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Thrombolytic and antiplatelet action of Xanthinol nicotinate [Sadamin]: possible mechanisms

100%

Bieron K., Swies J., Kostka-Trabka E., Gryglewski R. J.

Journal of Physiology and Pharmacology

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1998

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tom 49

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nr 2

2

Chemiczna metoda oznaczania kwasu nikotynowego w produktach spożywczych

80%

Markuze Z.

Roczniki Państwowego Zakładu Higieny

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1956

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tom 07

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nr 1

3

Gene expression and oxalate oxidase activity of two germin isoforms induced by stress

80%

Nowakowska J.

Acta Physiologiae Plantarum

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1998

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tom 20

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nr 1

Wheat germin is a homopentameric 125 kD glycoprotein mainly localized in the cell wall of monocots, and is a specific marker of the onset of growth in germinating seeds. The major objective of this study was to examine the expression and oxalate oxidase activity of two wheat germin isoforms: gf-2.8 and gf-3.8 in transgenic tobacco plants. The transgenic tobacco plants were created with different constructs: 1) one entire excision of gf-2.8 germin promoter and two partially deleted promoter sequences were used to generate 3 independent GUS constructs; 2) the whole gf-2.8 gene construct and the fusion with CaMV 35S promoter; 3) one entire excision of gf-3.8 germin gene and one partially deleted gf-3.8 promoter sequences were used to generate 2 independent GUS constructs; 4) the whole gf-3.8 gene and the fusion with CaMV 35S promoter. Hormonal treatment (auxin and gibberellin), salt treatment, heavy metals (Mn, Fe, Co, Ni, Cu, Zn, Cd, Hg, As) and Al induced high GUS activity in tobacco transformed with entire and one partially deleted of the gf-2.8 gene. The immunoblotting confirmed induction of gf-2.8 gene and its product expressed oxalate oxidase activity in tobacco transformed with the entire gf-2.8 construct. Neither nicotinic acid, salicylic acid, heat shock, cold nor UV-C have enhanced significant GUS activity and germin gf-2.8 synhesis and activity. The germin gf-3.8 constructs with GUS gene and with the entire gf-3.8 sequences gave non-positive response with factors mentioned above. It has been demonstrated that gf-3.8 germin isoform is present as a monomer (Mr 25 kD). The non-active gf-3.8 protein is synthetised in transgenic tobacco plants only under control of the CaMV 35S promoter. Consequently, among two germin isoforms, only the gf-2.8 protein seems to be regulated by hormonal, salt and heavy metal factors. The gf-2.8 oxalate oxidase activity could be then involved in general stress-induced signalling in plant.

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Effect of dietary chromium and nicotinic or ascorbic acids on performance and serum cholesterol and triglyceride content of growing-finishing pigs

80%

Grela E. R., Kondek E., Czech A.

Annals of Animal Science. Supplement

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2002

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nr 2

5

Badanie i ocena sanitarno-higieniczna piwa butelkowego

71%

Wierzchowski J., Czarnowska W., Severin M.

Roczniki Państwowego Zakładu Higieny

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1957

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tom 08

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nr 6

Ograniczanie wyników

2 Roczniki Państwowego Zakładu Higieny

1 Acta Physiologiae Plantarum

1 Annals of Animal Science. Supplement

1 Journal of Physiology and Pharmacology

1 Bieron K.

1 Czarnowska W.

1 Czech A.

1 Grela E. R.

1 Gryglewski R. J.

1 Kondek E.

1 Kostka-Trabka E.

1 Markuze Z.

1 Nowakowska J.

1 Severin M.

1 Swies J.

1 Wierzchowski J.

1 2002

2 1998

1 1957

1 1956

Thrombolytic and antiplatelet action of Xanthinol nicotinate [Sadamin]: possible mechanisms

Chemiczna metoda oznaczania kwasu nikotynowego w produktach spożywczych

Gene expression and oxalate oxidase activity of two germin isoforms induced by stress

Effect of dietary chromium and nicotinic or ascorbic acids on performance and serum cholesterol and triglyceride content of growing-finishing pigs

Badanie i ocena sanitarno-higieniczna piwa butelkowego