutum L.) crop during the years 2014 & 2015, at three different locations in cotton zone of Punjab (Pakistan) province. The planting methods were, flat planting and no earthing up, flat planting and earthing up after 1st irrigation, flat planting and alternate row earthing after 1st irrigation, flat planting in 112.5/37.5 cm apart paired rows and earthing up after 1st irrigation, ridge planting and bed planting. Cotton crop obtained a maximum benefit from the available water at all three locations in case of flat planting with alternate row earthing up (P3) by predicting a maximum water use efficiency up to 6.79 kg ha-1 mm-1and maximum seed cotton yield of 3432.50 kg ha-1. Maximum water savings of 25.60% was recorded in the same method (P3) during the year 2014, while minimum (-0.17%) was observed in ridge planting (P5) at location number 1 in the same year.
The population of spider feeding on insect pests of cotton was observed throughout the growth period of the crop, being maximum in the month of July. Chrysoperla was observed from second fortnight of June to harvesting of the crop being maximum in November. The incidence of coccinellids was recorded from the middle of August to middle of September and remained till harvesting of the crop being maximum in the first fortnight of November. A positive significant effect of maximum and minimum temperature on spider and negative significant effect on population of chrysoperla and coccinellids was recorded. Evening RH exerted positive significant effect on spider and negative significant effect on coccinellids.
A study was done on the feeding behaviour, development indices, and biochemical response of 4th instar larvae of Spodoptera littoralis in which seven cotton genotypes were used: Giza86, Giza88, Giza92, 10229 × Giza 86, H10, Suvin, and Karshenseki. Castor bean leaves were used as the control. All the tested cotton genotypes decreased the feeding behavior of S. littoralis larvae in terms of consumption percentages, consumption rate (CR), growth rates (GR), efficiency of conversion of ingested and digested food (ECI and ECD), approximate digestibility (AD), and feeding deterrence (FDI) compared to the control. Additionally, all the tested cotton varieties prolonged the time taken for larval and pupal duration and reduced both the pupation percentages and the weight of the resulting pupae, as compared to the control. Giza86 recorded the lowest values of both larval growth index and fitness (7.31 and 1.05, respectively) while the genotype Suvin recorded the lowest standardised growth index (0.020) more than other genotypes and the control that gave 10.16, 1.53, and 0.032, respectively. There was a significant inhibition in the level of both total soluble protein and total lipids, and activities of amylase and the trehalase enzymes in all the tested varieties, compared to that found in the control.
Retroviral envelope (env)-like sequences in 2 cultivated allotetraploid cottons and their diploid progenitors have been identified and characterized in this study. DNA sequence analysis reveals that these sequences are heterogeneous. The observed sequence diversity, however, seems to preserve coding information. This is evidenced by the detection of the transmembrane domain (TM), which is the most conserved feature of the divergent retroviral env genes. The high ratio of synonymous to nonsynonymous changes suggests that these sequences are evolving under purifying selection. Phylogenetic analysis shows that Gossypium sequences closely cluster with a lineage of plant endogenous retroviruses that have an env-like gene. These results provide evidence for the antiquity and the wide diversity of env-like sequences in the Gossypium genome.
The fauna of Tenebrionidae (Coleoptera) from the cotton fields of some regions of Iran is studied. In total 24 species of 21 genera including Adelostoma DUPONCHEL, Amnodeis MILLER, Haemerophygus BAUDI, Pachyscelis SOLIER, Pimelia FABRICIUS, Stenosis HERBST, Dailognatha ESCHSCHOLTZ, Tentyria LATREILLE, Dendarus DEJEAN, Gonocephalum SOLIER, Opatrum FABRICIUS, Cabirutus STRAND, Pedinus LATREILLE, Alphitobius STEPHENS, Blaps FABRICIUS, Entomogonus SOLIER, Odocnemis ALLARD, Nalassus MULSANT, Pseudoprobaticus NABOZHENKO, Raibosceles ALLARD and Tenebrio LINNAEUS, and 3 subfamilies (Pimeliinae, Opatrinae and Tenebrioninae) were collected. Nine species were recorded for the first time in Iran.
The cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is a polyphagous sap sucking insect with a wide geographical and host range causing severe losses in economically important crops. This study represents the first record of P. solenopsis as a new insect attacking cotton plants (Gossypium barbadense var. Giza 86) in Kafr El-Sheikh governorate, Egypt. The insect was noticed on cotton plants for the first time during its growing season of 2014. The mealybug specimens were collected from infested cotton plants and identified as P. solenopsis. In an attempt to control this pest, eight toxic materials viz., imidacloprid, thiamethoxam, flonicamid, emamectin-benzoate, chlorpyrifos, methomyl, deltamethrin and mineral oil (KZ-oil), belonging to different chemical groups, were tested for their influence against P. solenopsis on cotton under field conditions. Methomyl, imidacloprid, thiamethoxam and chlorpyrifos showed the highest efficacy against P. solenopsis recording 92.3 to 80.4% reduction of the insect population. Flonicamid, emamectin-benzoate and KZ-oil failed to exhibit sufficient P. solenopsis control.
