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1

Uwagi o metodach oznaczania liczby nadtlenkowej

100%
Bronisz H., Raciborska I.
Roczniki Państwowego Zakładu Higieny
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1954
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tom 05
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nr 1
2

Uwagi w związku z jełczeniem tłuszczów ze specjalnym uwzględnieniem boczków

100%
Bronisz H., Raciborska I.
Roczniki Państwowego Zakładu Higieny
|
1954
|
tom 05
|
nr 2
3

Orientation tuning in mouse primary visual cortex – Intrinsic signal optical imaging study

81%
Raciborska I., Kordecka K., Posluszny A., Waleszczyk W. J.
Acta Neurobiologiae Experimentalis
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2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Accumulating body of research has shown a cardinal bias for preference of spatially oriented targets in different species including humans, indicating greater neuronal responses in the primary visual cortex for horizontal or vertical contours in opposite to oblique ones. AIM(S): We used intrinsic signal optical imaging, a popular tool to map cortical function in rodents to verify the hypothesis whether a cardinal bias is present also in mouse primary visual cortex. METHOD(S): The experiments were performed on 7 week old wild mice under isoflurane anaesthesia. Intrinsic signals were recorded using CCD camera set above the visual cortex. Visual stimuli, square-wave black-and-white gratings (spatial frequency 0.05 cycle/degree, and temporal frequency 2 Hz, four orientations: 0, 45, 90, 135 degree) drifting in two directions, back and forth, were presented in random order with uniform grey images in 16 trials. Imaging was performed under the control of Imager 3001 system. Data were collected with 10 Hz resolution from 1 s before stimulus onset, during 7 s of visual stimulation and to 1 s after stimulus offset with 7 s interval between recordings. RESULTS: Using the described protocol of visual stimulation and data collection we could successfully map cortical responses to visual stimuli of different orientations. Collected images showed the strongest responses for horizontally and vertically oriented gratings. CONCLUSIONS: Our results support the hypothesis of the bias toward cardinal orientation preference in mouse visual cortex. FINANCIAL SUPPORT: Supported by the Polish National Science Center grant Symfonia 1 (2013/08/W/ NZ4/00691).
4

The influence of associative pairing of visual stimulation and tail shock on somatostatin expression in mouse primary visual cortex

81%
Raciborska I., Popiolkiewicz J., Waleszczyk W. J.
Acta Neurobiologiae Experimentalis
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2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Previous study showed that associative pairing involving monocular visual stimulation resulted in a significant increase of the density of cells expressing glutamic acid decarboxylase (GAD), γ-aminobutyric acid (GABA) synthesizing enzyme, in mouse primary visual cortex contralateral to the stimulated eye. The effect was attributed to the group of parvalbumin- (PV)-negative interneurons, since the density of PV-positive cells remained unaffected. The aim of the present study is to identify the group(s) of PV-negative GABA-ergic cells in mouse visual cortex that are mobilized by visual training combined with tail shock. METHODS: The same method of associative pairing (classical conditioning) involving monocular visual stimulation was used. We used nine young adult male mice, which were divided into two experimental groups: trained (5 mice) and naïve (4 mice) that served as a control. During three days lasting training, monocularly presented visual stimulus, drifting gratings of optimal spatial and temporal frequency (conditioned stimulus, CS), was coupled with electric shock applied to the tail (unconditioned stimulus, UCS) at the end of visual stimulation. Twenty four hours after the training (CS+UCS), mouse brains were subject to immunohistochemistry. The brain slices were photographed through fluorescent microscope and analyzed quantitatively to determine the amount of fluorescence using Fiji ImageJ software. RESULTS: Monocular visual training combined with tail shock resulted in a significant increase of GAD- and SOM-positive puncta in the contralateral primary visual cortex of (CS+UCS) mice in comparison to naive group. CONCLUSIONS: Our results confirm earlier finding of the upregulation of cortical GABA-ergic system by classical conditioning involving sensory stimulation and indicate on engagement of SOMpositive interneurons.
5

Mouse monocular visual classical conditioning reduces spontaneous firing of excitatory layer 4 cortical neurons – ex vivo studies on brain slices

71%
Bekisz M., Shendye N., Raciborska I., Wrobel A., Waleszczyk W. J.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: It is accepted that learning induces plastic changes in adult neocortex. Our previous experiments on mice showed that classical conditioning in which monocular visual stimulation was paired with an electric shock to the tail enhanced GABA immunoreactivity within layer 4 of the monocular part of the primary visual cortex (V1), contralateral to the stimulated eye. AIM(S): In the present study we investigated whether the same classical conditioning paradigm induced changes of neuronal excitability in this cortical area. METHOD(S): We performed patch-clamp whole-cell recordings from ex vivo slices of mouse V1. Two experimental groups were used: mice that had 7-day visual classical conditioning and control animals. The slices were perfused with the modified artificial cerebrospinal fluid, the composition of which better mimics the brain interstitial fluid in situ and induces spontaneous activity in slices. The frequency of spontaneous action potentials was calculated as a general measure of neuronal excitability. RESULTS: We found that layer 4 excitatory cells located in the monocular representation of the “trained” eye in V1 had lower frequency of spontaneous action potentials than neurons from the same cortical region of control animals. CONCLUSIONS: Weaker spontaneous firing indicates decreased general neuronal excitability within layer 4 of the monocular representation of the “trained” eye in V1. Such effect could result from enhanced inhibitory processes in this cortical area. FINANCIAL SUPPORT: Supported by the Polish National Science Center grant Symfonia 1 (2013/08/W/NZ4/00691) and the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no 665735 (Bio4Med) and by the funding from Polish Ministry of Science and Higher Education within 2016–2020 funds for the implementation of international projects (agreement no 3548/H2020/COFUND/2016/2).
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