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High content of MyHC II in vastus lateralis is accompanied by higher VO2/power output ratio during moderate intensity cycling performed both at low and at high pedalling rates

100%

Majerczak J., Szkutnik Z., Karasinski J., Duda K., Kolodziejski L., Zoladz J. A.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 2

The aim of this study was to examine the relationship between the content of various types of myosin heavy chain isoforms (MyHC) in the vastus lateralis muscle and pulmonary oxygen uptake during moderate power output incremental exercise, performed at low and at high pedalling rates. Twenty one male subjects (mean ± SD) aged 24.1 ± 2.8 years; body mass 72.9 ± 7.2 kg; height 179.1 ± 4.8 cm; BMI 22.69 ± 1.89 kg . m-2; VO2max 50.6 ± 5.3 ml . kg . min-1, participated in this study. On separate days, they performed two incremental exercise tests at 60 rev . min-1 and at 120 rev . min-1, until exhaustion. Gas exchange variables were measured continuously breath by breath. Blood samples were taken for measurements of plasma lactate concentration prior to the exercise test and at the end of each step of the incremental exercise. Muscle biopsies were taken from the vastus lateralis muscle, using Bergström needle, and they were analysed for the content of MyHC I and MyHC II using SDS - PAGE and two groups (n=7, each) were selected: group H with the highest content of MyHC II (60.7 % ± 10.5 %) and group L with the lowest content of MyHC II (27.6 % ± 6.1 %). We have found that during incremental exercise at the power output between 30 - 120 W, performed at 60 rev . min-1, oxygen uptake in the group H was significantly greater than in the group L (ANCOVA, p=0.003, upward shift of the intercept in VO2 / power output relationship). During cycling at the same power output but at 120 rev . min-1, the oxygen uptake was also higher in the group H, when compared to the group L (i.e. upward shift of the intercept in VO2 / power output relationship, ANCOVA, p=0.002). Moreover, the increase in pedalling rate from 60 to 120 rev . min-1 was accompanied by a significantly higher increase of oxygen cost of cycling and by a significantly higher plasma lactate concentration in subjects from group H. We concluded that the muscle mechanical efficiency, expressed by the VO2 / PO ratio, during cycling in the range of power outputs 30 - 120 W, performed at 60 as well as 120 rev . min-1, is significantly lower in the individuals with the highest content of MyHC II, when compared to the individuals with the lowest content of MyHC II in the vastus lateralis.

2

Growth and antioxidant activity of Desulfotomaculum acetoxidans DSM 771 cultivated in acetate or lactate containing media

100%

Pawlowska-Cwiek L., Pado R.

Polish Journal of Microbiology

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2007

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tom 56

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nr 3

Three independent 28 or 32-day stationary cultures of Desulfotomaculum acetoxidans DSM 771 strain were carried out under anoxic conditions in acetate or lactate-containing media. The acids were the sole carbon and energy sources in these media. During cultivation the turbidity (for calculation of cell division index) and hydrogen sulfide contents were determined in culture broth and reduced glutathione and protein concentrations were assayed in culture broth supernatant. In these three successive cultures, the bacterium initially grew much faster on lactate than on acetate. However, after two weeks of culture this difference disappeared and in fact the growth rate was higher on acetate than on lactate. The level of H₂S formed (product of the dissimilatory pathway of sulfate reduction) demonstrated that this pathway was more effective when lactate was a carbon source and the average H₂S concentration was from over 3-fold to about 9-fold greater in lactate than in acetate cultures. Also GSH (glutathione, product of the assimilatory sulfate reduction pathway) average level was about 2-fold higher in lactate-grown cultures. The high negative values of the correlation coefficients between GSH and O, levels, especially during the first 4 days of cultivation, indicate that GSH is a very important antioxidizing extracellular agent of D. acetoxidans. The rapid increase in GSH level, preceding the release of H₂S, indicates the metabolic priority of the assimilation pathway of sulfate reduction. For both carbon sources the highest coefficient of correlation was found between protein and H₂S levels. These results suggest that hydrogen sulfide is bound by proteins (which contain cysteinyl residues) secreted by D. acetoxidans cells. Indicated way of H,S bounding could result in its acccumulation. This coefficient of correlation increased gradually in the successive cultures. The ratio of H₂S concentration to protein concentration increased gradually in the successive cultures, too.

3

Step and ramp induction of myocardial ischemia: comparison of in vivo and in silico results

100%

Salem J. E., Cabrera M. E., Chandler M. P., McElfresh T. A., Huang H., Sterk J. P., Stanley W. C.

