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1

The physiological significance of the alternative pathways of angiotensin II production

100%

Kramkowski K., Mogielnicki A., Buczko W.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 4

Although the use of angiotensin converting enzyme inhibitors (ACE-Is) in clinical practice brought the great chance to recognize the RAS role in the physiology and pathology, there are still many questions which we cannot answer. This article reviews actually known pathways of angiotensin II (Ang II) and other peptides of renin-angiotensin system (RAS) production and their physiological significance. The various carboxy- and aminopeptidases generate a range of peptides, like Ang II, Ang III, Ang IV, Ang-(1-7) and Ang-(1-9) possessing their own and known biological activity. In this issue especially the alternative pathways of Ang II synthesis involving enzymes other than angiotensin-converting enzyme (ACE) are discussed. We present many evidences for the significance of a new pathway of Ang II production. It has been clearly shown that Ang I may be converted to Ang-(1-9) by angiotensin-converting enzyme-related carboxypeptidase (ACE-2) and then into Ang II in some tissues, but the enzymes responsible for this process are unknown till now. Although there are many data proving the existence of alternative pathways of Ang II production, we can still block only ACE and angiotensin receptor 1 (AT1) in clinical practice. It seems that a lot needs to be done before we can wildly complexively control RAS and treat more effectively cardiovascular disorders such as hypertension or heart failure.

2

Wplyw roznych czynnikow na poziom aktywnosci aminopeptydazowej u bakterii Pseudomonas sp.

72%

Jankiewicz U., Sepowicz M., Sawicka D.

Acta Scientiarum Polonorum. Biotechnologia

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2005

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tom 04

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nr 1-2

3-12

3

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The effect of feeding on aminopeptidase and non-specific esterase activity in the digestive system of pike-perch (Sander lucioperca L.)

72%

Kamaszewski M., Ostaszewska T.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2013

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tom 52

The pike-perch (Sander lucioperca L.) at the age of 18 days were fed for 21 days using three different diets: Agio Norse (An), casein-gelatin (Cas), cod meal with gelatin (Mac) and nauplius Artemia salina (Art - control diet). On the last day of the experiment, fish fed Art and An diets had the statistically significant highest body mass, length, and survival. On the last day, the highest aminopeptidase activity in the anterior intestine and posterior intestine was registered in fish fed nauplius Artemia salina. The lowest activity of this enzyme in the anterior intestine was to be found in fish fed with Cas diet, while there was no difference between among groups in the posterior intestine. The non-specific esterase activity was registered in the stomach, liver, anterior intestine as well as in the posterior intestine. The lowest activity of this enzyme in the stomach was observed in the pike-perch fed with the Cas diet. In the anterior intestine, the highest activity was registered in fish fed with Art, and the lowest - on the Cas and Mac diet. The results of the current research prove that feeding the pike-perch An diet has a positive effect of the survival of the pike-perch, their growth rate and the activity of the enzymes. By contrast, feeding pike-perch Cas and Mac diets did not satisfy nutritional needs of fish, resulting in their low survival, growth rate and low activity of the enzymes examined.

4

Microbial enzymatic activity and its relation to organic matter abundance on sheltered and exposed beaches on the Polish coast

72%

Astel A. M., Bigus K., Stec M.

Oceanologia

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2018

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tom 60

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nr 3

5

Ability of Pseudomonas sp. to synthesize aminopeptidases in the presence of various carbon and nitrogen sources

72%

Jankiewicz U., Bielawski W.

Acta Microbiologica Polonica

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2002

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tom 51

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nr 3

6

The molecular and biochemical characteristics of proline iminopeptidase from rye seedlings [Secale cereale L.]

72%

Szawlowska U., Prus W., Bielawski W.

Acta Physiologiae Plantarum

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2006

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tom 28

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nr 6

A proline iminopeptidase (EC. 3.4.11.5) was isolated from shoots of 3 day old seedlings. The purification procedure consisted of 5 steps: acid precipitation, gel filtration on Sephadex G-200, ion-exchange chromatography on Sepharose CL 6B, twice repeated hydrophobic chromatography on Phenyl-Sepharose HP. The enzyme was purified 404.8-fold, with the specific activity of 8.5 units·mg⁻¹ of protein with recovery yield of 3 %. The purified enzyme had a molecular mass of 225 kDa estimated by gel filtration and 55.4 kDa by SDS PAGE. This indicates that native enzyme is composed of four subunits. The enzyme was specific for proline β-naphtylamide among various amino acid β-naphtylamides. An optimal activity was observed at 37 °C at pH 7.75. The enzyme was thermostable up to 37 °C for 30 min. The enzyme was strongly inhibited by pHMB, E-64, heavy metal ions and partially by PMSF, DFP. The results suggest that cysteine and serine residues may participate in the enzyme activity.

