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The rats fed for two generations F₀/F₁ with 80%wheat or rye diet, were kiled in the age of 3 mouths of F₁ and blood serum was taken. The activity of cholinesterase, ceruloplasmine, basic phosphatase and Kunitz antitrypsic activity were measured. Blood serum protein, protein electrophoretic fractions (albumins to globulina ratio) and cholesterol levels were estimated. No difference of ceruloplasmine, basic phosphatase activities and total cholesterol levels in rats fed rye or wheat was noted, which proves no changes in the internal liver transport. However the singnificant (p=0.001) difference in choliesterase activity and total protein level in blood serum showes inhibition of protein synthesis in the liver.
Specially selected rye lines with alkylresorcinols (AR) content reduced to 300-600 mg/kg making up 90% of the experimental diet did not enhance the growth of experimental animals, and the utilization of nutrients was not better than in the control experiments with rye containing 1300 mg alkylresorcinols per kg. Alkylresorcinols isolated from rye, wheat and triticale grain, differing as to short-chain and unsaturated homologues composition, were applied in doses of 1 and 2 g/kg casein diet, leading to a significant decrease of growth indices in the case of the higher dose (2 g), irrespective of the contents of homologues.
The excretion of alkylresorcinols ARs in faeces and urine of rats fed with diets containing wheat or rye bran on 3 different levels was studied. Additionaly the effect of wheat and rye bran extracted with acetone was examinated. It was shown that about 36% alkylresorcinols present in wheat diet and about 48% of these compounds present in rye diet were excreted in faeces in intact form. The linear regretion of intake against excreted ARs both of wheat or rye was observed. Chromatographic analysis showed the presence in urine of three distinct fractions of alk/en/ylresorcinol meta bolites.
Trypsin inhibitor (TI) inactivation and alkylresorcinols extraction by washing with acetone did not improve digestibility of protein from wheat grain and bran. Addition of TI isolated from triticale and rye grain to the diet of experimental rats caused statistically significant reduction of digestibility of protein (TD) from casein at doses over 2 TI units per g of grain. TI isolated from rye added to chicken diet in doses of 0.75. 1.5 and 3.0 TI units/g feed did not lead to changes in weight increment and feed consumption during a 10-day experiment.
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