Wyniki wyszukiwania

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Psychrotrophic lipase producers from Arctic soil and sediment samples

100%

Rasol R., Rashidah A. R., Nur Nazuha R. S., Smykla J., Wan Maznah W. O., Alias S. A.

Polish Journal of Microbiology

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2014

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tom 63

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nr 1

Culturable microorganisms were successfully isolated from soil and sediment samples collected in 2011 on the northern coast of Hornsund, West Spitsbergen. A total of 63 single colony isolates from three sampling sites obtained were subjected to temperature dependence study to assess whether they are obligate psychrophilic or psychrotrophic strains. From initial temperature screening, only 53 psychrotrophic isolates were selected that are capable of growing between 4–28°C. The rest that were capable of tolerating higher temperatures up to 37°C were not included in this study. These isolates were chosen for lipase enzyme screening confirmation with the standard plate assay of olive oil and fluorescent dye Rhodamine B. Six lipase positive isolates were also subjected for subsequent lipase enzyme plate screening on tributyrin, triolein, olive oil and palm oil agar. Lipase production by these six isolates was further assayed by using colorimetric method with palm oil and olive oil as the substrate. These isolates with promising lipase activity ranging from 20 U/ml up to 160 U/ml on palm oil and olive oil substrate were successfully identified. Molecular identification by using 16S rRNA revealed that five out of six isolates were Gram-negative Proteobacteria and the other one was a Gram-positive Actinobacteria.

2

Comparison of the microbiological qualities of turkey myofibrillar protein isolate, mechanically recovered turkey meat and turkey breast

67%

Malicki A., Bruzewicz S., Zechalko-Czajkowska A., Szybiga K., Kopec W., Trziszka T.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2006

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tom 09

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nr 4

3

Zmiany lipolityczne w mleku UHT

59%

Panfil-Kuncewicz H., Zuchowska M., Kuncewicz A.

Acta Academiae Agriculturae ac Technicae Olstenensis. Technologia Alimentorum

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1998

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tom 30

91-100

4

Identification of the csp gene and molecular modelling of the CspA-like protein from Antarctic soil-dwelling psychrotrophic bacterium Psychrobacter sp. B6

59%

Kaufman-Szymczyk A., Wojtasik A., Parniewski P., Bialkowska A., Tkaczuk K., Turkiewicz M.

Acta Biochimica Polonica

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2009

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tom 56

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nr 1

63-69

We cloned and sequenced the cspA-like gene from a psychrotrophic Antarctic soil-dwelling bacterial strain Psychrobacter sp. B6. The gene is 213 bp long and shows 99% and 98% sequence identity with the Psychrobacter cryohalolentis K5 gene encoding a cold-shock DNA-binding domain protein and the Psychrobacter arcticus transcriptional regulator-CspA gene, respectively. The protein encoded by the Psychrobacter sp. B6 cspA-like gene shows 100% identity with the two proteins mentioned above, and also 61% sequence identity with CspB from Bacillus subtilis and Csp from Bacillus caldolyticus, and 56% — with Escherichia coli CspA protein. A three-dimensional model of the CspA-like protein from Psychrobacter sp. B6 was generated based on three known structures of cold shock proteins: the crystal structure of the major cold shock protein from Escherichia coli (CspA), the NMR structure of the latter protein, and the NMR structure of Csp from Thermotoga maritima. The deduced structure of the CspA-like protein from Psychrobacter sp. B6 was found to be very similar to these known structures of Csp-like proteins.

5

Modelowanie przezywalnosci psychrotrofow w twarogach pakowanych hermetycznie

59%

Steinka I.

Bromatologia i Chemia Toksykologiczna

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2008

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tom 41

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nr 3

585-589

6

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Jakosc mikrobiologiczna mleka krowiego znajdujacego sie poza oficjalnym obrotem

43%

Grega T., Sady M., Siemek S.

Żywność Technologia Jakość

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1997

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tom 04

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