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In vitro micropropagation of 'Khalas' date palm [Phoenix dactylifera L.], an important fruit plant

100%

Aslam J., Khan S. A.

Journal of Fruit and Ornamental Plant Research

2009

tom 17

nr 1

15-27

A protocol has been developed for an efficient tissue culture cycle (callus induction, callus growth, plant regeneration and root induction) for date palm 'Khalas' using various plant growth regulators (PGR) in both liquid and solidified MS medium. The shoot explants showed high callus induction potential on MS medium supplemented with 45.24 ^M 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 54.21 ^M 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). The highest frequency of shoot regeneration (85%) and number of shoots per an explant (5.6) were obtained on solid MS medium supplemented with 7.84 ^M N6-benzylaminopurine (BAP). Rooting (87.34%) was high on solid MS medium supplemented with 24.6 ^M indole-3-butyric acid (IBA). However, the root length was higher in MS liquid medium. This protocol will be useful for rapid, large-scale propagation of 'Khalas' date palm.

Influence of freezing and non-freezing temperature on somatic embryogenesis and vinblastine production in Catharanthus roseus (L.) G.Don.

80%

Aslam J., Mujib A., Sharma M. P.

Acta Physiologiae Plantarum

2011

tom 33

nr 2

Catharanthus roseus (L.) G. Don. (Apocynaceae) is an important dicotyledonous medicinal plant. It produces vinblastine and vincristine, two alkaloids that are being used against a variety of cancers. In the present study, the freezing (-196, 4, 15°C) and non-freezing (25°C) temperature was imposed on embryogenic cultures, and later in vitro embryogeny and vinblastine production in C. roseus was studied. Somatic embryo (SE) production was maximum at 15°C, but the SE maturation was high at 4°C. The SEs, grown at 25°C, showed highest germination and plantlet conversion. Quantitative estimation of vinblastine was carried out using high-performance liquid chromatography in various in vitro raised tissues (embryogenic callus), embryo stages (proliferated, matured and germinated embryos)], and SE-derived plantlets (leaf, shoot, root and whole plant) after various freezing- and non-freezing temperature treatments. Vinblastine synthesis was temperature dependent in C. roseus that has been discussed in this present article.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Journal of Fruit and Ornamental Plant Research

2 Aslam J.

1 Khan S. A.

1 Mujib A.

1 Sharma M. P.

1 2011

1 2009

Wyniki wyszukiwania

In vitro micropropagation of 'Khalas' date palm [Phoenix dactylifera L.], an important fruit plant

Influence of freezing and non-freezing temperature on somatic embryogenesis and vinblastine production in Catharanthus roseus (L.) G.Don.