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The concentration and composition of fungal flora in dental unit waterlines (DUWL) were evaluated. For this purpose, water samples from unit reservoirs and high-speed handpieces, and biofilm samples from the waterline walls from units were collected. Subsequently, analogous samples from DUWL were taken before and after disinfection using agent containing hydrogen peroxide. In the examined samples, the yeast-like fungi Candida albicans, Candida curvata and Geotrichum candidum were found. The following species of mould were also identified: Aspergillus amstelodami, Aspergillus fumigatus, Aspergillus glaucus group, Aspergillus (=Eurotium herbariorum) repens, Citromyces spp., Penicillium (glabrum) frequentans, Penicillium pusillum, Penicillium turolense and Sclerotium sclerotiorum (Sclerotinia sclerotiorum) . Before disinfection, Candida curvata and Candida albicans constituted the greatest proportion of the total fungi in the reservoirs water; in the water of handpieces - Candida albicans and Aspergillus glaucus group; and in the biofilm samples - Aspergillus glaucus group and Candida albicans. After disinfection, in all 3 kinds of samples, Candida albicans prevailed, constituting from 31.2-85.7% of the total fungi. The application of agent containing hydrogen peroxide caused a significant decrease both in the number of total fungi and individual fungal species, which confirms the product effectiveness in fungal decontamination of DUWL.
Mycological analyses were carried out on air samples collected with the sedimentation and impactor methods. Fungi were assayed in atmospheric air sampled in the city centre of Olsztyn and in the forest at Lake Kortowskie. The experiment aimed at evaluating the contamination of atmospheric air with fungal microflora in the city centre of Olsztyn (3 stations) and in the forest at Lake Kortowskie (one station), collected with the sedimentation and impactor methods. Differentiated population numbers of fungi, reported in the atmospheric air of the city of Olsztyn, appeared to depend, to a great extent, on meteorological conditions.
Maize attacked by the facultative biotrophic smut pathogen, Ustilago maydis (Basidomycetes) was ensiled in microsilos in two combinations of infected and non-infected plants, with or without biological (Polmasil) or chemical (Kemisile 2000) additives. The silage was subjected to chemical and microbiological analyses. The aerobic stability of the silage was tested. Ustilago maydis constituted up to 6.22% of the total yeast content (CFU g-1) in the infected and 0.22% in the noninfected material. Silage made from infected plants had a higher content of fungi (6.45 vs 4.54), moulds (6.20 vs 4.54), and yeast (6.08 vs 3.71) expressed as log10 CFU g-1 as compared with noninfected plants. The use of the chemical additive decreased these effects. The contents of ochratoxin, zearalenone, and deoxynivalenol were low and did not change during the ensiling. The cytotoxicity test did reveal toxicity of silage from infected plants, however. It seems that the observed toxicity is the effect of toxins other than those assayed.
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