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In presented research a method for remediation of MCPA contaminated soil based on the combination of fluidization process technique and ozone was utilized. Exposition of soil contaminated with MCPA at the concentration of 0,1 % w/w on the a mixture of air and ozone allowed for removal of over 90% of MCPA in form of MCPA 2-ethylhexanol-ester (MCPA 2-EHE) in laboratory conditions. The efficacy of the method was proven by GC-MS analysis of MCPA concentration profile during soil remediation procedure and by comparing of kinetic data, such as half life time, with the natural half life time of MCPA in the soil.
Some basic experiments of nitrous oxide determination, utilizing a classic double beam spectrometer were performed. Known amounts of nitrous oxide were measured in atmospheric air, helium and exhaust gases. The experiments allowed determination of N20 in amounts of 80 and 4 ppmv, employing standard and long-path gas cells, respectively.
Stilbene synthases (STSs) are enzymes that play a critical role in the biosynthesis of stilbene, phytoalexins in a small number of unrelated plant species, and are induced by various biotic and abiotic stressors like pathogen attack, UV-irradiation or ozone exposure. To investigate the molecular basis for ozone-induced plant stress responses, we have examined the promoter of the grapevine resveratrol synthase (Vst1). In this report we summarize the influence of ozone on gene regulation. In transgenic tobacco a chimeric gene construct, containing the Vst1 promoter combined with the β-glucuronidase (GUS) reporter gene, is rapidly induced by ozone (0.1 µl·l⁻¹, 12 h). The same construct is also strongly induced by ethylene (20 µl·l⁻¹, 12 h). Promoter deletion analysis of the 5′ flanking sequence identified a positive regulatory element between −430 bp and −280 bp. This region contains ethylene-responsive enhancer elements, as well as an elicitor-responsive sequence in inverse orientation.
Residues of plant protection products can be nowadays found in almost all samples of fruits as even if their application was carried out with respect to standards of Good Agricultural Practice. The intake of these compounds with food of plant origin may result in various disorders and diseases. Since the use of plant protection agents seems unavoidable, it is necessary to treat contaminated food material to eliminate or reduce residues content within food products. Ozone is utilized for elimination of biological and chemical contaminants in various environmental matrixes. Since its utilization in food industry has been permitted many experiments were conducted in order to determine its efficiency and side effects on food quality. The goal of this paper is to contribute to a discussion on the ozonization as a process leading to reduction of pesticide residues on plant surface.
The geographic position, astronomic factors (e.g. the Earth’s maximum distance from the Sun during winter), ice cover and altitude are the main factors affecting the climate of the Antarctic, which is the coldest place on Earth. Parts of Antarctica are facing the most rapid rates of anthropogenic climate change currently seen on the planet. Climate changes are occurring throughout Antarctica, affecting three major groups of environmental variables of considerable biological signifi cance: temperature, water, UV-B radiation. Low diversity ecosystems are expected to be more vulnerable to global changes than high diversity ecosystems.
Opracowano metodę sporządzania trwałych wzorców barwnych do kolorymetrycznego oznaczania śladowych ilości ozonu w warunkach polowych.
Ozone-sensitive and -resistant tobacco plants and an ozone-sensitive bean were employed in this experiment. Plants were exposed at two different sites varying in ozone level, within Poznań city and in a remote forestry area. Net photosynthetic rate (PN), stomatal conductance (gs ) and intercellular CO₂ concentration (Ci ) were measured every 7th day until the 28th day of the experiment. The ozone concentrations were higher at the forest site (called here the high ozone site) than in the city (low ozone site). Mean hour ozone concentrations at forest site varied between 34.2–45.5 ppb and 30.2–39.2 ppb, while cumulative ozone concentrations (AOT 40) were 2032 ppb h⁻¹ and 611, respectively. The aim of the study was to compare the results of exposed ozone-sensitive and resistant test plants according to the potential effect of tropospheric ozone on natural vegetation. The experiment revealed the variability between exposure sites and plant species, as well as changes of photosynthetic parameters during the whole experimental season. Common bean assimilation parameters revealed the best correlation with the tropospheric ozone level. Tobacco test plants – ozone-sensitive and -resistant – varied in response to stress factors. There were no statistical differences between exposure sites and plant response. However, both ozone-sensitive and -resistant tobacco plants revealed lower levels of PN at the low ozone site, while ozone-sensitive bean revealed the opposite relation. Moreover, plants revealed gas exchange relations which were not previously observed in fumigation experiments (such as small differences in Ci levels of tobacco plant in the first three weeks). This might be a result of relatively low levels of tropospheric ozone during the experimental period. Hence, it is suggested to continue ambient air investigations to confirm or reject the results of this experiment.
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