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In this study, the antimicrobial properties of essential oils obtained from Coriandrum sativum, Foeniculum vulgare Miller, Salvia triloba, Laurus nobilis L., Citrus limon and Origanum smyrnaeum L. were investigated. A total of eight microbial organisms belonging to six species of bacteria, namely Salmonella typhimurium, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli and Enterobacter aerogenes, as well as two fungi, Candida albicans and Aspergillus niger, were studied using a disc-diffusion and agar dilution (minimal inhibition concentration) method. The antimicrobial activity of essential oils obtained from the six plants turned out to be more effective in the case of bacteria than against fungi. The antimicrobial activity against Gram-positive bacteria was more pronounced than against Gram-negative ones. All the investigated plants are known as having healing properties and are used to treat various diseases. The essential oils obtained from L. nobilis and O. smyrnaeum showed the highest antifungal activity against C. albicans and A. niger, while the essential oils obtained from F. vulgare showed the highest antimicrobial activity against P. aeruginosa and E. coli. On the other hand, the essential oils obtained from O. smyrnaeum showed stronger antibacterial activity in the case of E. aerogenes and S. aureus, but were not equally effective against E. coli. The other crude essential oils showed varied levels of antibacterial and antifungal activity. The minimal inhibition concentrations (MIC ) of the essential oils obtained from O. smyrnaeum and of those obtained from L. nobilis ranged from 1.17 to 4.71 mg/ml, and 2.4 to 19.2 mg/ml, respectively.
Supercritical fluid chromatographic (SFC) separations of a-tocopheryl acetate, α-, ß-, y- and -tocopherol have been achieved on a Spherisorb S3 ODS2 column in about 15 minutes. Separation efficiency of this method has been optimised with respect to the effect of pressure, percent of methanol in the mobile phase, temperature and flow rate, resulting in theoretical plate numbers (N) varying from 63000 to 90000 per metre. Linear correlations have been found between log k' (retention factor) and fluid density for all of the tested compounds, and a van't Hoff plot yielded linear correlation between log k' and T-1 at constant mobile phase density. Linearity and repeatability were found to be acceptable with detection limits from approximately 20 ng for α-, ß-, y- and ő-tocopherol to 10 ng for a-tocopheryl acetate at a signal to noise ratio = 3. Results obtained by use of the developed SFC method to determination of tocopherols in plant oils obtained by SFE (supercritical fluid extraction) and by aqueous enzymatic processing are presented.
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Tocopherol content in edible plant oils

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Edible vegetable oils were analysed for tocopherols by reversed-phase HPLC. These included refined corn, soybean, sunflower, rapeseed, grapeseed, and peanut oils, and cold-pressed extra-virgin olive, linseed, rapeseed, and sunflower oils. Total measured tocopherol concentrations varied in the range from 121 to 829 mg/kg. The contents of individual α-, (β+γ)-, and δ-tocopherols show a great diversity depending on the kind of oil. Moreover, the effect of photooxidation on the tocopherol content was studied in cold-pressed sunflower and rapeseed oils. These studies revealed only a minor decomposition of tocopherols under visible light.
This study was done to assess the antifungal effect of some biocontrol agents effective microorganisms (EMs1), Pseudomonas fluorescences, and Bacillus pumilus, titanium dioxide (TiO2) nanoparticles, black cumin and wheat germ oils as well as the recommended fungicide (flutolanil) against root rot of tomato. Moreover, gas chromatography-mass spectrometry (GC-MS) examination was completed to identify the bioactive compounds in plant oils (dark cumin and wheat germ). Also the impact of these medicines on some biochemical and growth parameters of tomato was examined. Flutolanil was the best treatment followed by dark cumin, TiO2, EMs1, Pseudomonas fluorescens, Bacillus pumilus and wheat germ oil, individually in both test seasons. The outcomes demonstrated a marked increase in each biochemical character (chlorophyll substance, peroxidase and polyphenoloxidase) and plant development (height and fresh and dry weight) under all the tried treatments in comparison to the controls.
The quantity of the crop plant pests have to be under control. A defense against pest organisms with toxic chemicals has been the prevailing pest control strategy for over 50 years. However, plants were found to possess insecticidal properties. Several essential oils act as antifeedants, deterrents and repellents. Essential oils are products of the secondary metabolism in plants. They are mixtures of simple and volatile compounds. One of the example is lavender oil, which repels Ceutorhynchus sp. - the most important pests of Brassica napus var. oleifera plants. The same effect was observed in the case when linalool, linalyl acetate (main components of lavender oil) and lavandulol were applied [Duda, Dubert 2008a, 2008b]. Thymol, the major constituent of thyme oil, is also a deterrent for the lepidopteran pest - Plutella xylostella L. [Akhtar, Isman 2004].
