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The aim of the present work was to recognize the reasons for differences in the photodynamic action of dyes against various bacterial strains. It is expected that a better understanding of this problem may help in design of new photosen-sitizers. The sensitivity of 6 various bacterial strains to the photodynamic action of 5 photosensitizers was determined. The hydrophobicity of cell surface and susceptibility of bacteria to the natural defense mechanism of human serum, were estimated. The differences in the photodynamic efficiency of dyes could be contributed to various affinities of cell membrane to dyes, to known details of membrane architecture as well as to different mechanisms of photosensitization.
Starch hydrolysates were prepared by hydrolysis of potato and corn starch mixture with a heat-stable bacterial alpha amylase (Termamyl 120 LS) or with a heat-stable bacterial alpha amylase (Termamyl 120LS) and next with a fungal alpha amylase (Finizym 1600L). Additionally so-called filtration enzyme action was applied (Finizym, Neutrase, Lecitase, SP 348, Gammazym LPL). Introduction of these enzymatic preparations influenced on the increase of filtration rate of hydrolysates and decrease of their viscosity and transmittance value. “Filtration enzyme” did not influence on the carbohydrate composition.
Population dynamics in batch and continuous culture of Shigella flexneri 3b lysogenic for plaque-less phage X and sensitive to this phag lb serovars have been determined. The abrupt change in the fraction of lysogens resulting from the death, especially of sensitive cells, was observed. The death should result of spontaneous liberation of phage X as concluded from the growth dynamics of the sensitive strain infected with the phage containing bacteria-free filtrate of 3b lysogen. Basing on experimental data mathematical modelling of the dynamics with the use of the differential equation set has been proposed. In the models a qualitative relation between the strains and the phage, estimation of growth parameters and estimated growth curves are given.
The rep-PCR fingerprinting method, with the support of ERIC and REP primers, was used to analyse the genomic diversity of 93 E. coli strains isolated from lake water samples drawn at two different depths. The applied UPGMA for DNA analysis did not reveale any genomic similarities between the 48 E. coli strains derived from the subsurface-zone water and the 43 of the bottom-zone water. The considerable genomic diversity of the E. coli of the surface zone was expressed as a dendrogram in the form of 8 similarity groups comprising strains isolated from samples drawn over one month. The bottom-zone strains, which display a lesser degree of genomic diversity (5 similarity groups), showed distinct common features in their DNA fingerprints. In the similarity dendrogram for the bottom-zone, strains derived in different months of sampling were segregated into the same similarity groups. Applying REP primers in rep-PCR generates more complex fingerprints increasing the discriminatory power of the analysis, whereas the ERIC primer generates less complex fingerprint patterns, and is thus clearer to interpret.
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