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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effect of the iron chelate FeEDDHA on Staphylea pinnata shoot multiplication and photosynthetic pigment concentration

100%
Szewczyk-Taranek B., Pawlowska B., Prokopiuk B., Moczulska K.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
2

Micropropagation of Harpagophytum procumbens [Burch.] DC. ex Meisn.: the effect of cytokinins on shoot multiplication

100%
Grabkowska R., Wysokinska H.
Herba Polonica
|
2009
|
tom 55
|
nr 3
244-250
The procedure of in vitro propagation of Harpagophytum procumbens using shoot tips was developed. Shoot tips were cultured on Schenk and Hildebrandt (SH) agar medium supplemented with 0.57 µM indole-3-acetic acid (IAA) in combination with various cytokinins: 6-benzylaminopurine (BAP), thidiazurone (TDZ), kinetin or zeatin at four concentrations (2, 4, 6 or 8 µM). The best shoot multiplication rate (11.2 shoots/explant for 5 weeks) was achieved in the presence of 6 µM TDZ. The shoots were small and their elongation on SH medium supplemented with gibberellic acid (GA3 ) was necessary. Shoots of H. procumbens were rooted on full-strength or half-strength Murashige and Skoog (MS) agar medium either with or without auxin. Plantlets were transferred into pots and maintained in the greenhouse. After 1 month, the overall survival of plants was 83% but it decreased to 27% after 6 months.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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In vitro culture of Pelargonium

100%
Hanus-Fajerska E., Mrzyglod A., Wiszniewska A., Stolarczyk P.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.

84%
Grzegorczyk I., Bilichowski I., Mikiciuk-Olasik E., Wysokinska H.
Acta Societatis Botanicorum Poloniae
|
2006
|
tom 75
|
nr 1
11-15
This report describes the effect of triacontanol on shoot multiplication and production of antioxidant compounds (carnosic acid, carnosol and rosmarinic acid) in S. officinalis cultures grown on MS basal medium (agar solidified medium supplemented with 0.1 mg l-1 IAA, 0.45 mg l-1 BAP). It was found that shoot proliferation significantly increased when triacontanol at concentrations of 5, 10 or 20 µg l-1 was added to the medium. HPLC analysis of acetone and methanolic extracts of sage shoots showed that the production of diterpenoids, carnosic acid/carnosol ratio, as well as, contents of rosmarinic acid were also affected by the treatment with triacontanol. The highest stimulation effect of triacontanol was observed on the production of carnosol, where the treatment with 20 µg l l-1 increased the content of this diterpenoid 4.5-fold compared to that in the control (sage shoots growing on MS basal medium, only).
5

The post-effect of in vitro storage on shoot multiplication and rooting 'Senga Sengana' strawberry shoots

84%
Lisek A., Orlikowska T.
Journal of Fruit and Ornamental Plant Research
|
2008
|
tom 16
123-132
6