The aim of the study was to determine contents of phytosterol and oxyphytosterols in soybean oil and soybean oil with the addition of sea buckthorn seed oil after thermal processing, and to assay the oxidative stability of the oils. The analysis verified that the thermal processing of soybean oil at 120°C induced a decrease in total phytosterols (phytosterols concentration was reduced by 5.2 to 21.0%) and formation of the following phytosterol oxidation products: 7-ketositosterol, 5α,6α-epoxysitosterol, 7-ketocampesterol, 5α,6α-epoxycampesterol, 7-ketostigmasterol, and 5α,6α-epoxystigmasterol. The content of phytosterol oxidation products (POPs) increased with the higher sea buckthorn seed oil addition to soybean oil (from 1.2 mg/100 g in soybean oil with 1% addition of sea buckthorn oil to 11.4 mg/100 g in soybean oil with 5% addition of sea buckthorn oil) during Rancimat test.
The present work was planned to determine the median lethal doses (LD₅₀) and to evaluate the haematological and biochemical changes, and histopathological effects of the essential oils from eucalyptus Eucalyptus globules L. and clove Eugenia caryophyllus on liver and kidney of albino rats. The LD₅₀ was 2,334.4 and 3,597.5 mg/kg b.w. of eucalyptus and clove oils, respectively. Obtained data revealed that 1/10 LD₅₀ of both tested oils resulted in a significant increase in WBC counts and produced a significant decrease in haemoglobin concentration and platelets count at 5th and 10th doses, as well as RBC counts (-17.1 and -9.4% below normal level) at 10th dose. The activities of serum GOT and GPT enzymes were a significant increase at 5th and 10th doses in treated rats by both tested oils. While two essential oils had mild effect on kidney function, these oils produced a significant increase in creatinine and urea concentration at the 10th dose. Histopathological studies on liver and kidney revealed that both essential oils caused relatively moderate pathological changes in the liver as congestion of the blood vessels in the portal area associated with inflammatory infiltration. Also, two tested oils induced desquamation of the epithelial cells of the renal tubules.
Przebadano skład kwasów tłuszczowych w 27 próbkach olejów roślinnych, pochodzących od 13 producentów i zakupionych w handlu detalicznym w latach 1996-1999. Stwierdzono, że badane oleje roślinne zawierały stosunkowo mało nasyconych kwasów tłuszczowych (od 5,8 do 18,5%), natomiast dużo mono- (do 79,1%) i polienowych (do 77,0%). Izomery trans występowały w badanych próbkach w ilościach śladowych.
Phytosterols are one class of food constituent with serum cholesterol-lowering properties by inhibiting cholesterol absorption. These reasons led to an increasing availability of phytosterol-enriched foods. The content of phytosterols in rapeseeds ranged from 4.7 mg/g to 6.3 mg/g of seeds depends on variety. After pressed in industrial conditions their content in the oil was 8.7 mg/g. In extracted oil the level of phytosterols was 25% higher then in pressed oil. During refined process, especially neutralization, the decrease of phytosterols content was 20%. Using the rapeseed oil to multiple deep frying of French fries generated next 64% losses. The most important reasons for phytosterols content decrease are their autoxidation processes. Autoxidation of sterols is theorized to be a free radical process. Some processes, such as refining plant oils and deep frying or storage, intentionally induce oxidation to produce phytosterol oxidation products (oxyphytosterols). They were determined in rapeseeds on the level 10-15 μg/g of seeds. During industrial production of rapeseed oil the content of oxyphytosterols systematically increased and after refining it was 100-110 μg/g. During multiple deep-frying of French fries in rapeseed oil oxyphytosterols content increased and after 14th frying their content was 200 μg/g in used oil and 150 μg/g in French fries. Oxyphytosterols are absorbed by humans and their subsequent metabolic conversions may be of toxicological significance.
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Podjęto próbę scharakteryzowania makrostruktury cząstek proszku i struktury wewnętrznej mikrokapsułek estrów etylowych oleju roślinnego otrzymywanych metodą suszenia rozpryskowego w warunkach laboratoryjnych i przemysłowych. Z wykorzystaniem techniki komputerowej analizowano mikrofotografie proszków otrzymane za pomocą elektronowego mikroskopu skaningowego. Stwierdzono duży zakres zmian wielkości cząstek, w przedziale 3,9-124,8 μm, z przewagą cząstek poniżej 40 μm. Elementy struktury wewnętrznej miały średnice w zakresie 0,141-1,915 μm, odpowiadające średnicom kropel fazy olejowej w emulsjach przed suszeniem. W obu przypadkach rozkład wielkości mierzonych elementów obrazu był logarytmiczno-normalny. Wykazano, że różne warunki preparowania emulsji i suszenia rozpryskowego prowadzą do istotnych statystycznie różnic parametrów struktury cząstek - zarówno zewnętrznej, jak i wewnętrznej. Stwierdzono konieczność korelowania parametrów charakteryzujących strukturę mikrokapsułek ze strukturą emulsji i innymi właściwościami proszków.
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