The objective of the study carried out during 2004-2007 was to evaluate the influence of three types of substrates (peat, sawdust and cocoa husk) on yield, quality and chemical composition of highbushblueberries cv. 'Sierra'. On 4-year average, the lowest yield per a bush (0.89 kg) was obtained from bushes grown in cocoa husk substrate and these berries had the lowest weight of 100 fruit (64 g) and were the smallest. On the other hand, they had the highest firmness, measured both vertically (512 G mm-1) and horizontally (275 G mm-1). The highest soluble solids content (14.45%) and titratable acidity (1.02 g of citric acid equivalents 100 g-1 ) were found in fruit harvested from bushes growing in peat. The substrates did not affect significantly total sugar (11.98-12.30 g 100 g-1) and vitamin C contents (26.0-27.9 mg 100 g-1). The nitrates content in blueberries was low, ranging from 17.5 (peat) to 34.7 mg kg-1 (sawdust) whereas the content of nitrites varied from 0.85 (peat) to 1.10 mg kg-1 (sawdust). Blueberries obtained from peat- and cocoa husk- bedded bushes had significantly higher total phenolics content (231.03 mg and 222.29 mg per 100 g-1, respectively) compared to these grown in sawdust (181.74 mg 100 g-1). Similarly, the berries originating from peat- and cocoa husk- grown plants showed higher total anthocyanin content (144.55 mg 100 g-1 and 146.73 mg 100 g-1, respectively) than fruit collected from sawdust-bedded bushes (120.92 mg 100 g-1). Taking into account the yield alone, the best effects were obtained from 'Sierra' bushes grown in sawdust. However, regarding chemical composition, the berries from bushes grown in peat and cocoa husk were richest in nutritional components and, especially, in phenolic compounds.