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Industrialization of sauerkraut production in Poland will need to solve the problem associated with disposing of highly acidic waste effluents. Late sauerkraut brine present the greatest problem with respect to treatment because of their high BOD and low pH. Degradation rate of sauerkraut brine by Kluyveromyces marxianus yeast - depends on concentration of the brine in the medium. The removal of lactic acid in shake flask experiments varied from 98.95 to 56.75 % after 48 h at 30°C. The immobilization of yeast in sodium alginate improved the lactic acid degradation rate and allow to treat the very acid brine directly. Lactic acid was fully used in diluted brine in 24 h and the content in undiluted brine was reduced by 90.17 to 81.2 to % in the following runs after 48 h.
The amounts of biogenic amines (putrescine, histamine, cadaverine, tyramine, spermidine, and spermine), lactic acid, pH, and number of Enterobacteriaceae, Escherichia coli, and coagulase producing Staphylococci were determined in healthy turkeys and in the fillets of turkey breasts with the signs of ascites and bursitis. The examination of the amines was performed by high performance liquid chromatography; the amount of lactic acid was determined by capillary isotachophoresis method; pH value and microbiological indicators - by standardised methods. All determinations were done after 24, 72, and 120 h after slaughter of the turkeys. The fillets of the breasts were stored in the refrigerator at 4°C. The significant differences in the total amounts of biogenic amines in carcasses of healthy turkeys and carcasses of turkeys with substantial lesions of bursitis and medium or substantial lesions of ascites were determined within 5 d after slaughter (P<0.00l). The amounts of biogenic amines in the breast fillets of turkeys with low lesions of ascites and bursitis were very similar to that of the control group during the whole period of maturation. The average amount of lactic acid throughout 5 d after slaughter increased only in the breast fillets of healthy turkey (maximum value was 1.032 mg/100 g) and insignificantly increased in the carcasses with low lesions of ascites and bursitis (maximum value was 0.983 mg/100 g). The results of investigation indicate the breast fillets of turkeys with medium and substantial lesions of ascites and substantial lesions of bursitis are not fit for human consumption. However, in the cases of low lesions of ascites and low and medium lesions of bursitis, the fillets are not fit for maturation, but could be thermally processed in the period of 24 h after slaughter.
The investigations were conducted on 60 Holstein-Friesian dairy cows (at age 3 year and weight 590 kg) kept in tie-stall barn. The animals were divided into 3 groups of 20 heads each. The control group (K) was fed diets without probiotics, group (EM) -- was fed diet with the addition of EM probiotic (dose of 150 ml ⋅ t-1 TMR) and group (T) -- was fed diet with the addition of ToyoCerin probiotic (dose of 0.2 kg ⋅ t-1 TMR). The volume of 2--10 cm$^3$ saliva was collected from each animal in which the following parameters were determined: number of lactic acid rods from the Lactobacillus genus, number of rods capable of producing hydrogen peroxide. For purpose of precise diagnostics, lactic acid rods were identified on the basis of biochemical traits employing API 50 CHL (BioMérieux), while those manufacturing H2O2 were additionally tested using PCR method. The occurrence of Lactobacillus spp. rods was confirmed in all the examined individuals and in each and every experimental combination. Lactobacillus spp. rods capable of produce hydrogen peroxide were isolated in 17 cows in group K, in 3 individuals in group EM and in 13 animals in group T. EM probiotic strongly significantly restrict the development of Lactobacillus spp. strains are capable to produce hydrogen peroxide.
The aim of the study was to investigate the effect of selected technological additives (NaCL, sodium lactate and lactic acid) on shelf life of ground meat. It was found that all the three substances cause reduced microbial growth. Dynamics of action in case of each of these substances vary and depend on their concentrations. A limitation in the feasible amount of applied additives may be their effect on sensory attributes of the product. There is a marked, advantageous interaction of the preserving activity of these substances. Using recorded results it is possible to optimize the composition of the mixture of analysed additives.
The studies aimed at determining the influence of lactic acid on Salmonella spp. during storage of turkey carcasses’ samples for 2, 4 and 6 days. The initial average contamination of turkey carcasses’ elements with Salmonella spp. was 2.4 · 10³ bacteria. Following the immersion in water in average 4.3 · 10² Salmonella spp. cfu was recovered and that number was assumed as the inoculum. The number of Salmonella spp. decreases during storage of turkey carcasses’ samples in the refrigerator at 4℃. Compared to elements of carcasses immersed in sterile water the largest reduction, by two logarithmic cycles was recorded after 2 days of storage of samples treated with 1% lactic acid. In case of the other variants of the experiment when 1% solution of lactic acid was applied S. Enteritidis grew in numbers within the same logarithmic range. Compared to the samples immersed in sterile water, 2% lactic acid caused reduction in the number of Salmonella spp. on elements of poultry carcasses by one logarithmic cycle both immediately after contamination and after 2 and 6 days of storage; unfortunately after 4 days of storage S. Enteritidis grew in numbers that were within the same logarithmic range. During storage of the turkey samples tested at 4℃ for 2, 4 and 6 days, the numbers of Salmonella spp. decreased. That decrease compared to samples immersed in sterile water was the largest after 2 days of storage after application of 1% lactic acid.
