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Modification of the ELISA for the determination of antibodies of IgG1, IgG2 and IgM classes in the serum of bovine leukaemia virus - infected animals

100%

Kozaczynska B.

Bulletin of the Veterinary Institute in Puławy

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1996

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tom 40

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nr 1

The aim of the experiments conducted was to modify the indirect ELISA to determine different immunoglobulin classes in the reaction of antibodies with bovine leukaemia virus. Rabbit sera immunized against bovine IgG₁, IgG₂ and IgM as well as serum conjugates were used in the modified method. Examinations of 100 reference serum samples from BLV-infected animals and 50 samples from BLV-free animals allowed us to establish the optimum dilution of the components used and the threshold optical densities of the test.

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The presence of interfering antibodies in selected control sera on the example of a set for determination of tPSA

84%

Hajdrowska B., Krzakiewicz K., Czubasiewicz Z., Strzelczyk J. K., Szewczyk M., Brus R.

Health Problems of Civilization

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2015

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tom 09

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nr 2

The human sera often contain substances which interfere in the course of immunochemical reactions. It is essential for the control sera used to determine concentrations of antigen of the prostate not to contain such ingredients. The aim of this study was to test the control sera, three producers, containing low (N) and high (P) concentrations of prostate antigen (tPSA). As a factor blocking the interfering actions, the TRU Bloc Meridian Life Science, Inc. reagent was used which contains antibodies against mouse protein and rheumatoid factor. The sera with a low concentration of tPSA demonstrated the presence of interfering substances.

3

Leptin measurement in urine is a reliable method of monitoring its secretion in patients with obstructive sleep apnea syndrome

84%

Chorostowska-Wynimko J., Plywaczewski R., Jonczak L., Bednarek M., Stepniewska A., Borowska A., Gorecka D.

Journal of Physiology and Pharmacology. Supplement

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2007

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tom 58

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nr 5

4

CD4 and CD8 cells in mice infected with Trichinella spiralis and treated with cyclosporine A

84%

Karmanska K., Houszka M., Mista D., Stefaniak E.

Wiadomości Parazytologiczne

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1994

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tom 40

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nr 4

Studies were undertaken to evaluate by parasitological techniques the course of intestinal and muscular invasions in mice infected with Trichinella spiralis and treated with TFX-Thymomodulin (TFX-Th). Studies were conducted on 120 mice of BALB/c and SWISS strains, infected each with a mean invasive dose of 300 T. spiralis larvae. TFX-Th (Thymoorgan GmbH Pharmazie Co. KG, Vienenburg, Germany) was administered subcutaneously at 30 mg/kg body weight. Depending upon duration of TFX-Th administration and stages of intestinal and muscular invasions, three experiments were distinguished in the study. The results indicated that TFX-Th promoted eradication of larvae from muscles, whether administered at the early of the late stage of T. spiralis invasion.

5

Immunoenzymatic method for progesterone detection in bovine urine, muscles and serum by means of a commercial EIA test used in human medicine

84%

Wozniak B., Wojton B.

Bulletin of the Veterinary Institute in Puławy

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1995

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tom 39

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nr 2

6

The cells observed inside capsules of larvae in the course of experimental trichinellosis in mice

84%

Karmanska K., Houszka M., Widyma A.

Wiadomości Parazytologiczne

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1997

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tom 43

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nr 3

In the course of trichinellosis in mice the numerous cells inside larval capsules were observed. Beginning on 14 day post infection (dpi) they were seen in about 7% of infected muscles fibers but at 21 dpi the percentage of capsules with the cells amounted to 77. In the next stage of infection the number of capsules containing cells continued to increase and reach almost 100% at 60 dpi. The identification of the cells was carried out by the indirect immunoenzymatic method using anti-CD4⁺, anti-CD8⁺ and anti-macrophage (Mac-1 antigen) monoclonal antibodies. In course of observation the level of CD4⁺ cells decreased from 11.3 to 0.7, of CD8⁺ from 17.7 to 3.0 and of macrophages from 71.0 to 23.1 %. The most numerous CD4⁺ cells were seen at 14 dpi while the CD8⁺ and macrophages at 21 dpi. Beginning on 28 dpi negatively reacting cells inside larval capsules were also observed. The number of these cells increased from 23.8 to 73.2% at 60 dpi and their identification requires further investigations. The role and the function of all these cells are discussed.

7

Oznaczanie zawartosci aflatoksyny B1 w paszach metoda immunoenzymatyczna - ocena testu RIDASCREEN aflatoxin B1

67%

Kozak A., Wisniewska-Dmytrow H., Zmudzki J.

Bromatologia i Chemia Toksykologiczna

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1995

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tom 28

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nr 4

383-387

Przedstawiono ocenę przydatności testu immunoenzymatycznego Ridascreen® Af- latoxin B, do oznaczania zawartości aflatoksyny B, w paszach. Wartość testu oceniono przeprowadzając równolegle analizy porównawcze badanych pasz zalecaną metodą chromatografii cieczowej. Przeprowadzona ocena statystyczna uzyskanych wyników świadczy o istotnej zgodności między obu metodami.

