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The aim of the experiment was an attempt at answering the question if an excessive supply of iron has an influence on the content of GAG in the liver. The experiment was carried out using Chinchilla rabbits (3.2±0.1 kg b.w.). The animals were divided into 3 groups (7 rabbits in each group) and one control group (5 rabbits). The animals in each group except the control group received a pharmaceutical form of iron (intramuscular injection of Jectofer 15 mg/kg b.w./day) for 10, 90 and 120 days, the control group received 0.9% NaCl in an identical manner. Respectively after 10,90 and 120 days the animals were put to sleep (penthobarbital sodium), and after that GAG were isolated from their livers. Glycosaminoglicans were isolated using Svejcer method as modified by Wosicki. The GAG quantity was determined by Bitter and Muiry method by means of glucuronic acid contained in it. After 10 days of Jectofer administration we noticed a 15% decrease of GAG quantity in rabbits’ livers in contrast with the control group; on the other hand after 90 and 120 days of the experiment we noticed increased GAG quantity in the investigated livers by 33% and 62% respectively compared with controls. The obtained results permit us to ascertain that an excessive supply of iron in rats leads to restructuring in the connective tissue of the liver, the noticed changes of GAG quantity were the proof of this One of the consequences may be the hepatic in jury. Although our experiment cannot give a sufficient answer as to how and why excessive accumulation of iron causes the increase of GAG quantity in rabbits’ livers after a long lasting exposition on high doses of Jectofer, with great probability we can say that all those changes are the result of the disturbance of oxidative homeostasis in the liver.
The purpose of this experiment was to estimate the most effective doses of TFX, used in injection and in bath, for the stimulation of the activity of lymphocytes in common carp The study was performed on 9-month-old carps obtained after spring catches (in March). The fish were divided into 10 groups. Fish in groups I-IV were given TFX by intramuscular injection at doses of 20, 10, 0.5, and 0.2 mg/fish. Fish in groups VI-IX were bathed for 24, 48, and 96 hours in TFX at concentrations of 200, 100, 50, and 20 mg/l of water. Groups V and X were control. Blood samples were taken immediately after the administration of TFX in bath and after 2, 7, 14, and 28 days in groups I-IV and VIII-IX. The following parameters were determined: the number of leukocytes and lymphocytes in 1 μl of blood, the percentage of ANAE+ and E-rosette-forming cells, and the proliferative activity of lymphocyte cell cultures stimulated with Con A. Results: TFX at doses of 100, 200 mg/l of water and 10, 20 mg/fish administered by injection showed a suppressive effect on the parameters investigated. TFX at doses of 50, 20 mg/l of water and 0.5, 0.2 mg/fish caused an increase in the number of leukocytes and lymphocytes, a higher percentage of ANAE+ cells and rosette-forming cells, as well as a multiple increase in the proliferative activity of lymphocytes stimulated with Con A. The highest values were obtained after the administration of TFX in a 96-hour bath at a concentration of 50 mg/l of water.
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