In vitro regeneration and somaclonal variation of Petunia hybrida

84%
Abu-Qaoud H., Abu-Rayya A., Yaish S.
Journal of Fruit and Ornamental Plant Research
|
2010
|
tom 18
|
nr 1
71-81
The effect of different levels of naphthaleneacetic acid (NAA) and benzyladenine (BA) on shoot multiplication and regeneration of Petunia hybrida was studied. Regenerated shoots from leaf explants were grown ex vitro for analysis of somaclonal variation. Seeds of Petunia hybrida were germinated in vitro on MS basal medium. The seedlings were used as a source of explants for the multiplication and regeneration experiments. For the shoot multiplication experiment, shoot explants taken from germinated seedlings were cultured on MS basal media supplemented with different concentrations of BA (0.1, 0.4, 0.8 mgl-1) and NAA at concentration of 0.1 mg l-1. Highest number of axillary shoot was obtained on medium supplemented with 0.8 mg l-1 BA and 0.1 mgl-1NAA. For regeneration experiments, leaf sections taken from germinated seedlings were cultured onto MS media supplemented with three levels of BA (0.5, 1.0 and 2.0 mgl-1) and 0.5 mgl-1NAA. The highest shoot regeneration rate (45%) was observed in MS medium supplemented with 2 mg l-1 BA. For the evaluation of somaclonal variation, lateral bud explants were taken from pink colored petunia plants that were grown in the greenhouse. The buds were disinfected and cultured on MS basal media supplied with 30 mgl-1 gentamycin sulfate and 30 mg l-1 Benlate. After shoots grew, leaf sections were then taken from them shoots and cultured onto shoot regeneration medium (MS medium supplemented with 2 mg l-1 BA). The regenerated adventitious shoots were cultured on MS medium without growth regulator. These shoots were then rooted, acclimatized and transferred to the greenhouse for evaluation. Two forms of leaf shape (orbicular and elliptic) and three flower colors (violet, purple and light pink) appeared on the plantlets.
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Comparison of Allium aflatunense B. Fedtsch. ‘Purple Sensation’ and Allium karataviense Regel. ‘Ivory Queen’ regenerative capabilities in tissue culture

84%
Kozak D., Stelmaszczuk M.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2013
|
tom 12
|
nr 6
Allium aflatunense and Allium karataviense are valuable bulbous plants grown in a garden. Moreover, Allium aflatunense is one of the most decorative species for cut flowers industry. These plants characterize with a low multiplication rate. Immature inflorescence stem sections and leaf bases isolated from ‘Allium aflatunense ‘Purple Sensation’ and Allium karataviense ‘Ivory Queen’ bulbs in October were used as source of explants for shoot regeneration. The use of a two – step sterilization procedure (2% Cl- – 30 min and 1% Cl- –15 min) gave much better results than a one – step (2% Cl- – 30 min). Inflorescence explants in inverted orientation were cultured on a Murashige and Skoog [1962] medium containing BA in concentration of 2 mg·dm⁻³ and NAA in concentration of 1 mg·dm⁻³. Leaf bases placed in normal and inverted orientation were incubated on MS medium supplemented with: BA 2 mg·dm⁻³ plus NAA 1 mg·dm⁻³, BA 5 mg·dm⁻³ plus NAA 1 mg·dm⁻³ and TDZ 1 mg·dm⁻³ plus NAA 1 mg·dm⁻³. In subsequent experiments clumps consisting of 2 shoots were cultured on MS medium containing BA 0.5, 1, 2 mg·dm⁻³ with NAA 0.1 mg·dm⁻³ and BA 2 mg·dm⁻³ with IAA 0.1 mg·dm⁻³ for 6 weeks. A multiplication rate during four subcultures on medium with addition of BA 0.5 mg·dm⁻³ and NAA 0.1 mg·dm⁻³ was also examined. Explants from young inflorescences and from immature leaf bases were found effective to form shoots. The orientation of leaf explants did not have a significant effect on the number of regenerating shoots. 5.7 to 7.5 shoots were achieved for Allium aflatunense ‘Purple Sensation’ on studied media during 6 weeks of multiplication and 3.5 to 5.1 shoots for Allium karataviense ‘Ivory Queen’. Multiplication rate of 31.3 shoots for Allium aflatunense ‘Purple Sensation’ and 26.6 shoots for Allium karataviense ‘Ivory Queen’ was possible to obtain on medium supplemented with BA 0.5 mg·dm⁻³ and NAA 0.1 mg·dm⁻³ after 32 weeks of culture.
8

Micropropagation of Physalis ixocarpa L.

