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1

Simultaneous SSCP genotyping of coding and regulatory fragments of bovine beta-lactoglobulin gene

100%
Kaminski S.
Natural Sciences
|
2000
|
tom 04
2

Effects of heating and pressurization on foaming properties of beta-lactoglobulin

75%
Leman J., Dolgan T.
Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology
|
2004
|
tom 07
|
nr 2
The effects of heating (80oC/10 min and 90oC/2 min) and pressurization (300, 600 and 900 MPa for 10 min and 300 MPa for 10 to 30 min) on foaming properties of beta-lactoglobulin (beta-Lg) obtained from whey retentate in mild conditions were compared in the pH range of 5.0 to 7.0. Generally, heating and pressurization improved the beta-Lg foamability, except that at pH 7.0 (1) heating of the protein worsened (80oC/10 min) the foaming properties or not changing (90oC/2 min) the foam capacity - improved the foam stability and (2) pressurization at above 300 MPa drastically worsened the foam stability (600 MPa) or caused that the foam was unstable (900 MPa). The highest foam capacity had beta-Lg pressurized for 10 min at 300 MPa in pH 7.0 and lengthening of the pressurization time up to 30 min resulted in the increase in the foam stability with the foam capacity being practically unchanged.
3

Beta-lactoglobulin genetic variants in Simmental breed and its association with compositional properties and rennetability of milk

63%
Michalcova A., Krupova Z., Bulla J.
Prace i Materiały Zootechniczne
|
2009
|
tom 67
The protein composition and technological traits were investigated in Simentaler milk with genetic variants A or B of ß-lactoglobulin (ß-LG). Data were included from 78 cows from 3 dairy farms. Genotypes of ß-LG were determined by applying horizontal starch-gel electrophoresis with addition of urea and mercaptoethanol. In addition, fat, crude protein (CP), true protein (TP), casein (CN), whey protein (WP), non-protein nitrogen (NPN), lactose and calcium (Ca) content, casein number (CNno) and rennetability of milk from Slimental breed were examinated. The method of last squares means using the general linear model (GLM) was used for statistical analysis of data. The model included heard and genotypes of ß-LG as fixed effects. The genotypes of ß-LG had no significant association with fat, CP and TP content, while CN and WP content and CNno were significantly affected by the genotypes of ß-LG. The BB genotype of ß-LG was significantly associated with higher CN content and CNno. The positive effect of the allele B of ß-LG on rennetability was found out.
4

Reduction of milk allergenicity using new synthesized Cu(II) complex as artificial protease

63%
Divsalar A., Saboury A. A., Mansouri-Torshizi H.
Polish Journal of Food and Nutrition Sciences
|
2011
|
tom 61
|
nr Suppl.1
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effect of cow's breed and feeding season on the content of bioactive whey protein of milk produced according to principles of the biodynamic farming

63%
Kuczynska B. A., Puppel K. S., Metera E., Sakowski T., Kapusta A., Budzinski A., Grodzki H.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2013
|
tom 52
The aim of this study was to determine genetic effect of cow breed and feeding season on content of bioactive whey protein of cow's milk. In the selected biodynamic dairy farm, cows of two different breeds, i.e. Brown Swiss (BS) and Holstein-Friesian black and white (HF) were kept under the same environment conditions. The samples of milk were collected twice (in summer and winter) from 30 cows (in the same for number from BS and HF). Content of following whey protein: P-lactoglobuline (P-LG), a-lactoalbumine (a-LA), lactoferrin (Lf), lacto-peroxidase (Lp), lisozyme (Lz) and bovine serum albumine (BSA) were determined used RP-HPLC technique. The studies showed a significant effect of breed and feeding season on the content of whey proteins in milk. The average content of whey proteins in milk of cows ranged from 0.73% for the HF breed in winter to 0.84% for the BS breed in summer. Important differences weren't stated in the content of lactoferrin and lactopero-xidase in the milk, and for her the content fluctuated from 0.201 to 0.259 g/l and from 0.111 to 0.154 g/l appropriately at examined breeds. There were no statistically significant differences in the level of a-lactoalbumine in the milk of cows. Its content was ranged from 1.663 to 1.994 g/l. Statistically important differences were shown in conducted experience for the breeds and the feeding season (P < 0.01) for the content of P-lacto-globuline from BS cows in the period of summer feeding was 5.38 g/l, however reduced to the level herself by the winter 4.62 g/l. The similar trend was shown in the milk of HF cows in the summer season the concentration of this white was 4.60 g/l, and in the winter season underwent lowering up to 4.41 g/l. The experiment demonstrated statistically significant differences for the breed and feeding season for the content of P-lactoglobuline in cow's milk.
6

