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Background. The aim of the study was to investigate the effects of a diet modification and supplementation with B-group vitamins, on selected characteristics of calcium and magnesium management in rats. Material and methods. The experiment was carried out on 60 rats aged 5 months. Animals were fed two different diets. Groups I and II received clean tap water to drink, while group III had water solution of group-B vitamins. Results. Analysis of blood plasma calcium and magnesium concentrations in the studied animals did not reveal a significant effect of the analysed factors on blood plasma calcium concentration in examined rats. An increase of the plasma level of magnesium was observed with a change in the diet composition. The supplementation reduced magnesium level to those observed in animals fed a basic feed. Diet modification and supplementation exerted the influence on whole blood calcium and magnesium levels. A change in the composition of the diet and its supplementation results also in an increase in bone calcium content in males, and in an increase in bone magnesium content in females. Conclusions. Lack of changes in blood plasma calcium levels in the studied animals implies the preservation of the homeostatic mechanisms that regulate its concentration, whereas the observed significant changes in the concentration of magnesium, point to a significant effect of this factor on its metabolism. Changes in hematocrit indicator, whole blood concentrations of calcium and magnesium and the absence of changes in concentrations of these elements in blood plasma of supplemented animals may indicate that the elements move to erythrocytes, which may imply a distortion of cellular membrane and an increase in its permeability. Composition of the diet and its supplementation modified also bone calcium and magnesium concentrations in the studied rats.
Badano wpływ doustnego 8 tyg. narażenia szczurów na kadm 5 i 50 mg/dm3, wprowadzonego do ustroju w postaci wodnych roztworów CdCl2, na wybrane wskaźniki gospodarki wapniowej. Stwierdzono wzrost wydalania wapnia z moczem oraz obniżenie jego stężenia w surowicy, kości udowej i nerce. Poziom tego pierwiastka w wątrobie i śledzionie nie uległ zmianie.
The aim of this experiment was to evaluate the effect of "anionic salts", such as ammonium chloride (NH₄Cl), aluminium sulphate (Al₂(SO₄)₃·16 H₂O), ammonium sulphate (NH₄)₂SO₄, and calcium chloride (CaCl₂·2H₂O), on the calcium metabolism, copper, zinc, and molybdenum concentrations and superoxide dismutase (SOD) activity in bovine blood. The experiment was carried out on 26 cows over a 2 year period. Cattle were fed daily fodder containing the chemical compounds. During the first year, two districts (A - experimental, B - control) were evaluated for deficiency diseases. During the second year, blood concentrations of calcium, phosphorus, magnesium, copper, zinc, molybdenum, and alkaline phosphatase (ALP) and SOD activity were evaluated. Urine pH was also determined. Before and after supplementation, the dietary cation anion difference (DCAD) was evaluated and a mineral fodder ration was formulated for the cows. There were significant differences in the concentration of chlorine and sulfur in the fodder of cows between group A and B after supplementation. Mineral supplementation with chlorides and sulphates is particularly advantageous for systemic acidification. It consisted of the occurrence of lower pH and higher blood concentration of calcium and copper and higher activity of SOD in the urine of experimental cows. This can be seen as improvement of calcium metabolism indicators. DCAD balance was reduced from +241.5 mmol/kg DM to -79.5 mmol/kg DM diet. An increase in ALP activity to normal levels was seen when the animals were given chlorides and sulphates. The study showed that "anionic salts" had a positive effect on mineral metabolism and antioxidant status of dairy cows.
Badano wpływ wielokrotnego narażenia na tiuram (4 tygodnie) w dawce 5% LD50, na transport wapnia i fosforu w jelicie oraz stężenie tych pierwiastków w surowicy i kości udowej szczurów karmionych dietami o różnej zawartości białka (7,5%, 10,3%, 22,7% - dieta standardowa i 30,6%).
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