The objective of this study was to determine the influence of biological silage additive (Bonsilage) on the hygiene quality and nutritive value of maize and grass-legume silages. The experiments were conducted on FAO 240 maize (Zea mays L.) and a mixture of italian ryegrass (Lolium multiflorum L.), 50% with alfalfa (Medicago media Pers.), 50%. Group 1 was a control and comprised silage without any additives, group 2 was ensiled with the addition of 4 cm3 kg-1 biological silage additive. After 60 days of silage process individual silages were subjected to microbiological composition, and chemical analyses of silages were also determined. Similar analyses were repeated at day 7 following exposure to oxygen. The applied biological silage additive was found to reduce (P<0.05) numbers of Clostridium, Enterobacteriaceae, yeasts and mold fungi cells, and increase (P<0.05) the number of LAB (lactic acid bacteria) in comparison with the control in both silages. Chemical analysis of the maize silage showed that the biological additive caused an increase (P<0.05) in DM (dry matter), CP (crude protein), WSC (water soluble carbohydrates), LA (lactic acid), AA (acetic acid), ethanol, and a decrease (P<0.05) in the concentration of BA (butyric acid), N-NH3 and pH value in comparison with the control. Chemical analysis of silage samples from the grass-legume mixture showed that the additive caused an increase (P<0.05) in the content of DM, CP, WSC, LA and AA in comparison with the control. Samples of silage with the addition of an inoculant were characterized by a lower (P<0.05) content of BA, N-NH3, ethanol and pH value. The biological additive impoved the aerobic stability of silages in the aerobic phase.
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