Journal of Physiology and Pharmacology

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2004

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tom 55

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nr 3

This study tested the robustness of our computational model of myocardial metabolism by comparing responses to two different inputs with experimental data obtained in pigs under similar conditions. Accordingly, an abrupt and a gradual reduction in coronary flow of similar magnitude were implemented and used as model input. After flow reductions reached 60% from control values, ischemia was kept constant for 60 min in both groups. Our hypotheses were that: (1) these two flow-reduction profiles would result in different transients (concentrations and flux rates) while having similar steady-state values and (2) our model-simulated responses would predict the experimental results in an anesthetized swine model of myocardial ischemia. The two different ischemia-induction patterns resulted in the same decrease in steady-state MVO2 and in similar steady-state values for metabolite concentrations and flux rates at 60 min of ischemia. While both the simulated and experimental results showed decreased glycogen concentration, accumulation of lactate, and net lactate release with ischemia, the onset of glycogen depletion and the switch to lactate efflux were more rapid in the experiments than in the simulations. This study demonstrates the utility of computer models for predicting experimental outcomes in studies of metabolic regulation under physiological and pathological conditions.

4

Evaluation of glycolytic potential usefulness for diagnosis of meat quality in pigs free of RYR1T and RN- alleles

84%

Przybylski W., Kocwin-Podsiadla M., Krzecio E.

Annals of Animal Science. Supplement

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2005

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nr 2

5

Changes in lactate, pyruvate and glucose levels in field hockey players under different training and match stimuli

84%

Podgorski T., Adrian J., Nowak A.

Trends in Sport Sciences

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2015

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tom 22

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nr 3

Introduction. Lactate, pyruvate and glucose levels are the most common biochemical markers used for controlling training loads and physical efficiency of athletes. Each stage of a field hockey training cycle requires activation of a different metabolite responsible for physical exercise. Aim of Study. The aim of the study was to determine the biochemical response of field hockey players to different types of exercise in a training cycle in comparison to a real match. Material and Methods. Ten male university team field hockey players took part in the study. The players were examined six times during an annual training cycle. The examination consisted of the following tests: treadmill test (twice), 13 km running, interval training, spinning, and a real field hockey match. During the tests preand post-exercise capillary blood from a fingertip was collected to determine the lactate (La), pyruvate (Pa) and glucose levels using enzyme methods. Furthermore, bioelectrical impedance analysis (BIA) was carried out four times during the annual training cycle. Results. Each exercise test increased the La and Pa concentrations, however, the glucose level was raised only during the treadmill tests. The 13 km running test and interval training results were statistically different. The most essential changes of La and Pa concentrations were noted between the treadmill tests and the match. Conclusions. The aim of field hockey training is to prepare players to meet match requirements. The analysis of players’ metabolic responses to different kinds of training indicates that a match effort is similar to 13 km running and physiologically close to interval training

6

Lactate in cardiogenic shock - current understanding and clinical implications

84%

Kubiak G. M., Tomasik A. R., Bartus K., Olszanecki R., Ceranowicz P.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 1

7

Early effects of short-term endurance training on hormonal responses to graded exercise

84%

Chwalbinska-Moneta J., Kruk B., Nazar K., Krzeminski K., Kaciuba-Uscilko H., Ziemba A.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 1

Twelve male, sedentary volunteers (22.0 ± 0.7 yrs) were submitted to three weeks of a bicycle ergometer training, consisting of 45 min exercise (at 70% VO2max), 4 times in the first week and 3 times in the next 2 weeks. They performed four incremental exercise tests with the power output increased by 50 W every 3 min until volitional exhaustion: two before training (C1 and C2), and after one (T1) and three (T3) weeks of training. Before and after each load the plasma noradrenaline (NA), adrenaline (A) and blood lactate (LA) concentrations were determined in venous blood samples as well as plasma growth hormone (HGH) and cortisol concentrations before and at the end of exercise. A decrease in NA concentration was found already after 1 week of training at power output of 100 W (p<0.01) and 200 W (p<0.05). Similar decline was maintained after 3 weeks of training. No significant training-induced differences in plasma A concentration were found, however, the thresholds for both catecholamines were significantly shifted towards higher values after 3 weeks of training. One week of training caused a decrease in the pre-exercise (p<0.01), as well as post-exercise (p<0.05) plasma cortisol and HGH concentrations. It was concluded that endurance training induced a decrease in HGH, cortisol and NA concentration already after one week of training. A decline of pre-exercise plasma HGH and cortisol levels with time of experiment may, in part, indicate familiarization to exercise protocol.

8

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Muscle biochemistry in rainbow trout Oncorhynchus mykiss following Yersinia ruckeri vaccination

67%

Tkachenko H., Grudniewska J., Pekala A.

Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

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2016

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tom 20

Vaccination of rainbow trout against Yersiniosis confers a high degree of protection to the fish (Raida et al. 2011). On the other hand, vaccination could alter metabolitic reactions in organisms. Therefore, exploring the effects of vaccination against Y. ruckeri on health condition of trout in general, and oxidative stress biomarkers and biochemical alterations in different tissues specifically, would be valuable. This prompted us to investigate theeffects of vaccination against Y. ruckeri on muscle function, and the oxidative mechanism underlying those effects, by detecting relevant lipid peroxidation (2-thiobarbituric acid reactive substances, TBARS) and protein oxidation biomarkers (aldehydic derivatives and ketonic derivatives) as well as biochemical alterations (aminotransferases and lactate dehydrogenase activity, lactate and pyruvate levels) in rainbow trout Oncorhynchus mykiss following Y. ruckeri vaccination at first month after oral immunization. Concentrated vaccine with inactivated by formalin Y. ruckeri strains was enclosed by fish feed, and was administered three times every other day. Rainbow trout from each group were euthanized 30 days after the immunization, and then muscle tissue were sampled for analysis. The TBARS level in the muscle tissue of vaccinated group was at same level compared to unhandled group. The ketonic derivatives of oxidatively modified proteins in the trout following Y. ruckeri vaccination at first month after immunization were significantly increased compared to the level in the controls, while the aldehydic derivatives of oxidatively modified proteins were non-significantly increased. Pyruvate level was increased by 47% (p = 0.013) in vaccinated trout compared to values of untreated fish. Lactate level, aminotransferases and lactate dehydrogenase activities were nonsignificantly altered in vaccinated trout. Our results suggest that vaccination could promote the activation of the gluconeogenic substrate-providing enzymes, as well as substrates for aerobic metabolism that might in turn contribute to increase of oxidatively modified proteins. The oxidative stress biomarkers, i.e. content of oxidative protein damage, as well as biochemical enzymes and substrates were sensitive to vaccination of trout against Y. ruckeri and may potentially be used as biomarkers in evaluating vaccine toxicity in rainbow trout. From a practical point of view, the results may be useful in relation to studies of infections and the development, administration and uptake of new vaccines applicable for large amounts of fish.

9

Exercise-induced biochemical alterations in the blood of horses involved in recreational horseback riding in Pomeranian Region (Northern Poland)

67%

Tkachenko H., Witaszek M., Pazontko-Lipinski P.

Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

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2018

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tom 22

The aim of our study was to determine changes in some biochemical indices (alanine (ALT) and aspartate aminotransferase (AST), lactate dehydrogenase (LDH)) as well as lactate and pyruvate level in the blood of horses exercised in recreational horseback riding from Pomeranian regions during a training session. Measurement of values of liver biomarkers (AST, ALT) and muscle damage indicator (LDH), followed by a variety of training programs, can help to better understand the acute and chronic effects of resistance training. Thirteen healthy adult horses from central Pomeranian region in Poland (village Strzelinko, N54°30'48.0" E16°57'44.9"), aged 9.5±2.4 years old, were used in this study. All horses participated in recreational horseback riding. Training started at 10.00 AM, lasted 1 hour and consisted from a ride of cross country by walking (5 min), trotting (15 min), walking (10 min), trotting (10 min), walking (5 min), galloping (5 min), and walking (10 min). Blood was drawn from jugular veins of the animals in the morning, 90 minutes after feeding, while the horses were in the stables (between 8.30 and 10.00 AM), and immediately after exercise session (between 11.00 AM and 2.00 PM). Blood was stored in tubes with K3-EDTA and sodium citrate (3.8%) and held on ice until centrifugation at 3,000 rpm for 15 minutes. The plasma was removed. Plasma was used for the determination of aminotransferases and lactate dehydrogenase activity; whole blood was used for determination of lactate and pyruvate level. The regular training lead to adaptive processes which provoke changes in biochemical indices. In our research, non-significant alterations of AST and LDH activities in horses involved in recreational horseback riding were observed. This may indicate a normal course of aerobic-anaerobic glycolysis in horses involved in recreational horseback riding during a training session. Moreover, ALT activity was decreased by 20.6% (p = 0.000) during a training session. Increased blood lactate level in horses involved in recreational horseback riding during training session could be explained by increasing lactate formation via the reduction of pyruvate catalyzed by LDH as a result of anaerobic energy supply. Based on these results, it is concluded that the endurance exercises lead to specific metabolic changes accompanied by a redistribution of energy supply for improving resistance to exercises and athletic performance of horses. Therefore, the present data can be useful to assess the status of athletes and the degree of their training adaptability providing an opportunity to modify the training schedule to achieve the desired performance.

