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Acid and alkaline phosphatase and phytase activities were determined in the bacteroid free fractions of chickpea (Cicer arietinum L.) nodules at 15 days intervals, from 40 days after sowing (DAS) to 85 DAS. In general, the activities and specific activity of both the acid and alkaline phosphatases declined at 55 DAS. Out of the various substrates studied, ATP was the best substrate for both phosphatases. Activities of phosphatases with glucose-6-phosphate and fructose-6-phosphate were low in comparison to these with fructose 1,6 bisphosphate. The efficiency of acid phosphatase for utilizing fructose 1,6 bis phosphate as a substrate increased with nodule development. A fructose 1,6 bis phosphate specific acid phosphatase with elution volume to void volume (Ve/Vo) ratio of around 2.0 was observed in mature nodules (80 DAS). Acid phosphatase at 40 DAS was resolved into two peaks which were eluted at Ve/Vo of about 1.5 and 1.8. However, at 60 DAS the peak with Ve/Vo of 1.5 could not be detected. With ATP as substrate, a high (Ve/Vo of 1.2) and low MM form (Ve/Vo of 2.1) alkaline phosphatases were observed at 40 DAS however at 60 DAS stage only one peak with Ve/Vo of 1.7 was detected. Although, a low activity of acid phytase was observed in nodules at all stages of development but neither alkaline phytase nor phytic acid could be detected. It appears that the nodules acquire inorganic phosphate from the roots. The higher content of water soluble organic phosphorus in mature nodules could be due to the low activities of phosphatases at maturity.
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Phytate-degrading activity in lactic acid bacteria

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The phytate-degrading and phosphatase activities of several Lactobacillus strains belonging to different species and isolated from different ecosystems were tested. The specific activities against phytate (InsP6) varied from 0.024 to 0.531 U/mg protein, being the highest for L. casei DSM 20011. The studied strains hydrolysed p-nitrophenylphosphate at higher rates than phytate, except for L. casei DSM 20011 and L. plantarum W42. The ability of the different strains to hydrolyse InsP6 and generate lower myo-inositol phosphates during growth was tested by HPLC. The InsP6 hydrolysis was in the range from 0.0 to 8.83%, being the highest for L. plantarum W42 and L. plantarum 110, followed by L. casei 40W. The optimal pH and temperatures of phytate-degrading activity varied in the range from 5.0 to 7.5 and from 50 to 60°C, respectively. The incorporation of different types of carbon sources or inorganic phosphate to the growth medium modulated the synthesis of phytate-degrading enzymes in the studied strains. Further studies should be carried out to provide progress in the understanding of the potential nutritional and technological roles of the most active strains in the elaboration of whole sour breads.
The objective of the study was to evaluate the effects of dietary phytic acid (IP6) on the apparent total tract digestibility (ATTD) of main nutrients and apparent absorption of minerals in mink (Mustela vison). A basal moist diet consisting of fish meal, fish oil and wheat starch was added at different concentrations of soluble sodium phytate, thus obtaining six diets with graded levels of IP6 (0-19.9 g IP6 kg dry matter-1). Each diet was fed to four adult male mink kept in individual cages. There were no significant effects of IP6 on the ATTD of crude protein, fat, starch or energy, whereas the apparent absorption of copper and magnesium was significantly reduced with increasing concentration of IP6. Thus, as observed in other monogastric animals, we conclude that dietary IP6 could impair mineral status in mink, but may not affect digestibility of energy-yielding nutrients.
Lactic acid has been known as one of compounds to cause cellular harm in waterlogged tissues through the process of cytoplasmic acidosis. The effects of lactic acid on α-amylase activity and phytic acid (myo-inositol 1,2,3,4,5,6-hexaphosphate) content using an assay for high phosphate in the germination stage of rice were evaluated. It is showed that lactic acid inhibited rice germination at every treated dose. The reduction of α-amylase content attributed to lactic acid at 24 h after germination of rice seeds was observed. The findings highlighted the effects of lactic acid on α-amylase activity and phytic acid content and suggested that this compound may play a potent role as a germination regulator in rice.
The intestinal and total tract digestibilities of phosphorus (P) and phytate P were determined in digestibility trials with laying hens and broiler breeders fed a diet containing P, phytate P and Ca at 6.37, 2.00 and 34.1 g·kg-1, respectively. In both digestibility trials, the total tract digestibility of phytate P was higher than the intestinal digestibility (33 and 35% vs 20 and 18%). In contrast, in both trials the total tract retention of P was lower than the intestinal retention (22 and 19% vs 52 and 42%). Phytate P represented 29.2% of the excreta P of laying hens and 23.6% of the excreta P of broiler breeders. The corresponding proportions of phosphate P were 48.7 and 46.6%, respectively. Samples of digesta and small intestinal mucosa of laying hens were diluted with physiological saline containing sodium phytate and incubated in vitro to determine the phytase activity. The average specific phytase activities in the crop, stomach, small intestinal contents, mucosa and caecal contents were 10.2, 9.2, 14.6, 11.5 and 135 μmol·h-1·g-1, respectively. The total phytase activities (per segment) in the small intestine (including the mucosa) and the caeca were 586 and 663 μmol·h-1, respectively. It can be concluded that phytase activity was present in all sections of the digestive tract. Consequently, in hens fed a wheat-maize-soyabean diet without a phytase supplement, phytate P was partially digestible. A part of the phytate degradation occurred in the hindgut.
The biological bases for the relation of the cereal grains low molecular weight antioxidants intake to the reduction of risk for chronic diseases caused by oxidative stress have been discussed in this article.
Dwie odmiany pszenicy (Triticum aestivum L. odm. Opatka i Korynta) rosły przez 4 tygodnie na podłożach: z łatwo dostępnym, nieorganicznym źródłem fosforu - KН2РO4 (+P, kontrola), trudno rozpuszczalnym nieorganicznym fosforanem - FePO4 (FeP), organicznym źródłem fosforu w postaci kwasu fitynowego (F) oraz bez fosforu (-P). Badano wpływ zróżnicowanego żywienia fosforowego roślin rosnących w obecności partnera grzybowego (Glomus intraradices) jak i roślin nie zainfekowanych grzybem, na parametry wzrostowe i zawartość fosforu całkowitego w tkankach. U obu badanych odmian stwierdzono raczej negatywny wpływ mikoryzacji na wzrost roślin w warunkach zróżnicowanego żywienia fosforowego. Wyższą suchą masę pędów (w porównaniu do roślin niezainfekowanych) obserwowano jedynie u roślin odmiany Korynta rosnących na podłożu zawierającym fitynę. Zaobserwowano niskie stężenie fosforu całkowitego w pędach i korzeniach obu odmian rosnących na podłożach bez fosforu oraz z fosforanem żelaza. Deficyt fosforu w tkankach spowodował zmiany relacji wzrostowych zarówno roślin rosnących na podłożach bez fosforu z fosforanem żelaza jak i kwasem fitynowym. Nie zaobserwowano istotnych różnic pomiędzy badanymi odmianami pszenicy.
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