Wyniki wyszukiwania

1

Production of bacteriocin by Carnobacterium divergens in batch and continuous culture

100%

Sip A., Grajek W., Boyaval P.

Polish Journal of Food and Nutrition Sciences

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1999

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tom 08

|

nr 3

27-38

Bacteriocin production by Carnobacterium divergens was investigated in batch and continuous fermentation. The best batch bacteriocin productivity, obtained at 25 to 30°C and 2 % inoculum, was 2,580 AU cm-3 h-1 with no pH regulation and 68,270 AU cm-3 h-1 with pH controlled at 6.5. The cell division occurred faster at 37°C. Heat sterilization of broth impaired the biomass production but favoured the bacteriocin production. A large part of the bacteriocin molecules adsorbed on the cells after centrifugation and could be released to the medium by an acidic shock. Filtration of bacteriocin solution caused severe losses of activity. Continuous fermentation allowed to obtain a high bacteriocin activity, reaching 819,200 AU cm-3, at dilution rate 0.12 h-1 and biomass concentration, 3.0 g dm-3

2

Stabilnosc preparatow bakteriocyny bakterii Carnobacterium divergens AS7 podczas przechowywania

94%

Sip A., Grajek W.

Acta Scientiarum Polonorum. Biotechnologia

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2006

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tom 05

|

nr 1-2

75-85

3

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Production of divercin by Carnobacterium divergens in the presence of divercin-sensitive bacteria Carnobacterium piscicola and Listeria innocua

84%

Sip A., Grajek W.

Polish Journal of Food and Nutrition Sciences

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1997

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tom 06

|

nr 3

59-64

The effect of growth of the divercin-producing strain of Carnobacterium diver- gens in the presence of divercin-sensitive bacteria Carnobacterium piscicola and Listeria innocua F on the divercin production was studied. The divercin- -sensitive bacteria were introduced into the growth medium of C. divergens in the form of autolysate or living culture. The biomass yield of C. divergens, its fermentative activity and extracellular divercin production were evaluated. The presence of C. piscicola and L. innocua F was found to inhibit the growth of C. divergens, not affecting the acid production. The divercin-sensitive bacteria enhanced considerably the production of divercin by C. divergens, thus can be considered as divercin-biosynthesis stimulators. The stimulating effect had only autolysates of both divercin-sensitive bacteria. The divercin production in the presence of divercin-sensitive bacteria was dependent on the age of C. divergens culture.

4

Scale-up of divercin production by Carnobacterium divergens AS7 in stirred tank reactors

84%

Sip A., Dembczynski R., Bialas W., Juzwa W., Czaczyk K., Grajek W., Jankowski T.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2008

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tom 11

|

nr 1

5

The effects of Carnobacterium divergens AS7 bacteriocin on gastrointestinal microflora in vitro and on nutrient retention in broiler chickens

84%

Jozefiak D., Sip A., Kaczmarek S., Rutkowski A.

Journal of Animal and Feed Sciences

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2010

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tom 19

|

nr 3

The present study was undertaken to evaluate the effects of divercin, a bacteriocin produced by lactobacilli strain Carnobacterium divergens AS7, on the microflora status under in vitro conditions and on nutrient retention and nitrogen-corrected apparent metabolizable energy (AMEN ) of divercin in an in vivo trial on broiler chickens. Low (DL) 200 AU·ml1 (0.05% of the liquid divercin prepatation), and high (DH) 1600 AU·ml-1(0.4% of the liquid divercin prepatation) doses of divercin were used in both trials. In the in vitro trial divercin at concentration, 1600AU ml-1 of divercin had stronger antibacterial effects as compared with 200 AU·ml-1. In the crop and ileal digesta, the DH treatment was characterized by the lowest lactic acid bacteria (LAB) and coliform bacteria counts (0.4-0.8 log cycle reduction). There were no differences in nutrient retention between treatments. Salinomycin and divercin supplementation tended to increase fat digestibility and N retention. However, the highest AMEN were obtained in the DL treatment. The results of both studies show positive effects of divercin in terms of reduction of microbial populations isolated from the gastrointestinal tract (GIT) of broiler chickens as well as improvement in AMEN. The presented data may suggest that bacteriocin derived from Carnobacterium divergens AS7 could play a role in controlling the microbial ecosystem in the broiler chicken GIT.