Polymorphism of milk beta-lactoglobulin and its effect on milk yield and reproductive traits of Simmental cows

63%
Felenczak A., Jezowit-Jurek M., Gil Z., Adamczyk K.
Annals of Animal Science
|
2008
|
tom 08
|
nr 3
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Genotyping of bovine beta-lactoglobulin [LGB] by PCR-SSCP technique

63%
Kaminski S., Zabolewicz T.
Journal of Applied Genetics
|
1997
|
tom 38
|
nr 4
A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

SSCP polymorphism within 5' region of bovine lactoglobulin [LGB] gene

63%
Kaminski S., Zabolewicz T.
Journal of Applied Genetics
|
1998
|
tom 39
|
nr 1
In the paper the detection of the SSCP polymorphism within the 5’ fragment of bovine beta-lactoglobulin (LGB) gene is described. The 5’ fragment of LGB gene (209 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 124 animals (50 cows and 74 bulls) six SSCP patterns were identified and named Rl, R2, R3, R4, R5 and R6, which occured with the frequency of 0.32, 0.51, 0.09, 0.06, 0.01 and 0.01, respectively. The PCR-SSCP method is simple, fast, and relatively inexpensive. The SSCP polymorphism reported in the paper may be useful in looking for the associations between different SSCP patterns and LGB gene expression and milk properties.
9

Microstructure of gels made from intact and partially hydrolysed beta-lactoglobulin - methodological aspects

63%
Otte J., Ipsen R., Pedersen P. S., Wium H., Qvist K. B.
Polish Journal of Food and Nutrition Sciences
|
1998
|
tom 07
|
nr 2 Suppl.
10

Polymorphism of beta-lactoglobulin gene in Indian goats and its effect on milk yield

63%
Kumar A., Rout P. K., Roy R.
Journal of Applied Genetics
|
2006
|
tom 47
|
nr 1
Polymorphism in the β-LG gene in the Indian goat was investigated by the SDS-PAGE and PCR-RFLP method. SDS-PAGE was carried out in 1098 samples belonging to 8 different breeds of Indian goats. The electrophoretic pattern in the β-LG locus showed the presence of AA and AB genotypes with frequencies of 0.81 and 0.19,0.89 and 0.11, 0.50 and 0.50, 0.80 and 0.20, 0.84 and 0.16, 1.00 and 0.00, 0.98 and 0.02 and 0.950 and 0.050 in Jamunapari, Barbari, Marwari, Sirohi, Jakhrana, Beetal, Local UP and Local MP goats, respectively. A total of 358 individuals belonging to 13 different genetic groups were analyzed by the PCR-RFLP method. The amplified product was observed as 426 bp and the restriction digestion with SacII revealed three genotypes, namely S₁S₁, S₁S₂ and S₂S₂ at the β-LG locus. The frequency of the S₂S₂ genotype ranged from 0.42 to 1.00 in the population. An analysis was carried out in Jamunapari and Barbari goats to observe the effect of the β-LG genotype on 90-day milk yield. Least squares analysis of data showed that β-LG AA animals had higher milk yield than the β-LG AB genotype in both breeds (P < 0.01).
11

An association of growth hormone, kappa-casein, beta-lactoglobulin, leptin and Pit-1 loci polymorphism with growth rate and carcass traits in beef cattle

63%
Zwierzchowski L., Oprzadek J., Dymnicki E., Dzierzbicki P.
Animal Science Papers and Reports
|
2001
|
tom 19
|
nr 1
12

Studies upon genetic and environmental factors affecting the cholesterol content of cow milk. I. Relationship between the polymorphic form of beta-lactoglobulin, somatic cell count, cow age and stage of lactation and cholesterol content of milk