10

Effect of different muscle shortening velocities during prolonged incremental cycling exercise on the plasma growth hormone, insulin, glucose, glucagon, cortisol, leptin and lactate concentrations

67%

Zoladz J. A., Duda K., Konturek S. J., Sliwowski Z., Pawlik T., Majerczak J.

Journal of Physiology and Pharmacology

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2002

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tom 53

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nr 3

Strenuous exercise was reported to involve the alteration in the release of some "stress" hormones such as growth hormone (GH), cortisol, catecholamines and appropriate adjustment of energy metabolism but the relative contribution of these hormones to metabolic response, to cycling exercise performed at different muscle shortening velocities, has not been clarified. Aims: The purpose of this experiment was to assess the effect of applying different pedalling rates during a prolonged incremental cycling exercise test on the changes in the plasma levels of growth hormone, cortisol, insulin, glucagon and leptin in humans. Material and Methods: Fifteen healthy non-smoking men (means ± SD: age 22.9 ± 2.4 years; body mass 71.9 ± 8.2 kg; height 178 ± 6 cm; with VO2max of 3.896 ± 0.544 l . min-1), assessed in laboratory tests, were subjects in this study. The subjects performed in two different days a prolonged incremental exercise tests at two different pedalling rates, one of them at 60 and another at 120 rev . min-1. During this tests the power output has increased by 30 W every 6 minutes. The tests were stopped when the subject reached about 70 % of the VO2max. Results and conclusions: We have found that choosing slow or fast pedalling rates (60 or 120 rev . min-1), while generating the same external mechanical power output, had no effect on the pattern of changes in plasma cortisol, insulin, glucagon, glucose and leptin concentrations. But, generation of the same external mechanical power output at 120 rev . min-1 causes more stepper increase (p < 0.01) in the plasma growth hormone concentration [GH]pl and plasma lactate concentrations [La]pl when compared to that observed during cycling at 60 rev . min-1. We have also found that the onset of a significant increase in [GH]pl during cycling at 60 rev . min-1 was not accompanied by significant increase in [La]pl. While during cycling at 120 rev . min-1 the onset of a significant increase in [La]pl occurred without increase in [GH]pl, but with continuation of exercise when plasma [La]pl increased, there was also a parallel rise in plasma [GH]pl, as reported before. This results indicates that the increase in [GH]pl during exercise is not closely related to the increase in [La]pl.

11

Wplyw mleczanow na jakosc mikrobiologiczna przechowywanych wedlin

59%

Smiechowicz I.

Żywność Technologia Jakość

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1997

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tom 04

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nr 1

52-60

12

Stezenie mleczanow i glukozy w surowicy krwi oraz glikogenu w watrobie w zatruciu ostrym chlorfenwinfosem

51%

Lukaszewicz-Hussain A., Chyczewski L., Moniuszko-Jakoniuk J.

Bromatologia i Chemia Toksykologiczna

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1998

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tom 31

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nr 3

251-258

Celem pracy było zbadanie wpływu chlorfenwinfosu, w dawce jednorazowej, na stężenie mleczanów i glukozy w surowicy krwi oraz na obraz glikogenu w wątrobie.

Ograniczanie wyników

High content of MyHC II in vastus lateralis is accompanied by higher VO2/power output ratio during moderate intensity cycling performed both at low and at high pedalling rates

Growth and antioxidant activity of Desulfotomaculum acetoxidans DSM 771 cultivated in acetate or lactate containing media

Step and ramp induction of myocardial ischemia: comparison of in vivo and in silico results

Evaluation of glycolytic potential usefulness for diagnosis of meat quality in pigs free of RYR1T and RN- alleles

Changes in lactate, pyruvate and glucose levels in field hockey players under different training and match stimuli

Lactate in cardiogenic shock - current understanding and clinical implications

Early effects of short-term endurance training on hormonal responses to graded exercise

Muscle biochemistry in rainbow trout Oncorhynchus mykiss following Yersinia ruckeri vaccination

Exercise-induced biochemical alterations in the blood of horses involved in recreational horseback riding in Pomeranian Region (Northern Poland)

Effect of different muscle shortening velocities during prolonged incremental cycling exercise on the plasma growth hormone, insulin, glucose, glucagon, cortisol, leptin and lactate concentrations

Wplyw mleczanow na jakosc mikrobiologiczna przechowywanych wedlin

Stezenie mleczanow i glukozy w surowicy krwi oraz glikogenu w watrobie w zatruciu ostrym chlorfenwinfosem