The study presents the measuring position for determination of the dynamical behavior of vegetables and fruit. The main element of the device is an instrumented rigid physical pendulum made of carbon fi ber. The pendulum arm was developed as a double arch connected with the external links. This construction enables attaining the desirable value of the quotient of the mass moment of inertia of the pendulum arm with a fruit and the arm without it as well as appropriate rigidity. The studies with the pendulum device application simulated the apple drop from a required height. The tests measuring pendulum rotation angle and fruit response force in time facilitated determination of the force – displacement correlation at impact event. The obtained displacement and impact force courses in time were presented graphically.
This paper reviews the results of an extensive monitoring study, spanning 20-years of observation from three medical centres in Olsztyn on the dynamics and species diversity of fungi most frequently colonizing the respiratory and digestive systems of humans. The experimental materials were swabs and specimens from the gastrointestinal tract, swabs from the oral cavity and pharynx, as well as sputum and bronchial fluid from the respiratory system. The biological material was subjected to routine mycological diagnostics, taxonomic determination and identification. In total, 41 species of yeast and yeast-like fungi were isolated, including 34 from the respiratory and 25 from the digestive system. In the last decade, a significant increase has been noticed in the counts of fungi, especially the gastrointestinal tract, reported from people. As many as 18 species were isolated from both systems – they were predominated by fungi of the genus Candida and their perfect forms. Worthy of notice are also frequent isolations of yeast (Saccharomyces spp.) and detection of an endemic species, Paracoccidioides brasiliensis, in the respiratory system, and of the sexual stages of Rhodosporidium diobovatum and Rhodosporidium kratochvilovae from the gastrointestinal tract.
Acrylamide and its analogues have been widely used since the last century for various chemical and environmental applications and can be formed by heating of biological material derived from plant tissues. This compound, identified previously as a potential industrial hazard, has now been found in many cooked foods.
A rapid, accurate, simple, and reproducible high performance liquid chromatographic (HPLC) method for the determination of oxytetracycline hydrochloride (OTC) in porcine plasma has been developed and validated. The drug and the standard were eluted from 5 um Omni Spher (Varian) C18 column (250 mm x 4.6 mm I.D.) at room temperature. The mobile phase was composed of ACN-MeOH-(HCOO)₂ (17.5:17.5:65 v/v/v) (pH adjusted to 2). A flow rate was 1.4 mL/min. The effluent was monitored using a UV-VIS detector set at 360 nm. The retention time of OTC was about 3 min. The suggested technique is characterised by superior performance parameters: linearity R² = 0.9999, recovery = 92.50%, repeatability RSD ≤ 1.39%. These results demonstrate the validity of the HPLC method for the analysis of OTC HCL.
A reverse-phase HPLC spectrofluorometric method was developed for routine, specific and fast analysis of glutathione (GSH) in biological materials based on modified O-opthaldialdehyde derealization procedures. The developed method allowed the determination of glutathione in yeast biomass, bile and plasma samples from goats. The limit of detection was estimated to be 8 µМ for yeast biomass at a signal to&noisc ratio of 1 and was further extended to signal ratio of 2, which allowed the detection of lower amounts of GSH in the bile and plasma up to 10 ng/cm3 The methods ol GSH extraction from biological materials, derivatization procedures and separation by HPLC method were reported.
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