6

Production of dry divercin preparations by spray drying and lyophilization

84%

Dembczynski R., Lubbe P., Bialas W., Sip A., Jankowski T., Grajek W., Marecik R., Cyplik P.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2008

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tom 11

|

nr 2

7

Aktywnosc bakteriocyny wytwarzanej przez bakterie Carnobacterium divergens AS7 w mielonym miesie wolowym

84%

Sip A., Grajek W.

Folia Universitatis Agriculturae Stetinensis. Scientia Alimentaria

|

2005

|

nr 04

255-265

8

Production of bacteriocin by Carnobacterium divergens grown in culture media containing hydrolysates of whey, casein and malt roots

84%

Grajek W., Bobowicz-Lassocinska T., Sip A.

Polish Journal of Food and Nutrition Sciences

|

1996

|

tom 05

|

nr 4

A bacteriocin divercin was produced in batch cultures by Carnobacterium divergens. The bacteria were grown in the liquid MRS media containing enzymatic hydrolysates of whey, casein or malt roots as organic nitrogen sources. The divercin production was greatly affected by the medium composition. The highest divercin activity (12,800 AU/mL), measured by the critical dilution assay using C. piscicola as an indicatory microorganism, was obtained in the medium containing whey, casein hydrolysates, and glucose. Omission of glucose in this medium delayed the divercin biosynthesis only for 5 h. The medium developed was more efficient than the MRS medium used commonly for the bacteriocin production.

9

Biosynthesis of divercin by Carnobacterium divergens AS7 in continuous high cell density cultures

67%

Sip A., Grajek W.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2003

|

tom 06

|

nr 1

10

Transfer of divercin through ultrafiltration membranes after treatment with detergents and organic solvents

67%

Sip A., Grajek W.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

|

2001

|

tom 04

|

nr 2

The ultrafiltration of post-culture fluids proved that divercin occurred in the form of large macroaggregates with the molecular weight over 100 kDa. Addition of detergents: Tween 80, Nonidet P-40 and SDS, and alcohols: ethanol and isopropanol into supernatants from post-culture fluids caused partial disaggregation of divercin. The better effect was obtained when Tween 80 was introduced directly into culture medium. However, other detergents and both alcohols showed a toxic effect to the bacteria and reduced the divercin production in C. divergens culture. The ultrafiltration yield of divercin depended on the type of membranes and treatment of divercin-containing extracts with detergents and alcohols. The cellulose acetate membranes were more permeable for divercin that made from polyethersulfone.

Ograniczanie wyników

Production of bacteriocin by Carnobacterium divergens in batch and continuous culture

Stabilnosc preparatow bakteriocyny bakterii Carnobacterium divergens AS7 podczas przechowywania

Production of divercin by Carnobacterium divergens in the presence of divercin-sensitive bacteria Carnobacterium piscicola and Listeria innocua

Scale-up of divercin production by Carnobacterium divergens AS7 in stirred tank reactors

The effects of Carnobacterium divergens AS7 bacteriocin on gastrointestinal microflora in vitro and on nutrient retention in broiler chickens

Production of dry divercin preparations by spray drying and lyophilization

Aktywnosc bakteriocyny wytwarzanej przez bakterie Carnobacterium divergens AS7 w mielonym miesie wolowym

Production of bacteriocin by Carnobacterium divergens grown in culture media containing hydrolysates of whey, casein and malt roots

Biosynthesis of divercin by Carnobacterium divergens AS7 in continuous high cell density cultures

Transfer of divercin through ultrafiltration membranes after treatment with detergents and organic solvents