7

Responses of mammary amino acid metabolism and aminopeptidase N gene expression to duodenal soybean small peptides and infusion of free amino acids in lactating goats

58%

Liu H., Wang L., Wang L.-B., Li S.-L., Cao Z.-J.

Journal of Animal and Feed Sciences

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2010

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tom 19

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nr 1

24-36

The duodenal perfusion technique was used to determine whether small peptides were more effective than free amino acids (FAA) for milk protein synthesis and to investigate the effects of infusing soyabean small peptides (SSP) or their FAA on the mammary amino acid (AA) metabolism of lactating goats. Six Saanen goats with silicon catheters implanted into their carotid artery and mammary veins were used in a Latin square design. Blood samples were collected from the carotid artery and mammary vein. The AA concentrations in the plasma, as well as mammary AA uptakes were monitored. The results showed that the concentrations of most essential amino acids (EAA) were significantly higher (P<0.05) in the FAA infusion treatment as compared with the SSP treatment, except for Val, Ile, and Met. The concentration of most of the non-essential amino acids (NEAA), measured after FAA infusion, was highest (P<0.05) when compared with the SSP treatment and the controls. The mammary uptake of most of EAA in the FAA infusion group was higher in comparison with the SSP infusion group. The mammary uptake to milk output ratios of EAA (except for Met) were low in the SSP treatment, compared with the FAA treatment. Significant increases in milk protein yield and milk protein content in the FAA infusion treatment were observed (P<0.05). In the SSP infusion treatment, milk yield and milk protein were numerically increased compared with the control. The abundance of aminopeptidase N (APN) mRNA in mammary tissue was 38.28 in the SSP infusion group, and 2.83, 5.28 in the control and FAA infusion treatment, respectively. In conclusion, the data suggest the FAA was more effective for milk protein synthesis, while the activity of APN was related to utilization of small peptides by the mammary gland.

8

Microbial ectoenzyme activity: useful parameters for characterizing the trophic conditions of lakes

58%

Kiersztyn B., Siuda W., Chrost R. J.

Polish Journal of Environmental Studies

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2002

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tom 11

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nr 4

This report describes the relationship between microbial enzymatic activities (aminopeptidase, esterase, and alkaline phosphatase) and the trophic state index of the studied lakes. Pelagic surface water samples were collected from nineteen lakes (Mazurian Lake District, northeastern Poland), characterized by different degrees of eutrophication, during spring homothermy and summer thermal stratification periods in 1999 and 2000. Aminopeptidase and esterase activities of microbial assemblages in unfiltered water samples were positively proportional to the trophic conditions of the studied lakes and both enzymes significantly correlated with Carlson's trophic state index of lakes. No correlation between alkaline phosphatase activities and the trophic state index of the studied lakes was found. This study showed that the selected parameters of microbial activity are very useful for the rapid determination of actual trophic conditions in lake ecosystems.

9

The properties and functions of bacterial aminopeptidases

58%

Jankiewicz U., Bielawski W.

Acta Microbiologica Polonica

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2003

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tom 52

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nr 3

10

Some examples of the use of electrophoretic protein analysis in taxonomic investigations of leguminous plants

44%

Przybylska J.

Journal of Applied Genetics

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1995

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tom 36

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nr 3

The article reviews the work of the author and coworkers on the use of electrophoretic protein analysis in taxonomie studies and classification of genetic resources of some leguminous plants: the genus Pisum, the Old-World Lupinus species and the cultivated Phaseolus species. On the example of the genus Pisum, the object of many years' investigations, the usefulness of electrophoretic analysis of different protein types - seed globulins, seed albumins and enzyme systems - is critically discussed. It has been concluded that variation in seed albumins, proteins often ignored in comparative analyses, should be more widely considered in taxonomie investigations. The conclusion was supported by the results obtained for Lupinus and Phaseolus species.

Ograniczanie wyników

The physiological significance of the alternative pathways of angiotensin II production

Wplyw roznych czynnikow na poziom aktywnosci aminopeptydazowej u bakterii Pseudomonas sp.

The effect of feeding on aminopeptidase and non-specific esterase activity in the digestive system of pike-perch (Sander lucioperca L.)

Microbial enzymatic activity and its relation to organic matter abundance on sheltered and exposed beaches on the Polish coast

Ability of Pseudomonas sp. to synthesize aminopeptidases in the presence of various carbon and nitrogen sources

The molecular and biochemical characteristics of proline iminopeptidase from rye seedlings [Secale cereale L.]

Responses of mammary amino acid metabolism and aminopeptidase N gene expression to duodenal soybean small peptides and infusion of free amino acids in lactating goats

Microbial ectoenzyme activity: useful parameters for characterizing the trophic conditions of lakes

The properties and functions of bacterial aminopeptidases

Some examples of the use of electrophoretic protein analysis in taxonomic investigations of leguminous plants