In view of the ecological hazards of chemicals, pot experiments were conducted to determine the efficacy of Trichoderma sp. against Macrophomina phaseolina. Greenhouse evolution of the interaction between M. phaseolina isolates and Trichoderma sp. (isolated revealed a very highly significant (p=0.0000). M. phaseolina isolate x antagonist isolate interaction for all the following parameters: preemergence damping-off, postemergence damping-off, survival, plant height, and dry weight. This interaction implies that a single isolate of antagonist can be highly effective against an isolate of M. phaseolina, but may have only minimal effects on other isolates of M. phaseolina. Therefore, isolates of antagonist should be tested against as many isolates of M. phaseolina as possible, as this will improve the chance of identifying antagonist isolates effective against several isolates of M. phaseolina.
Five plant oils from sesame (Sesamum indicum), oil-palm (Elaeis guineensis), cotton (Gossypium hirsutum), castor (Ricinus communis) and maize (Zea mays) at a dosage of 5 ml/kg of common bean seeds and a control of malathion dust 2% active substance (a. s.) at a dose 0.5 g/kg of seeds were evaluated for their ability to suppress the populations of Z. subfasciatus. Castor and palm oils resulted in effective protection comparable to that of malathion. There were a significant low percentage seed damage and weight loss in seeds treated with malathion, castor and palm oils. Total number of weevils in these treatments were least, compared to other plant oils studied. All treatments did not show adverse effect on germination capability of the seeds. This study showed that it is possible to use castor or palm oils to protect common bean seeds against Z. subfasciatus infestations. These products can be obtained locally at a reasonable cost.
The ability of fluorescent pseudomonads of cotton rhizosphere of induction of systemic resistance (ISR) against bacterial blight of cotton was investigated. Of the 21 isolates tested, 19 were observed to increase the resistance of plants. This was demonstrated by a lower percentage of infected leaf area. On the basis of growth promotion and ISR induction ability, isolates 148, 35Q, 16Q and 113 were selected for further investigations. All isolates increased the resistance of plants, and per cent of infected area on leaves of these treatments were lower than those control of plants. Levels of peroxidase (PO) and phenylalanine ammonia lyase (PAL) activity in the leaves of bacterized plants with selected isolates were similar to that in control plants, but after inoculation of leaves with the pathogen, the amount of these enzymes increased in bacterized plants to high levels. The increase of enzyme activity in control plants was low after inoculation.
Six isolates of Trichoderma spp. (belonging to species: Trichoderma harzianum and T. longibrachiatum) were applied as seed or soil treatments to suppress damping-off of seedlings of ten cotton cultivars under greenhouse conditions. In most cases, cultivar x isolate interaction was a highly significant (p < 0.01) source of variation in the tested seedling growth parameters: incidence of disease, seedling height, and seedling dry weight. This interaction implies that a single isolate of Trichoderma can be highly effective in controlling the disease on a cotton cultivar but may have minimal efficiency in controlling the disease on another cultivar. It was also found that, in most cases, cultivar x isolate x application method was a highly significant source of variation (p < 0.01) in the tested growth parameters. Cotton cultivars showed differences in the disease reaction to the biocontrol agents. In the experiments evaluating the Trichoderma antagonists and their effect on seedling disease, a highly significant (p < 0.01) experimental treatment interaction was found. This interaction suggests that the outcome of cultivar x isolate interaction is markedly affected by the application method. Thus, the application method should be chosen to maximize the outcome of this interaction. The degree of the control of seedling disease in cotton differed according to the isolates of antagonists, the application method and cultivars.
The cotton leafhopper (Amrasca biguttula biguttula Ishida) is considered to be an alarming insect pest causing both quantitative and qualitative loss in cotton. In situ bioassay studies were done and the role of detoxifying enzymes in conferring resistance to neonicotinoid groups of insecticides in low (MUD), medium (DVG), high (HVR) and very high (GLB) pesticide usage areas of Karnataka were determined. Bioassay studies showed that imidacloprid, thiamethoxam, acetamiprid, thiacloprid and clothianidin registered varying levels of resistance for all the locations studied. The resistance ratio was high in imidacloprid (3.35, 8.57, 9.15 and 12.27 fold respectively) and the lowest in dinoferuran (1.86, 5.13, 6.71 and 9.88 fold respectively). Furthermore, the enzyme activity ratio (glutathione-S-transferase) was relatively greater, and corresponded to the higher LC50 values of neonicotinoids for very high, high, medium and low pesticide usage areas. Our study suggested that the higher activity of the detoxifying enzyme in the resistance population of cotton leafhopper apparently has a significant role in endowing resistance to neonicotinoid groups of insecticides. However, this study recommends using neonicotinoids in cotton growing areas with caution.
Insecticide Resistance Management strategies have been formulated with several cost effective, ecofriendly novel approaches and rotation of insecticides based on resistance data for managing the pest complex for stabilizing the cotton ecosystem and improving the social economic status of the cotton growers. Insecticide Resistance Management (IRM) strategies for managing cotton pest complex revealed that sucking and bollworm complex was lower in IRM fields compared to non IRM fields. The strategic positioning of insecticides coupled with ecofriendly technologies led to abundance of natural enemies in cotton ecosystem in IRM fields, while the incidence of these insects was lower in non IRM fields due to insecticidal sprays. Farmers by adopting IRM strategies realized higher net returns by saving in plant protection cost due to less number of insecticidal sprays and increased seed cotton yield.
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