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Association of CAST and RYR1 genes polymorphism with carcass and meat quality in crossbreed pigs with a share of Pietrain breed. The aim of this study was to determine the effect of the calpastatin (CAST/TaqI) and ryanodine receptor (RYR1) genes polymorphism on carcass and meat quality traits in Pietrain crossbred pigs. The polymorphism in CAST and RYR1 genes was detected using the PCR-RFLP (Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments) method. Two alleles of CAST gene were identified–A(0.34) and B (0.66) and three genotypes–AA(0.21), AB (0.25) and BB (0.54). In relation to carcass and pork quality, no statistically significant differences were found between the CT and CC genotypes of RYR1 gene as well as between AA, AB and BB genotypes of CASTgene. In addition, no significant interaction was found between CAST/TaqI × RYR1 genotypes and all the analyzed carcass and meat quality traits.
The aim of the experiment was to determine the RYR1 genotype and to analyse the distribution of osteochondrosis (OC) in wild boars from different regions of Lithuania. Hair and bone samples were collected from wild boars of various sexes and ages that had been shot during hunting season. Genomic DNA was extracted from hair roots. The RYR1 gene was analysed with the PCR-RFLP method, using specific primers and restriction enzymes Alw211 and Hin61. Only one C allele (q = 1.00) and CC genotype of the RYR1 gene were identified. Thus 138 genotyped wild boars were stress-resistant with both dominant alleles intact (no mutation). OC was measured by the cut surface of the distal femur and humerus. The prevalence of OC among the 34 wild boars tested was 20.6%. 5 animals (14.7%) had OC lesions in the hind legs and 2 animals (5.9%) had them in the front legs. OC affected leg joints more often in adult females (third-years and older), but this dependence was not significant (p > 0.05).
The aim of this study was to characterize the polymorphism of genes RYR1, LEP, GH, MYOG, MYF5 and GDF8 in wild boar population inhabiting North-East of Poland. The above genes are considered candidate genes for carcass quality traits in domestic pigs. Ninety animals were tested. Genes RYR1, LEP and MYF5 appeared to be monomorphic, whereas genes MYOG, GH and GDF8 showed polymorphism similar to that observed in European pig breeds. However, no PCR product was obtained for several animals when MYOG and GDF8 gene fragments were amplified, suggesting the difference between wild boars tested and domestic pig in sequence of the gene regions where primers annealed. Moreover,this suggests a possibility of identification of new variants of these genes in wild boar.
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