Results of crosses between Hordeum bulbosum (2x) and H. vulgare (2x) are presented. Nine H. vulgare genotypes of different crossability with H. bulbosum were treated both as female and male parents. Immature embryos were cultured in vitro according to ADAMSKI and SURMA (1994). Seeds set, embryo development and obtained haploid plants were recorded. It was shown that seed setting and haploid plant efficiency (haploids/100 florets) were about ten times lower in H. bulbosum x H. vulgare than in H. vulgare × H. bulbosum The cvs. Vada, Apex and the doubled haploid line KA7/3 (related to Apex) which showed a low crossability in H. vulgare × H. bulbosum, gave a relatively high seed setting in reciprocal crosses. The obtained results indicate that partial incompatibility of some H. vulgare genotypes with H. bulbosum could be cytoplasmatically influenced.