Lactic acid has been known as one of compounds to cause cellular harm in waterlogged tissues through the process of cytoplasmic acidosis. The effects of lactic acid on α-amylase activity and phytic acid (myo-inositol 1,2,3,4,5,6-hexaphosphate) content using an assay for high phosphate in the germination stage of rice were evaluated. It is showed that lactic acid inhibited rice germination at every treated dose. The reduction of α-amylase content attributed to lactic acid at 24 h after germination of rice seeds was observed. The findings highlighted the effects of lactic acid on α-amylase activity and phytic acid content and suggested that this compound may play a potent role as a germination regulator in rice.
The ethyl acetate fractions of aqueous extracts from two submerged macrophytes Potamogeton malaianus and Potamogeton maackianus were analyzed by gas chromatograph-mass spectrometry (GC-MS). The allelopathic activities and joint effects of the main components in ethyl acetate fractions on Microcystis aeruginosa were also determined. The results indicated that primary compositions in the ethyl acetate fractions were fatty acids, phenolic acids and hydroxy fatty acids that possessed antialgal activities. The joint effect assay for palmitic acid, benzoic acid and lactic acid showed that the additional effects were observed in the mixed organic acid, namely, the inhibitory effects of mixture groups were stronger than that of each compound alone on the growth of M. aeruginosa.
The study was conducted on 300 turkey cocks type BUT-9, grown under optimal conditions for this kind of poultry. The feed was a typical commercial full-dose mix for turkeys. The birds were divided into 5 experimental groups. From the 3rd week of their life, they were given preparations with their drinking water. Group I was the control group, while drinking water for the test groups was enriched as follows: lactic acid (0.4%) for group II, CuSO4 in the amount of 30 mg Cu·dm-3 H2O for group III, CuSO4 (30 mg Cu-dm-3) and 0.4% of lactic acid for group IV and CuSO4 in the amount of 50 mg Cu·dm-3 for group V. Application of the preparations was terminated after 3 days, when symptoms of poisoning were observed in turkey cocks of groups II and IV, alongside increased mortality rate. Samples of drinking water were taken for analyses, and from each group 10 birds were selected for slaughter to take tissue samples (breast and leg muscles). The objective of the experiments was to determine the concentration of Ca, Mg, Zn, Cu and Fe in the consumable tissues of turkey cocks. The study showed that the highest rate of mortality of turkey cocks was observed in the group that was given lactic acid with their drinking water, and these turkeys had increased levels of Ca, Cu, Zn and Fe. In none of the groups, the content of Zn in the breast muscle exceeded 20 mg kg-1, while the concentration of copper in all experimental groups (except the control) was above the level of 10 mg kg-1. Increased doses of copper caused an increase in the concentration of magnesium in the leg muscles, while a combination of copper supplementation with lactic acid resulted in increased accumulation of Cu in both the breast and leg muscles.
This study aimed to determine the antimicrobial activities of sumac (Rhus coriaria L.) water extract (8.0 %, wt/vol), thyme (Thymus vulgaris L.) water (commercial hydrosol) and ½ thyme water (1:1, commercial hydrosol / distilled water, vol/vol) in vitro in comparison with lactic acid (1.0 %, vol/vol), against the foodborne pathogenic bacteria Escherichia coli O157:H7, Listeria monocytogenes 4b, Staphylococcus aureus and Yersinia enterocolitica O3. The test microorganisms were inoculated to the treatment solution tubes. All the inoculated tubes were kept at 20 ± 2°C for 60 minutes. The numbers of the four test bacteria decreased to an uncountable level (<1 cfu/ml) in 1 min in the thyme water. The counts of all the pathogens, with the exception of L. monocytogenes 4b, were lower than the countable level after 1 min in the lactic acid. Both lactic acid and ½ thyme water reduced the test bacteria counts to the uncountable level in 10 min. In the sumac extract, the reduction time periods were 10 min for E. coli O157:H7, 30 min for Staph. aureus and 60 min for L. monocytogenes 4b and Y. enterocolitica O3. However, when enriched after treatment, E. coli O157:H7 and Staph. aureus were grown in lactic acid, S. aureus was grown in ½ thyme water and E. coli O157:H7, Staph. aureus, and L. monocytogenes 4b were grown in sumac extract. Thyme water had the strongest antibacterial activity against both the Gram negative and the Gram positive bacteria tested, followed by lactic acid, ½ thyme water and sumac extract.
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