84%
Bergier K., Wielanek M., Gajewska E., Sklodowska M.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2007
|
tom 523
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Influence of iron sources in the nutrient medium on in vitro shoot multiplication and rooting of magnolia and cherry plum

84%
Sokolov R. S., Atanassova B. Y., Iakimova E. T.
Journal of Horticultural Research
|
2015
|
tom 23
|
nr 2
10

Effect of controlled carbon dioxide on in vitro shoot multiplication in Feronia limonia [L.] Swingle

84%
Vyas S., Purohit S. D.
Acta Physiologiae Plantarum
|
2006
|
tom 28
|
nr 6
The effect of controlled carbon dioxide environment on in vitro shoot growth and multiplication in Feronia limonia (a tropical fruit plant, Family- Rutaceae) was studied. Carbon dioxide available in the ambient air of the growth room was insufficient for in vitro growth of the shoots alone. Also, the presence of sucrose only as the C-source in the medium (without CO2), was found to be inadequate for sustainable growth and multiplication of shoots. The carbon dioxide enrichment promoted shoot multiplication and overall growth. The promotory effect of CO2 was independent of the presence of sucrose in the medium. In the presence of both CO2 and sucrose, an additive effect was observed producing maximum shoot growth. In the absence of sucrose a higher concentration of CO2 (10.0) gm⁻³ was required to achieve photoautotrophic shoot multiplication comparable to ambient air controls. Highest leaf area per shoot cluster promoting shoot growth and multiplication was recorded under this treatment. Shoots growing on sucrose containing medium under controlled CO2 environment of 0.6 gm⁻³ concentration evoked better response than ambient air controls (shoots growing on sucrose containing medium) in growth room. This treatment produced the overall best response. The present study highlighted the possibility of photoautotrophic multiplication which might prove useful for successful hardening and acclimatization in tissue culture plants.
11

Effect of cytokinins and auxins on micropropagation of Clitoria ternatea L.

84%
Rout G. R.
Biological Letters
|
2004
|
tom 41
|
nr 1
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Micropropagation of Stryphnolobium japonicum

67%
Kobus M., Kulpa D.
Dendrobiology
|
2009
|
tom 61
23-26
Observations of Japanese pagoda trees indicate that they undergo a full cycle of vegetative and generative development without self-renovation. The aim of this study was to obtain a successive media protocol propagation of Japanese pagoda tree by in vitro cultures. The effects of growth regulators were studied with reference to primary regeneration, shoot multiplication and rooting. As explants source were used the part of shoot of 90-year-oldtree. Explants were placed on MS (Murashige and Skoog 1962) basal medium with the addition of 6-benzylaminopurine – BAP (1.0–2.0 mg dm-3), thidiazuron – TDZ (0.1–0.3 mg dm-3) and indole-3-acetic acid – IAA (0.5–1.0 mg dm-3). BAP was the growth regulator which significantly increased shoot regeneration on initial explants. TDZ in turn, inhibited the formation of adventitious shoots and caused the explants which had been placed on the medium to die. The multiplication of the Japanese pagoda tree by in vitro cultures should be conducted on MS media with the addition of 0.5 mg dm-3 BAP, and they should be rooted on media with the addition of 0.3 mg dm-3 indole-3-butyric acid – IBA. It seems, that devising an efficient method of Japanese pagoda tree micropropagation is realistic.
13
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

In vitro shoot proliferation of Passiflora caerulea L. via cotyledonary node and shoot tip explants

67%
Jafari M., Daneshvar M. H., Lotfi A.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2017
|
tom 98
|
nr 2
14

In vitro micropropagation of 'Khalas' date palm [Phoenix dactylifera L.], an important fruit plant

67%
Aslam J., Khan S. A.
Journal of Fruit and Ornamental Plant Research
|
2009
|
tom 17
|
nr 1
15-27
A protocol has been developed for an efficient tissue culture cycle (callus induction, callus growth, plant regeneration and root induction) for date palm 'Khalas' using various plant growth regulators (PGR) in both liquid and solidified MS medium. The shoot explants showed high callus induction potential on MS medium supplemented with 45.24 ^M 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 54.21 ^M 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). The highest frequency of shoot regeneration (85%) and number of shoots per an explant (5.6) were obtained on solid MS medium supplemented with 7.84 ^M N6-benzylaminopurine (BAP). Rooting (87.34%) was high on solid MS medium supplemented with 24.6 ^M indole-3-butyric acid (IBA). However, the root length was higher in MS liquid medium. This protocol will be useful for rapid, large-scale propagation of 'Khalas' date palm.
15

