A micropropagation method is described for guava (Psidium guajava L.) using nodal expiants from somatic embryo-derived young and aseptic plantlets. Multiple shoots were induced from axillary buds on MS medium containing different concentrations of N6-benzylaminopurine (BAP), either alone or in combination with kinetin (Kn), indole-3-acetic acid (1AA) or a-naphthalene acetic acid (NAA). Medium containing 1 mg l-1 BAP was the most effective for shoot multiplication. In vitro regenerated shoots developed roots either on MS medium alone or on MS medium supplemented with indole-3-butyric acid (1BA). The rooted plantlets were successfully acclimatized.