8

Borrelia burgdorferi infection among forestry workers - assessed with an immunoenzymatic method [ELISA], PCR, and correlated with the clinical state of the patients

67%

Niscigorska J., Skotarczak B., Wodecka B.

Annals of Agricultural and Environmental Medicine

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2003

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tom 10

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nr 1

Occurrence of borreliosis in human population is associated with possibility of contact with the biological vector of this disease - a common European tick, Ixodes ricinus. Therefore, the highest number of cases of Lyme disease has been recorded among forestry workers and inhabitants of wooded areas. Diagnostics of borreliosis is based on immunoserologic tests - ELISA or indirect immunofluorescence method, Western blot technique, or on increasingly popular DNA examination using the polymerase chain reaction (PCR). In the present study, where 61% of the forestry workers were seropositive, we also tried to find a correlation between the results of serological tests and PCR tests with the clinical state of the patients. Despite finding IgM antibodies in 10 persons tested, which would indicate their recent infection, no DNA of B. burgdorferi s.l. was detected in their blood. Also, no DNA of this bacteria was present in 8 persons with IgM and IgG antibodies. No genetic material of the bacteria was found in persons with IgG antibodies, indicating the possibility of chronic infection. The clinical data suggested past symptomatic infection (ECM), or even more often, asymptomatic infection with B. burgdorferi.

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Oznaczanie gliadyny w niektorych produktach bezglutenowych metoda Elisa

59%

Maslowska J., Leszczynska J.

Roczniki Państwowego Zakładu Higieny

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1992

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tom 43

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nr 1

61-66

Zastosowano z pomyślnym rezultatem metodę immunoenzymatyczną z wykorzystaniem króliczych przeciwciał przeciwko gliadynie i koniugatów przeciwciał z peroksydazą do oznaczania gliadyny w bezglutenowej żywności. Otrzymane wyniki oznaczania gliadyny wskazują, że zawartość gliadyny jest w nich niska i nie przekracza 0,1%.

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Zastosowanie metod prognozowania mikrobiologicznego do okreslania rozwoju mikroflory saprofitycznej w produktach miesnych utrwalonych lizozymem w formie monomeru

59%

Rosiak E., Kolozyn-Krajewska D.

Żywność Nauka Technologia Jakość

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2003

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tom 10

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nr 3

5-20

Celem pracy było opracowanie matematycznych modeli wzrostu ogólnej liczby drobnoustrojów oraz saprofitycznych bakterii z rodzaju Pseudomonas spp. w modelowych produktach mięsnych utrwalonych lizozymem w formie monomeru. Badania mikrobiologiczne wykonano klasycznymi metodami płytkowymi. Do uzyskanych danych empirycznych dopasowano funkcje s-kształtne (Gompertza i logistyczną) oraz funkcje wielomianowe stopnia drugiego i trzeciego. Szacowania parametrów funkcji pierwszorzędowych dokonano z wykorzystaniem procedur iteracyjnych, wyznaczających najmniejszą wartość sumy kwadratów różnicy błędu pomiędzy wartościami empirycznymi i teoretycznymi. Szacowania parametrów modeli zbiorczych dokonano klasyczną metodą najmniejszych kwadratów. Na podstawie modeli pierwszorzędowych uzyskano parametry kinetyczne opisujące rozwój badanych grup drobnoustrojów. Modele zbiorcze pozwoliły określić wpływ badanych czynników środowiskowych na populację drobnoustrojów.

Ograniczanie wyników

Modification of the ELISA for the determination of antibodies of IgG1, IgG2 and IgM classes in the serum of bovine leukaemia virus - infected animals

The presence of interfering antibodies in selected control sera on the example of a set for determination of tPSA

Leptin measurement in urine is a reliable method of monitoring its secretion in patients with obstructive sleep apnea syndrome

CD4 and CD8 cells in mice infected with Trichinella spiralis and treated with cyclosporine A

Immunoenzymatic method for progesterone detection in bovine urine, muscles and serum by means of a commercial EIA test used in human medicine

The cells observed inside capsules of larvae in the course of experimental trichinellosis in mice

Oznaczanie zawartosci aflatoksyny B1 w paszach metoda immunoenzymatyczna - ocena testu RIDASCREEN aflatoxin B1

Borrelia burgdorferi infection among forestry workers - assessed with an immunoenzymatic method [ELISA], PCR, and correlated with the clinical state of the patients

Oznaczanie gliadyny w niektorych produktach bezglutenowych metoda Elisa

Zastosowanie metod prognozowania mikrobiologicznego do okreslania rozwoju mikroflory saprofitycznej w produktach miesnych utrwalonych lizozymem w formie monomeru