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Circadian and seasonal changes in the susceptibility of honeybee (Apis mellifera) workers to intoxication by organophosphate, benzoylphenyl urea, carbamate, and oxadiazine insecticides have been studied from 2008 to 2009. Animals were collected in two apiaries located in the surroundings of Mielec and Tarnobrzeg in the Sub-Carpathian Province. The experiments consisted of intoxication by select xenobiotics of the subsequent groups of animals in two-hour intervals for a period of 24 hours and the analysis of their survivability. Experiments were carried out in spring and summer. The results suggest that the susceptibility of bees to different groups of insecticides varies significantly, both in the circadian and seasonal rhythms.
In the years 2009 and 2010, in the apiaries surrounding Tarnobrzeg and Leżajsk, Poland (close to the Carpathian Mountains) research was carried out on diurnal changes in the sensitivity of young honey bee (Apis mellifera) workers to insecticides from various chemical groups: pyrethroids (esfenvalerate, cyhalothrin, alpha-cypermethrin, beta-cyfluthrin, deltamethrin), derivatives of pyridine (pyriproxyfen), carbamate (pirimicarb), organophosphate (diazinon), and benzoyl urea derivative (teflubenzuron). The analyses consisted of intoxicating subsequent groups of honey bees in 2-hour intervals, for a period of 24 hours with selected xenobiotics. The results received indicate that the honey bee shows a statistically significant susceptibility to insecticides, changing in the diurnal rhythm.
We examined the susceptibility of the worker honeybee Apis mellifera L. to pyrethroids. Bees were intoxicated by selected synthetic pyrethroids (active substances: beta-cyfl uthrin, deltamethrin, alphacypermethrin, lambda-cyhalothrin, esfenvalerate, and bifenthrin) and 72 hours after intoxication the number of surviving insects was determined. It was observed that the susceptibility of the honeybee to pyrethroids (expressed herein as the survival rate) was changing in diurnal rhythm, ranging from 100% (for a majority of substances in both seasons) to 11.1±0.37% (deltamethrin) and 11.1±0.73% (esfenvalerate) in spring, and 11.1±0.73% (esfenvalerate) and 4.6±0.17% (bifenthrin) in summer. The lowest susceptibility of bees to pyrethroids occurred during the night, when the honeybees were characterized by reduced motility and did not fl y out for foraging, whereas the highest susceptibility was observed during the day, a natural time for seeking food. Along with diurnal changes in the susceptibility described above, differences dependent on the seasons in which the intoxication took place were also observed. Insects intoxicated in summer were less susceptible to pyrethroids than those intoxicated in spring.
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