Bacteria of Campylobacter genus remain most commonly reported gastrointestinal bacterial pathogen in humans in the EU since 2005. The main source of human infections caused by Campylobacter bacteria is the consumption of inadequately prepared, infected food products especially poultry meat, milk and dairy products, and contaminated water. High number of campylobacteriosis may us presume that in near future there will be implemented adequate changes in legislation regarding the microbiological criteria for food products, including the detection of Campylobacter spp. This paper contains literature review concerning identification and differentiation methods of Campylobacter genus in food. There are presented classical methods for Campylobacter spp. detection, based on biochemical and physiological properties and as well as molecular methods – PCR, multiplex PCR, PCR-RFLP, qPCR, dot blotting, FISH, which identifies characteristic DNA sequences.