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Rapid epithelial restitution is an important protective mechanism which enables the gastrointestinal mucosa to reestablish epithelial integrity following superficial injury within hours. In this study we examined the influence of an acidic luminal pH, removal of the necrotic layer, nutrient bicarbonate, calcium and sodium desoxycholate (Na-DOC) on restitution in the rabbit duodenum in vitro and the role of Na-DOC and calcium for rapid restitution of the human colon in vitro. Transmucosal potential difference (PD), short-circuit current (lsc) were measured and resistance against passive ion flux (R) was calculated. Electrophysiological changes paralleled morphological injury but did not necessarily reflect restitution in all experiments. The extent of mucosal injury was assessed by computerized real-time morphometry. 5 hrs after luminal exposure to 10 mH HCl for 10 min residual damage (RD) was 14% in the duodenum. Luminal pH of 3.0 (RD of 30%), removal of necrotic layer at acidic luminal pH (RD of 66%), absence of bicarbonate from the serosal solution (RD of 35 % at neutral luminal pH; RD of 96% at acidic luminal pH) and removal of calcium from the serosal solution (RD of 58 %) impaired restitution in the duodenum. Continuous postinjury luminal Na-DOC exposure did not influence restitution in the duodenum (RD of 19%). 5 hrs after luminal exposure to 0.5 mM Na-DOC for 10 min RD was 26% in the human colon. Continuous postinjury luminal Na-DOC exposure (RD of 51 %) and removal of calcium from the nutrient solution (RD of 65 %) impaired restitution in the human colon. Thus we conclude that restitution of the rabbit duodenum in vitro requires a necrotic layer and bicarbonate flux to withstand acidic luminal pH, while restitution is not Effected by Na-DOC. In the human colon Na-DOC inhibits restitution. Both the duodenum and colon require calcium for rapid restitution.
Data from floristic publications, forest management records, comprehensive questionnaires and original field research were used to assess the silver fir resources in the Sudety Mts. Fir trees older than 50 years were recorded in over 2000 localities but the mean number of individuals per locality was only about 15. The generally low number of fir trees and their considerable dispersion are the major causes of the poor reproduction of the studied species in the Sudety Mts. In that region, silver fir should receive special treatment and, if possible, excluded from logging plans. Current and projected activities concerned with fir protection are described briefly.
In the 40 fir localities in the Sudety Mountains, varying in site conditions and the degree of natural regeneration development were taken soil samples from the mineral horizon and were subjected to particle size distribution analysis. The best regeneration of fir stands has been reported on medium-textured soils, containing a few percents of the clay fraction and from 50to 60% of sand.The poorest regeneration was reported on silty soils, located in the lower parts of slopes. In comparision with the Carpathians, spectrum of conditions in which firs grow in the Sudety Mountains is clearly shifted from coarse-textured soils to medium-textured soils.
Background. The majority of European populations of vimba, Vimba vimba (L.), have been classified into increasing categories of conservation status while Polish populations of this fish are classified as CR (critically endangered) and in fact they are facing extinction. In many rivers in Poland, including the Odra River, restitution efforts of vimba have been undertaken. Monitoring some biological parameters of the population such as the rate of growth (increase in mass and length), age structure, sex structure, and condition nay help to evaluate the success rate of the restitution efforts. Materials and methods. In total, 210 individuals of vimba, caught in the waters of the southern part of the Odra River estuary, mainly in Lake Dąbie, between 28 May 2007 and 30 June 2008 were studied as to their age, condition, rate of growth in mass and length. Making use of the linear R–L dependence, the back calculations were made according to the Rosa Lee procedure with a standard of 20 mm. Results were approximated by 5 mathematical models of growth proposed by von Bertalanffy, Ford–Walford, Gompertz, and based on second degree polynomial function and modified power function. Increase in mass was described by a modified von Bertalanffy equation. The condition of the fish was described by the Fulton (K) and Le Cren (Lc) formulae. Results. Females dominated in the material studied, they constituted over 61% of all individuals. In the age structure groups, females were most often found in groups: 10+ (13.33%), 9+, and 11+ (12.86% each). Males were found in the greatest number among 5-, 6-, and 7-year-old fish (4+, 5+, and 6+, respectively). The mean standard length and mass of the vimba fish studied were 306.33 mm and 286.02 g, respectively. The annual increase in length of the fish approximated 20 mm, being the most intense in the first two years of life (23–24 mm). Fulton and Le Cren coefficients of condition were 0.93 and 1.64 and the ranges of these values were 0.69–1.47 and 1.15–2.60, respectively. Conclusion. Analysis of back readings and empirical results proved that the growth of vimba was best approximated by the von Bertalanffy model. Comparative analysis of condition coefficients of the population of vimba in the Odra River estuary and other populations showed that the condition of analysed vimba population in individual years of live was lower.
W celu zwiększenia skuteczności ochrony ex situ rodzimej różnorodności biologicznej (wynikającej z „Krajowej strategii ochrony i zrównoważonego użytkowania różnorodności biologicznej wraz z Programem Działań na lata 2007-2013”), w Ogrodzie Botanicznym w Bydgoszczy IHAR w 2008 roku przystąpiono do odtwarzania naturalnych zbiorowisk roślinnych. Wykonano stanowiska dla halofitów oraz roślinności wydmowej. Prace zostały poprzedzone obserwacjami fitosocjologicznymi oraz badaniami glebowymi wybranych stanowisk in situ: Rogowo i Włodarka k. Trzebiatowa (wydma nadmorska oraz łąka halofitowa), okolice Solca Kujawskiego (wydma śródlądowa) oraz Janikowo k. Inowrocławia (solnisko). Na odtworzonych stanowiskach wysadzono rośliny uzyskane z nasion zebranych w warunkach naturalnych. Największe zadarnienie odnotowano na solnisku z podłożem odpadowym z Zakładów Sodowych w Janikowie. Gatunki wydmowe wytworzyły mocne systemy korzeniowe, przyczyniając się do ustabilizowania stanowiska.
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