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Background. The aquaculture rainbow trout may be a valuable source of long chain n-3 polyunsaturated fatty acid (LC n-3 PUFA). In the retail these fish are mainly present as a whole or gutted. The present study was aimed at comparing changes occurring in lipids of whole and gutted rainbow trout stored in ice. Materials and Methods. The analysis were performed after 0, 3, 7, and 14 days of storage in ice at 2°C and the following assays were carried out: proximate composition; lipid composition high pressure liquid chromatography (HPLC); fatty acid composition gas chromatography /mass spectrometry (GC/MS) by direct tissue saponification; contents of lipids extracted, using the Bligh-Dyer technique; UV-VIS, IR, and fluorescence lipid spectra; peroxide value (PV); anisidine value (AsV); and acid value (AC). Results. Gutting prior to storage made it possible to extend the sensory shelf-life by about 2-3 days and affected the quantitative fatty acid composition and oxidation level during storage in ice. The rainbow trout lipids are resistant to oxidation; oxidation product decomposition rather than lipid oxidation proceeds during storage, the decomposition being more intensive in whole than in gutted fish. It is only when the fish lose their eating quality (2 weeks) that a small increase in the level of oxidation occurs, accompanied by an about 15% loss of n-3 PUFA and a 20% loss of DHA, but only in the whole fish. Conclusion. Gutting rainbow trout prior to storage in ice is appropriate by the extending the shelf-life by about 2-3 days and keeping stable amount of n-3 PUFA during 2 weeks of storage.
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Antarctic benthic fauna in the global climate change

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An important question stated in the presented paper is: Could rotifers be the indicators of climate change in the Antarctic environments? Rotifers are one of the most important components of the invertebrate fauna of the Antarctic freshwater and terrestrial ecosystems. However, investigations of their distribution, diversity and an ecological role in the environment have been widely neglected, mainly due to the methodological and taxonomical difficulties. During the investigations 176 species of rotifers were found, including the endemic and cosmopolitan species. Their existence is directly proportional to microvariations of humidity factor. Scientists still have poor knowledge about the occurrence of rotifer species in recently studied habitats e.g. cryoconite holes, nunataks or soil. Probably rotifers could be the indicators of climate change in the Antarctic freshwater and terrestrial ecosystems but more investigations and monitoring studies are needed.
The aim of this study was to work out a recipe for fruit ice with addition of soy protein isolates. The influence of sorts and dose of hydrocolloids on the physicochemical and sensoric properties of fruit soy ice was studied. The soy and whey proteins were compared as ice stabilizer. The investigations were performed to work out recipes for soy ice containing similar contents of protein as that of based on milk or containing higher contents of soy protein up to 6.25 g in a portion of ice. The quality of products was determined on the basis of the sensory evaluation, the apparent viscosity and density of ice mixture and on the basis of overrun and melting time of the ice.
Winter survival of cereals and grasses depends mainly on plant resistance to low temperature and to snow mould fungi. To persist winter plants have to be tolerant to different kind of stresses: abiotic such as low temperature, long-term snow and ice cover, freeze-induced plant desiccation or frequent freezing and thawing, and biotic - many species of snow mould fungi. During the cold acclimation, cereals and grasses become more resistant to both stresses: cold and snow mould. Earlier seeded plants with a greater number of crowns are more resistant to snow mould. Infection caused by snow mould induces a complex plant response, including such processes as the synthesis of PR (pathogenesis-related) proteins (chitinase and β-1,3-glucanas), production of active oxygen species (AOS), synthesis of phenolics, phyotalexins, accumulation of callosis and soluble carbohydrates, and a decrease of water potential. In the paper the most common defence mechanisms against snow mould pathogens are discussed.
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