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The authors carried out in the fieldwork with a new variety of amaranth «Lipetsky». In order to identify qualitative and quantitative laws of manifestation of the total antioxidant activity of amaranth the leaves were collected in different phases of plant vegetation, dehydrated in mild conditions of air-shadow drying and subsequently dried under harsh conditions 105º C to a constant mass of samples. Testing of plant samples was carried out using a coulometric method of titration (author's method) of free bromine radicals generated in the aquatic environment by plant titrant (water extract dried in the shade at a temperature of 22º±3º C of leaves). It was examined the combined samples of 10 samples of plant leaves of different tiers and of different ages with field plot of 10 ha. Biometric averages of plants during the growing season: growth, the weight of plants, number of leaves, the weight of leaves, stem and inflorescence of the plant are given. This shows the dynamics of total antioxidant activity of leaves of amaranth during the growing season. The thermal stability of the total antioxidant activity (TAA) for combined extracts of 10 samples of leaves of different ages and from different parts of plants when they are drying at 105º C was tested. The dynamics of change, TAA aqueous extracts of the dried amaranth samples with different stages of the growing season corresponds to the previously identified trends in the availability of high quantitative values in the phase of mass budding beginning of flowering and minima in the initial growth phases (3-8 leaves) and ripening of seed for different varieties of amaranth. There were revealed the increase in antioxidant activity upon drying amaranth samples at 105 °C, revealed a new type of qualitative dynamics of TAA, while vegetation with a characteristic quantitative changes by more than 50% for the beginning of the growth phase (the first phase of 3-8 leaves) and the final phase of seed maturation.
In this study, the total antioxidant status (TAS) was assayed in the blood serum of rats pretreated per os with either N-nitrosodiethylamine (NDEA) (0.1 mg/kg b.w./day) or N-methyl-N-nitrosourea (NMU) (0.1 mg/kg b.w./day) for 30 days. The animals were also dosed per os with spermidine (SPR) (10 mg/kg b.w./day) for a first 21 day period, and Nto-nitro-L-arginine methyl ester (L-NAME) (10 mg/kg b.w./day) given to animals for 3 days (days 22-24), respectively. Nitric oxide synthase (NOS) inhibitor, L-NAME was found to mitigate TAS levels in the blood serum of rats pretreated with NDEA and NMU. No such changes were found in animals dosed with L-NAME only nor even with L-NAME and spermidine, respectively. Since spermidine, also known as an inhibitor of iNOS synthesis, elevated TAS levels in rats dosed with L-NAME and NDEA/NMU, the polyamine was suggested to modify the NOS/NO origin to serve the physiological level of the total anti-oxidant status in rat blood serum.
Eight important red wine grape varieties (Alicante, Kuntra Karasakiz, İrikara, Gabarnet Franch, Cinsaut, Gamay, Merlot and Syrah) which are use for production for red wine by Doluca winery were analyzed. The highest AA (percentage of inhibition on peroxidation in linoleic acid system) was obtained in Gabarnet Franch and Merlot extracts as 90.25% and 90.15% respectively. The lowest AA was determined in İrikara (83.20%) which had the lowest phenol content. Total phenol content (TP) was varied between 1376 (İrikara) and 2329 µg × ml-1 GAE equivalent in methanol extracts (Merlot). Total anthocyanin content (TA) was ranged from 253.5 mg × kg-1 (İrikara) to 2488.4 mg × kg-1 (Alicante). The lowest PPO activity was found in Merlot (0.070 U × ml-1 × min) and the highest activity in Gamay (1.155 U × ml-1 × min). Total sugar content in the analyzed varieties was changed from 15.30% (Syrah) to 22.64 (Merlot). The examinations showed that AA and TP significant correlation (r2 = 0.854**) but less significantly related with TA (r2 = 0.263).
This study was conducted to investigate the effects of pre-harvest Harpin treatments and post-harvest MAP treatments on fruit quality attributes and bioactive compounds of ‘0900 Ziraat’ cherry cultivar throughout cold storage and shelf life of the fruits. Weight loss and decay ratios were significantly reduced with Harpin and MAP treatments. In general, lower L* and chroma values were measured in Harpin-treated fruits. As compared to control fruits, higher firmness, titratable acidity and vitamin C contents were measured in MAP and Harpin + MAP treated fruits throughout the cold storage and shelf life. On the other hand, lower solible solids content (SSC) values were observed in the same treatments. At harvest and shelf life measurements, higher total monomeric anthocyanin (TMA) and total phenolics (TPs) were measured in Harpintreated fruits than in control fruits. Although lower anthocyanin contents were obtained from MAP-treated fruits throughout the cold storage, higher values were observed throughout shelf life. Throughout cold storage and shelf life, MAP-treated fruits had lower total phenolics and total antioxidant activity (according to FRAP and TEAC assay) values than the control and Harpin-treated fruits. It was concluded that Harpin and MAP treatments could be used reduce weight losses and decays throughout the cold storage.
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