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1

Apoptoza w atrezji pecherzykow jajnikowych

100%
Blaszczyk B., Udala J., Gaczarzewicz D.
Medycyna Weterynaryjna
|
2000
|
tom 56
|
nr 03
158-162
A number of experiments have shown that only few among the follicles which start growing, are ovulable while the rest become atretic. In the regulation of atresia process primarily gonadotropins and steroids participate as well as many other growth factors. Recent studies have demonstrated that apoptosis of granulosa cells is one of the main factors responsible for follicular atresia. It is believed that in programmed granulosa cell death a decisive role is played by secondary messenger signal cells. It was also shown, that apoptosis of granulosa cells can be initiated by disturbances of metabolism cAMP. In the process this will reach to the expression of genes responsible for degenerative changes of a cell leading to its death. The course of apoptosis of granulosa cells is characterized by similar morphological and biochemical changes which accompany apoptosis of other types of cells. These changes constitute one of the processes initiating changes of structural ovarian follicles. Programmed death of granulosa cells is therefore another piece of evidence, that apoptosis is a physiological process regulating tissue regression and reconstruction.
2

Morfologia komórek ziarnistych pęcherzyków jajnikowych gęsi

100%
Wojtysiak D., Kapkowska E.
Zeszyty Naukowe. Przegląd Hodowlany
|
2001
|
tom 57
3

Ultrastruktura przedowulacyjnych pęcherzyków jajnikowych gęsi

86%
Wojtysiak D.
Zeszyty Naukowe. Przegląd Hodowlany
|
2000
|
tom 49
4

Wplyw komorek ziarnistych i komorek oslonki wewnetrznej na dojrzewanie in vitro oocytow klaczy

86%
Slonina D., Okolski A.
Medycyna Weterynaryjna
|
1993
|
tom 49
|
nr 07
324-326
The ovaries from 173 mares were collected at the time of slaughter from September 1991 to September 1992 and follicles (5—20 mm in diameter) were isolated and dissected to obtain oocytes for culture. 669 oocytes were subjected to one of four different culture treatments: Gr. 1-medium with oocytes, Gr. 2-medium plus 3 X 10⁶/ml granulosa cells plus oocytes, Gr. 3-medium plus 0.3 X 10⁶/ml theca interna cells plus oocytes, Gr. 4-medium plus granulosa and theca interna plus oocytes. The oocytes were cultured for 24, 30, 36, 42 and 48 hrs. After culture cumulus cells the expansion and stage of nuclear maturition was determined. The majority of oocytes reached full nuclear maturition after 30 hrs. The best results — full dispersion of cumulus cells in 96% and 97% of the oocytes and metaphase II in 75% and 77% of the oocytes — were obtained after 36 and 42 hrs’culture of oocytes with theca interna cells, respectively.
5

Wplyw DDT i jego metabolitow na czynnosc sekrecyjna komorek lutealnych, granulozy i endometrium krowy

72%
Wrobel M., Mlynarczuk J., Kotwica J.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 04B
583-587
Dichlorodiphenyltrichloroethane (DDT) is a persistent insecticide, recognized as an environmental pollutant. Due to its lipophilic properties, DDT and its metabolite (DDE) are accumulated in tissues of farm animals. The aim of the study was to examine the influence of DDT and DDE on estradiol, progesterone and oxytocin secretion from the ovary and on prostaglandin (F2α and E2) secretion from the uterus, was investigated. Granulosa, luteal and endometrial cells from cows at 8-12 days of the estrous cycle were treated for 24-72 h with 0.1-10 ng/ml of DDT, p,p’-DDE, o,p’-DDE or with a technical mixture of DDE isomers. Neither DDT nor DDE were found to affect the viability of cells compared to the control. They also did not affect the secretion of estradiol from granulosa cells. The utilized pollutants increased (P < 0.05-0.001) the progesterone and oxytocin secretion from luteal and granulosa cells. They also stimulated (P < 0.05) PGF2á secretion but simultaneously reduced (P < 0.001) PGE2 secretion from endometrial cells. Hence the ratio of PGF2α to PGE2 was markedly changed, from 1:1 in the control, up to 1:4-10 in treated cells. It has been concluded that DDT and its metabolites may impair regulation of the estrous cycle in cows by stimulation of oxytocin secretion from luteal and granulosa cells and by stimulation of PGF2α and the simultaneous inhibition of PGE2 secretion from endometrial cells.
6

