A protocol has been developed for an efficient tissue culture cycle (callus induction, callus growth, plant regeneration and root induction) for date palm 'Khalas' using various plant growth regulators (PGR) in both liquid and solidified MS medium. The shoot explants showed high callus induction potential on MS medium supplemented with 45.24 ^M 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 54.21 ^M 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). The highest frequency of shoot regeneration (85%) and number of shoots per an explant (5.6) were obtained on solid MS medium supplemented with 7.84 ^M N6-benzylaminopurine (BAP). Rooting (87.34%) was high on solid MS medium supplemented with 24.6 ^M indole-3-butyric acid (IBA). However, the root length was higher in MS liquid medium. This protocol will be useful for rapid, large-scale propagation of 'Khalas' date palm.