Shoot multiplication and plant regeneration of guava [Psidium guajava L.] from nodal explants on in vitro raised plantlets

67%
Rai M. K., Jaiswal V. S., Jaiswal U.
Journal of Fruit and Ornamental Plant Research
|
2009
|
tom 17
|
nr 1
29-38
A micropropagation method is described for guava (Psidium guajava L.) using nodal expiants from somatic embryo-derived young and aseptic plantlets. Multiple shoots were induced from axillary buds on MS medium containing different concentrations of N6-benzylaminopurine (BAP), either alone or in combination with kinetin (Kn), indole-3-acetic acid (1AA) or a-naphthalene acetic acid (NAA). Medium containing 1 mg l-1 BAP was the most effective for shoot multiplication. In vitro regenerated shoots developed roots either on MS medium alone or on MS medium supplemented with indole-3-butyric acid (1BA). The rooted plantlets were successfully acclimatized.
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Micropropagation of Merremia quinquefolia (l.) Hallier F. from nodal explants

67%
Kher M. M., Nataraj M., Parmar H. D., Buchad H.
Journal of Horticultural Research
|
2015
|
tom 23
|
nr 1
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The impact of three bacteria isolated from contaminated plant cultures on in vitro multiplication and rooting of microshoots of four ornamental plants

67%
Zawadzka M., Trzcinski P., Nowak K., Orlikowska T.
Journal of Horticultural Research
|
2013
|
tom 21
|
nr 2
18
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Micropropagation of Grewia tenax (Forssk.) Fiori – an important ethnomedicinal plant

67%
Daffalla H. M., Elsheikh A. M., Ali H. A., Khalafalla M. M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2019
|
tom 100
|
nr 3
19

Effects of carbohydrates on shoot multiplication and bulb formation of tulip in vitro

67%
Podwyszynska M.
Roczniki Akademii Rolniczej w Poznaniu. Ogrodnictwo
|
2001
|
tom 33
20
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effects thidiazuron and paclobutrazol on regeneration potential of tulip flower stalk explants in vitro and subsequent shoot multiplication

67%
Podwyszynska M., Marasek A.
Acta Societatis Botanicorum Poloniae
|
2003
|
tom 72
|
nr 3
The effects of TDZ and paclobutrazol on the primary regeneration on tulip flower stalk explants of six cultivars and subsequent shoot multiplication were examined. Explants, flower stalk slices, were excised from cooled and subsequently forced bulbs. The explants were incubated for two months in darkness on medium containing NAA and cytokinins, 2iP and BAP, as control, or TDZ (0.5-4 mg l-1) and paclobutrazol (0.05-0.4 mg l-1). Then, the regenerating explants were subcultured on medium with TDZ and NAA applied at low concentrations. Different regeneration capabilities were found depending on cultivar and growth regulators. The percentage of explants forming leaf-like structures ranged, on the control medium, from 80% in 'Blue Parrot' and 'Prominence' to below 30% in 'Apeldoorn' and 'Mirjoran'. TDZ, applied at optimum for each cultivar concentration, greatly increased the regeneration potential up to 70-100%. Paclobutrazol, added to the TDZ-containing medium, significantly enhanced the response of explants, resulting in high numbers of leaf-like structures formed per explant (13.7-22.8). The structures developed gradually into characteristic forms: the growing up cotyledonary leaf, the probable root primordium formed at its base, the growing downwards stolon and the shoot meristem developed finely on its tip. It is suggested that such primary regeneration may have a nature of somatic embryogenesis. Then, the adventitious shoots developed and formed clusters, which were divided into 2-3 smaller ones every two months. The growth regulators, used at initial stage, markedly influenced subsequent shoot multiplication. Thus, the most intensive shoot formation was noted with TDZ at concentrations of 0.5-2 mg l-1 and paclobutrazol of 0.05-0.1 mg l-1.
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