51%
Strzalkowska N., Jozwik A., Bagnicka E., Krzyzewski J., Horbanczuk J. O.
Animal Science Papers and Reports
|
2009
|
tom 27
|
nr 2
95-103
Despite the fact that cholesterol is a comparatively stable component of cows’ milk its concentration is, within a certain range, subject to significant variation related to the season (probably the feeding system), lactation stage and somatic cell count in milk. The highest differences (about 25%) in the amount of cholesterol per g milk fat were observed between the first and last lactation stage. Despite the decreasing milk yield with the progress of lactation, the amount of cholesterol secreted with milk increased significantly. In the milk of cows for which the somatic cell count was below 100 thousand/ml the cholesterol content was by about 10% lower than that in milk characterized by a higher somatic cell count.The positive correlation coefficients obtained between the amount of cholesterol expressed as mg/100 ml milk and the per cent of fat and protein indicate that selection conducted for increasing the concentration of nutritive components in milk will result in an increased cholesterol content.However, the quantity of cholesterol per 1 g milk fat will decrease. There was observed no correlation between the content of cholesterol in milk and the polymorphic forms of LGB.
13

Zaleznosc pomiedzy polimorfizmem beta-laktoglobuliny a produkcyjnoscia mleczna krow rasy hf

51%
Czerniawska-Piatkowska E., Szewczuk M., Zych S.
Acta Scientiarum Polonorum. Zootechnica
|
2007
|
tom 06
|
nr 1
23-27
14

Influence of manufacturing technology on amino acid composition of soft white unripened cheese from sheep milk

51%
Pakulski T., Bagnicka E.
Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology
|
2017
|
tom 20
|
nr 1
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Association between beta-lactoglobulin (LGB) polymorphism and yield and composition of milk Holstein-Friesian cows imported from Sweden

51%
Czerniawska-Piatkowska E., Szewczuk M., Olszewska A., Chocilowicz E.
Acta Scientiarum Polonorum. Zootechnica
|
2011
|
tom 10
|
nr 2
The study involved 102 Holstein-Friesian cows imported from Sweden, kept on a farm in the West Pomerania Province. Beta-lactoglobulin (LGB) genotypes were determined using PCR (Polymerase Chain Reaction) method according to Medrano andAquilar-Cordova [1990]. It was found that cows with the LGB AA genotype had the highest milk yield in all analysed 305-day lactations. The differences were significant in the third lactation (P≤0.01, P≤0.05). For the same genotype, the highest milk protein yield and content for the three analysed lactations were recorded. The highest milk fat content and yield were found in the BB homozygotes for all the analysed lactations.
16

Effects of kappa-casein and beta-lactoglobulin loci polymorphism, cows' age, stage of lactation and somatic cell count on daily milk yield and milk composition in Polish Black-and-White cattle

51%
Strzalkowska N., Krzyzewski J., Zwierzchowski L., Ryniewicz Z.
Animal Science Papers and Reports
|
2002
|
tom 20
|
nr 1
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Mozliwosci oznaczania zmian denaturacyjnych alfa-la mleka krowiego

45%
Mierzejewska D., Jedrychowski L.
Żywność Nauka Technologia Jakość
|
1999
|
tom 06
|
nr 3
36-47
Rozbudowana struktura drugo- i trzeciorzędowa białek serwatkowych: α-la, β-lg, BSA i immunoglobulin, sprawia, że są podatne na procesy denaturacyjne wywoływane czynnikami chemicznymi lub fizycznymi (temperatura czy wysokie ciśnienia). Procesy termiczne, szeroko stosowane w przemyśle mleczarskim, powodują denaturację, zmianę właściwości fizyko-chemicznych i żywieniowych białek serwatkowych. Poznanie tych zmian i technik, którymi można je obserwować jest istotne ze względów badawczych i żywieniowych. W artykule przedstawiono przegląd kilku technik wykorzystujących zmianę właściwości immunore-aktywnych, termodynamicznych (temperatura i entalpia denaturacji, energia aktywacji), fizykochemicznych pozwalających lepiej poznać proces denaturacji tych białek.
18

Zwiazek miedzy polimorfizmem bialek mleka i zroznicowaniem wydajnosci oraz skladu mleka krow utrzymywanych w stadzie bydla rasy czarno-bialej Kombinatu Rolnego Kietrz

38%
Ziemianski R., Juszczak J., Czarnik U., Cwikla A., Zabolewicz T., Walawski K.
Acta Scientiarum Polonorum. Zootechnica
|
2005
|
tom 04
|
nr 1
163-170
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