The fatty acid composition of milk from Lowland Black-and-White and Polish Red cows was determined for the period of pasture feeding. The total fatty acid profile was determined by gas chromatography with a 50 m long polar capillary CP Sil 88 column. The content of trans C18:1 acid was determined with the same column after separating first the fraction of trans monoenoic methyl esters by silver-ion thin-layer chromatography. The milk fat from Lowland Black-and-White cows was found to have significantly higher proportion of long-chain saturated fatty acids as compared with milk fat from Polish Red cows for the same feeding conditions. The proportion of monoenoic acids, including the isomers of trans-18:1 acid and polyenoic acids, was significantly higher in milk fat from Polish Red cows than Lowland Black-and-White cows.
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