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We assessed the bioflocculant production potentials of a consortium of two marine bacterial species belonging to the Oceanobacillus and Halobacillus genera, isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa. Cell free culture broth of the consortium had a flocculating activity of 98.3%, which is higher than those of pure cultures of the individual species. The purified bioflocculant was more efficient (optimum dose 0.2 mg·ml⁻¹) in the flocculation of kaolin suspension (4 g·l⁻¹) compared to polyelectrolyte (optimum dose 0.3 mg·ml⁻¹) and alum (optimum dose 1 mg·ml⁻¹), which are both commercially available coagulants. A neutral pH condition and the presence of Ca²⁺ as cation resulted in optimum activity of the bioflocculant. Also, the purified bioflocculant removed chemical oxygen demand (COD) in brewery wastewater, dairy wastewater, and river water at efficiencies of 99.7, 99.9, and 63.5%, respectively, and also reduced their turbidity by 93.9, 88.3, and 98.6%, respectively. Composition analysis revealed the bioflocculant to be mainly polysaccharide with an amorphous-crystal-like structure. FTIR spectra revealed the presence of carboxyl, hydroxyl, and amino groups in its thermo-stability test, suggesting a thermostable bioflocculant.
The aim of this study was to identify the most common bacteria that may be found in the blood or on the feathers of white stork (Ciconia ciconia) chicks and predict their pathogenic potential for birds and humans. A variety of microorganisms, which include Staphylococcus aureus, Staphylococcus vitulinus, and Pseudomonas sp. were found. Based on breeding population densities and reproductive success over the past 25 years, we found no apparent effects of bacterial infections on the white stork population in Poland.
Campylobacter spp. is one of the main etiological factors of gastrointestinal diseases in people manifesting as alimentary infections. The microorganism is isolated 3-4 times more frequently in case of alimentary infections that other enteropathogenes, i.e. Salmonella or E. coli. Campylobacter spp. is a component of ordinary intestinal microflora in many animal species, including slaughter animals. Birds are one of the most important reservoirs of Campylobacter spp. With a relatively high internal body temperature at around 42℃, they are the appropriate environment for those bacteria, which show special thermal requirements. Wide presence of Campylobacter spp. in animal population causes the risk of contamination of food products such as raw meat and milk as well as water.
Probiotics have antibacterial effects against pathogenic bacteria in the gut while maintaining the balance of intestinal flora such as Lactobacillus. This study aimed to evaluate the antimicrobial activity of four Lactobacillus species against intestinal pathogenic. Four different species of Lactobacillus (Lactobacillus bulgaricus (PTCC 1332), Lactobacillus casei (PTCC 1608), Lactobacillus plantarum (PTCC 1058) and Lactobacillus Fermentum (PTCC 1638)) were experimented to investigate the inhibitory activity against 4 bacterial enteric pathogens (Escherichia coli, Staphylococcus aureus, Shigella dysenteriae and Salmonella paratyphi A) which were separately inoculated in MRS medium (de Man, Rogosa and Sharpe medium) for 48 hours at 37 °C and pH 7. Our results showed that enteropathogens growth was stopped in the presence of all Lactobacillus and inhibition zone was between 12 and 32 millimeter. It can be concluded that these four Lactobacillus strains had potential antimicrobial compounds against human enteric pathogens and should be further studied for their human health benefits.
The objective of the study was to identify an association between the genotypes of BoLA-DRB3.2 loci (*16/- and *23/-) and occurrence of sub-clinical mastitis or latent mammary gland infections caused by different bacterial species in dairy cows. Blood was withdrawn from of 275 cows from two Polish dairy herds, and animals were genotyped for BoLA-DRB3.2*16 and BoLA-DRB3.2*23 using MPTPCR technique. The bacteriological status of the mammary gland was determined by collecting and culturing composite milk samples. SCC was recorded by Fossomatic device after bacteriological examination. No relationship was identified between cows of *16/- or *23/- Bola-DRB3.2 genotype In susceptibility/resistance to sub-clinical mastitis caused by Staphylococcus aureus. However, genotype *23/- was found to be associated (P≤0.01) with increased susceptibility to sub-clinical mastitis caused by Streptococcus dysgalactiae.
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