Wpływ komórek ziarnistych i pożywek hodowlanych na kompetencje mejotyczne oocytów bydła oraz ich zdolność do rozwoju po zapłodnieniu in vitro

72%
Mlodawska W., Ploszaj E.
Medycyna Weterynaryjna
|
2013
|
tom 69
|
nr 05
The aim of the study was to compare the effect of gonadotropic hormones and granulosa cells on the maturation and developmental capacity of cattle oocytes in vitro, as well as the effect of TCM 199 and DMEM/F12 media on the development of embryos obtained in co-culture with oviduct epithelial cells. Fertilization was performed with the use of frozen semen from 2 bulls. Twenty hours after insemination, presumptive zygotes were placed in co-culture with oviduct cells in a TCM 199 (TCM-KJ co-culture) or a DMEM/F12 medium (DMEM-KJ co-culture) and cultured for 7-9 days. Metaphase II was reached by 40% and 48% of oocytes cultured in the presence of granulosa cells and gonadotropins, respectively. Only embryos obtained from oocytes maturing in the presence of granulosa cells developed to the blastocyst stage. Considerably more dividing embryos were obtained when the presumptive zygotes were co-cultured with TCM-KJ (38.1%) rather than with DMEM-KJ (8.6%; P < 0.01). This study showed that the presence of granulosa cells had no effect on the nuclear maturation of cattle oocytes, but increased their capacity for embryonic development. TCM 199 is much more useful than DMEM/F12 for the co-culture of cattle embryos with oviduct cells.
7

Udzial opioidow w lokalnej regulacji funkcji jajnika

72%
Przala J., Kaminski T., Okrasa S., Siawrys G., Bogacka I.
Postępy Biologii Komórki. Suplement
|
1999
|
tom 12
113-116
8

Wpływ leptyny na sekrecję estradiolu przez komórki ziarniste pęcherzyka jajnikowego owiec

72%
Murawski M., Krygowska K., Zieba D., Wierzchos E., Gertler A.
Roczniki Naukowe Zootechniki. Suplement
|
2003
|
tom 17
|
nr 1
9

Funkcja komorek ziarnistych wzgorka jajonosnego.

72%
Szoltys M.
Postępy Biologii Komórki. Suplement
|
1999
|
tom 12
189-192
10

Ekspansja komorek ziarnistych wzgorka jajonosnego - proces niezbedny do prawidlowego przebiegu owulacji i zaplodnienia

58%
Kotarska K.
Postępy Biologii Komórki
|
2009
|
tom 36
|
nr 2
171-187
11

Wplyw opioidow na steroidogeneze w komorkach ziarnistych i oslonki wewnetrznej pecherzykow jajnikowych swin; mechanizm dzialania agonisty opioidowego FK 33-824

58%
Kaminski T.
Postępy Biologii Komórki
|
2005
|
tom 32
|
nr 3
477-493
12

Ekspresja genow dla NP-I-OT i PGA w komorkach lutealnych i warstwy ziarnistej pecherzyka jajnikowego oraz czynnika LIF w komorkach endometrium krowy pod wplywem DDT i jego metabolitow

58%
Mlynarczuk J., Wrobel M., Kotwica J.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 04B
579-582
Ovarian oxytocin (OT) and endometrial leukemia inhibitory factor (LIF) are involved in estrous cycle regulation and implantation of the blastocyst in cows. For this reason the authors investigated the effect of DDT and its metabolites (DDE), known as environmental pollutants, on the expression of genes involved in OT and LIF synthesis. Granulosa from follicles (1 cm in diameter), luteal and endometrial cells from cows on days 8-12 of the estrous cycle were treated for 6 h with DDT, p,p’-DDE, o,p’-DDE and technical mixture (MIX) of DDE isomers (10 ng/ml each). Obtained RNA was reverse transcribed and cDNA was amplified by PCR using primers for genes of NP-I/OT, PGA, LIF and G3PDH as a reference gene. DDT and DDE in granulosa cells and DDE in luteal cells increased (P < 0.05) the expression of the NP-I/OT gene, while DDE in granulosa cells and MIX in luteal cells increased the expression of the PGA gene (P < 0.05). In contrast, p,p’-DDE and MIX reduced (P < 0.05) the LIF expression in endometrial cells. Obtained data allow the authors to assume that DDE and its metabolite impairs regulation of the estrous cycle, affecting OT and LIF synthesis on the genomic level.
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