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Effect of the iron chelate FeEDDHA on Staphylea pinnata shoot multiplication and photosynthetic pigment concentration

100%

Szewczyk-Taranek B., Pawlowska B., Prokopiuk B., Moczulska K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

2

Rooting of a trumpet creeper (Campsis radicans (L.) Seem.) microshoots in presence of auxins

100%

Parzymies M., Dabski M., Pogorzelec M., Kozak D., Durlak W., Dudkiewicz M.

Acta Scientiarum Polonorum. Hortorum Cultus

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2014

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tom 13

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nr 5

A trumpet creeper (Campsis radicans) is an attractive vine propagated vegetatively through cuttings. So far, there is very little available data on propagation of this beautiful species in tissue culture. There was a research conducted in order to estimate the possibility to obtain rooted Campsis radicans plants that had been cultivated in tissue culture. The plant material were shoots obtained by multiplication on Murashige and Skoog [1962] (MS) medium which were put in fresh media supplemented with auxins: IAA (indoleacetic acid), IBA (indolebutyric acid) or NAA (naphthaleneacetic acid). The shoots were rooted in vitro or transplanted into soil (peat + perlite 1 : 1 w/v). It was noted that Campsis radicans is a very difficult plant to root in tissue culture. No rooting was obtained in vitro. Use of a stimulating passage with a hormone free medium or the ones containing IAA or IBA in concentrations of 2.5–5 mg·dm-3 and rooting shoots directly in soil allowed to obtain 100% of well rooted plants.

3

Shoot multiplication and plant regeneration of guava [Psidium guajava L.] from nodal explants on in vitro raised plantlets

67%

Rai M. K., Jaiswal V. S., Jaiswal U.

Journal of Fruit and Ornamental Plant Research

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2009

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tom 17

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nr 1

29-38

A micropropagation method is described for guava (Psidium guajava L.) using nodal expiants from somatic embryo-derived young and aseptic plantlets. Multiple shoots were induced from axillary buds on MS medium containing different concentrations of N6-benzylaminopurine (BAP), either alone or in combination with kinetin (Kn), indole-3-acetic acid (1AA) or a-naphthalene acetic acid (NAA). Medium containing 1 mg l-1 BAP was the most effective for shoot multiplication. In vitro regenerated shoots developed roots either on MS medium alone or on MS medium supplemented with indole-3-butyric acid (1BA). The rooted plantlets were successfully acclimatized.

4

The impact of three bacteria isolated from contaminated plant cultures on in vitro multiplication and rooting of microshoots of four ornamental plants

67%

Zawadzka M., Trzcinski P., Nowak K., Orlikowska T.

Journal of Horticultural Research

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2013

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tom 21

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nr 2

5

Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants

59%

Hanus-Fajerska E., Wiszniewska A., Czaicki P.

Acta Agrobotanica

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2012

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tom 65

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nr 1

In this study, an attempt was made to investigate in vitro morphogenetic competence of three shrub species from the Thymelaeaceae family. The studied plant material originated from Russia, Greece and China, and the effectiveness of in vitro shoot formation and rhizogenesis of Daphne caucasica, D. jasminea, and D. tangutica was verified. The multiplication coefficient was compared for different propagation media. Medium composed of WPM mineral salts, MS microelements and a set of vitamins, supplemented with 1.0 mg dm-3 2iP, 0.1 mg dm-3 NAA, and 0.65 g dm-3 calcium gluconate, was appropriate for micropropagation of the tested genotypes. Shoot propagation in medium containing B5 vitamins and microelements was not as effective as on WPM/MS medium. The rooting phase, especially in D. tangutica, needs further optimization in order to reduce the costs associated with acclimatization of microplantlets obtained to in vivo conditions. After stabilization, the plants were successfully cultivated under greenhouse conditions.

6

In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems

59%

Kaviani B., Bahari B.

Acta Scientiarum Polonorum. Hortorum Cultus

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2019

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tom 18

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nr 4

A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.

Ograniczanie wyników

Effect of the iron chelate FeEDDHA on Staphylea pinnata shoot multiplication and photosynthetic pigment concentration

Rooting of a trumpet creeper (Campsis radicans (L.) Seem.) microshoots in presence of auxins

Shoot multiplication and plant regeneration of guava [Psidium guajava L.] from nodal explants on in vitro raised plantlets

The impact of three bacteria isolated from contaminated plant cultures on in vitro multiplication and rooting of microshoots of four ornamental plants

Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants

In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems