The content of glycoalkaloids was determined during vegetation of potato tubers of very early varieties (Aster, Drop, Malwa, Orlik) cultivated in 1993 on the fields of the Experimental Station for Variety Evaluation at Węgrzce near Cracow. The content of glycoalkaloids ranged from 0.7 to 17.8 mg/100 g, wet basis, at the average content of 5.6 mg/100 g and the smallest content of 1.3 mg/100 g in potato tubers harvested in the latter part of August. In potatoes stored at different conditions, the content of glycoalkaloids statistically increased. After 6 months, tubers stored at 10°C contained less glycoalkaloids than those stored at 4°C.