One of the latest and most effective disinfectants is the gas form of hydrogen peroxide (H₂O₂). The disinfection process runs in closed air circulation minimizing the threat to operating personnel. The advantage of the system is a broad spectrum of biocidal effects as well as the fact that the gas form of the biocidal substance has no impact on materials placed in the disinfected area (inter alia metals, plastics, installations, construction materials) and also guarantees the entire fill up of the gassed room. A comparison was made of “dry” and ”wet” disinfection methods of gas. For comparison purposes generators from Bioquell and Steris companies were chosen. Both devices are low-temperature mobile disinfection systems using a disinfecting agent in the form of 35% perhydrol and operating in a cubic capacity of up to 500 m³. In order to keep identical conditions during both processes, the comparison was made in the same room under similar physical conditions. Additionally, for the “wet” system process was carried out in a room of cubic capacity of 250 m³. To confirm the effectiveness of the decontamination processes microbiological tests using Geobacillus stearothermophilus bacteria (placed on metal discs and put in Tayvek bags) were applied. It was found that in both cases the decontamination systems demonstrated high biocidal efficiency. During the comparison of both methods it was noticed that in the case of the generator from Bioquell UK Company the optimization of the process intended to maintain a micro-condensation layer is possible only during decontamination of rooms of cubic capacities up to 250 m³. Above that cubic capacity the generator does not optimize the process. This means that the decontamination process conducted in such a manner is uncontrolled and may cause condensation of the disinfection agent on surfaces of expensive laboratory equipment. After the process some paint detachments were found in laboratories.
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