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Micropropagation of Plantago camtschatica Link

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The Far East medicinal plant - Plantago camtschatica was propagated in vitro from tips of shoots (obtained in vitro) and from different explants of 4-week-old seedlings: seedling tips, hypocotyls, cotyledons, roots, first leaves. To our knowledge there is no information in literature about in vitro culture of this plantain. MS basal medium, supplemented with 0.6 pM IAA in combination with various cytokinins (BA, KIN, ZEA), was used. After 6 weeks of culture, micropropagation rate (MR) - mean number of buds and shoots per explant - was calculated. Our study proved that P. camtschatica species was amenable to propagation in vitro from different kinds of explants. However, multiplication by adventitious shoot regeneration from hypocotyl explants was found to be the most suitable method for the propagation of this plant. Adventitious shoots could root without stimulation what allows to omit the stage of rooting. The plants obtained as a result of micropropagation were not phenotypically changed.
The species of Pistacia vera, P. mutica and P. khinjuk can be used as rootstocks for pistachio cultivars due to their adaptability to severe environmental conditions and resistance to some pests and diseases. However, low percentage of seedling survival following transplanting has been a major problem. This study was conducted to test the effects of different concentrations of indolebutyric acid (IBA) on root regeneration of transplanted bare-rooted seedlings of these three species. The seedlings treated with IBA at all concentrations tested (1000, 1500, 2000, 2500 and 3000 mg l-1) developed more roots, which were longer and had higher fresh and dry weight, and increased the survival rate of treated seedlings compared to their respective controls. The most effective concentrations in this respect were 2000 and 2500 mg l-1. There was a significant interaction between the species and IBA concentration. Shoot height, root and shoot diameter, leaf number, shoot fresh and dry weight were not affected by IBA. Results also showed that survival rate of the seedlings was significantly correlated with root number, length and fresh and dry weight.
A vanishing species in Poland - Plantago maritima L. was regenerated in vitro from tips of shoots (obtained in vitro) and from different explants of 4-week-old seedlings: seedling tips, hypocotyls, cotyledons, roots. Murashige and Skoog basal medium, supplemented with 0.6 pM indole-3-acetic acid in combination with cytokinins 6-benzyladenine, zeatin or kinetin, was used. The plants obtained in the result of micropropagation were normal in appearence. It was proved that Plantago maritima species was amenable to propagation from different kinds of explants. The method may be of significance for protection of sea plantain.
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