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Gall-inducing insects may cause multiple physiological changes in host plants, such as the loss of photosynthetic pigments and reduced photosynthetic capacity. However, the direction of these changes is usually insect species-dependent. Therefore, the objective of this research was to characterize the indirect effects of galls induced by asexual generation of Neuroterus numismalis (Fourc.) and N. quercusbaccarum L. on photosynthesis by comparing changes in photosynthetic and photoprotective pigments and chlorophyll a fluorescence in foliar tissue with and without galls in naturally growing pedunculate oak trees (Quercus robur L.). The presence of galls of both Cynipidae species caused a significant decrease of chlorophyll a, b and carotenoids contents. Moreover, photosynthetic parameters (F0, Fm, Fv/Fm, Y, qP, qN) were significantly decreased. These results provide valuable information for diagnosing the oak infections using a noninvasive method, such as chlorophyll a fluorescence and predicting the effect of infections on photosynthetic productivity.
The effect of conditioning of Rosa ‘Red Berlin’ stems in solutions of polyamines: spermidine at a concentration of 1.5 and 3 mmol dm⁻³, spermine at a concentration of 1 and 2 mmol dm⁻³ as well as putrescine at 2 and 4 mmol dm⁻³, on their postharvest quality were investigated. Advancing senescence of the stems of Rosa ‘Red Berlin’ resulted in their reduced post-harvest quality, which is evidenced by changes in the values of the plant physiological parameters. Conditioning of the rose stems in the solution of polyamines did not significantly affected the vase life. The most stabilising effect on cytoplasmic membranes of leaf cells had spermine at a concentration of 1 mmol dm⁻³. But the same polyamine applied at a twice higher concentration caused a decrease in the value of EL in petals. The highest values of leaf relative water content were found after conditioning of the rose stems in the solution of putrescine at a concentration of 2 mmol dm⁻³. The highest contents of photosynthetic pigments were noted after the application of spermidine at both concentrations. Conditioning of the rose stems in the solutions of each polyamine increased anthocyanin content in petals and had effect of the values on all analysing physiological parameters in leaves and petals.
The experiment determined the effect of gibberellic acid applied prior to harvest on the contents of plant pigments in cut leaves of wild ginger (Asarum europaeum L.), cultivated in an unheated plastic tunnel and in the field. Foliar application of GA3 at a concentration of 100, 200, 400, 600 mg x dm-3 was repeated four times every two weeks. It has been proven that pre-harvest spraying of plants with gibberellic acid at a concentration of 100 mg x dm-3 has a positive effect on the content of photosynthetically active pigments in the leaves of A. europaeum cultivated in an unheated plastic tunnel. Application of GA3 at a concentration of 600 mg x dm-3 led to the accumulation of the greatest amount of anthocyanins in the leaves of Asarum europaeum cultivated both in the unheated plastic tunnel and in the field. The response of plants to GA3 application, expressed in the amount of flavonoids, depended on conditions related to the cultivation site. Pre-harvest treatment of A. europaeum plants with gibberellic acid at concentrations of 100-600 mg x dm-3 reduced the production of flavonoids in tunnel-grown wild ginger, but enhanced their accumulation in plants cultivated in the field. Pre-harvest application of gibberellic acid did not affect the fresh weight or dry mass content in plant material.
In addition to flowers, cut foliage and leafy stems are very valuable florist material. However, florist greens frequently first loses its ornamental value in a flower arrangement, since it quickly wilts, fades, or browns. That is why it is important to develop agents for conditioning cut the florist greens that would extend its vase life effectively and inhibit senescence. Here we report on the effect of growth regulators and commercially available conditioning products on the post-harvest longevity of cut stems of Weigela florida 'Variegata Nana' and certain processes associated with their senescence. Senescence of W. florida cut stems resulted in reduced post-harvest quality, decrease in relative water content (RWC) and an increase in electrolyte leakage (EL) in the leaf tissues. Chrysal Clear 2, applied in the form of a 24-hour pre-treatment, was the most effective in extending the longevity of cut stems of W. florida. The same conditioning product also had a beneficial effect on leaf tissue water content and the values of the chlorophyll fluorescence parameters, including maximum quantum yield and actual photochemical activity. Conditioning of W. florida stems in a solution of gibberellic acid at a concentration of 0.25 mg · dm-3 had the most beneficial effect on the cytoplasmatic membranes. However, the highest contentes of photosynthetic pigments (chlorophyll “a” and “b”), were found after the 24-hour pre-treatment of W. florida stems in a solution of 0.1 mmol · dm-3 benzyladenine and Chrysal Clear 2.
The effect of Chrysal Glory, GA3-200 mg·dm-3, GA3-400 mg·dm-3, BA-100 mg·dm-3, BA-200 mg·dm-3 on senescence of Paeonia lactiflora 'Profesor Wóycicki' leaves was investigated. Photosynthesis activity of leaves, contents of a and b chlorophyll and leakage of electrolytes from the tissue were estimated. Chrysal Glory suppressed leakage of electrolytes. BA-100 m·dm-3 increased maximum quantum efficiency of chlorophyll. BA-100 mg·dm-3 and GA3-200 mg·dm-3 was the most effective in delaying senescence of Paeonia leaves by retarding chlorophyll loss and suppressig leakage of electrolytes.
The influence of light emitted by light-emitting diodes (LEDs) of different spectral composition towards white fluorescent light FL (W) on the photosynthetic activity of leaves and yield of lettuce was evaluated in pot experiments conducted in controlled conditions at PPFD of 200 µmol m -2 s -1 . The LEDs emitting white (12W), red (12R) and red-blue (R/B) radiation from lamps of different ratio of red diodes (R) towards the blue ones (B) (9R+3B; 10R+2B; 11R+1B) was used. The results showed that the lowest yield was found in plants grown under LED (12W), and the highest one under FL (W) light. The mass and leaf area of plants illuminated by FL (W) and LED (12R) were similar. The increase of radiation R and decrease of B caused an increase in biomass, leaf area (LA), and specific leaf area (SLA) decrease of chlorophyll concentrations in leaves. Leaves of plants cultivated under LED (R/B) had the higher stomatal conductance, photosynthesis and transpiration parameters than under other treatments. The lowest value of chlorophyll fluorescence parameters (Fo, Fm, Fv/Fm) were noted under the LED (12R) lighting. However, taking into account the energy consumption by the using light sources, the plant yielding, and other determined parameters, the most beneficial for lettuce production seems to be LED (11R + 1B) light. Taking into account the energy consumption of light sources, the plant yielding, and other determined in the study parameters, LED (11R + 1B) light appears the most beneficial for lettuce production.
A trumpet creeper (Campsis radicans) is a very decorative shrub propagated vegetatively through cuttings. So far, there is no available information on micropropagation of this beautiful species. Determination of the optimal sterilization methods as well as types and concentrations of plant growth regulators as medium constituents is one of the most important factors of successful micropropagation. With the aim of optimization of in vitro initiation and multiplication of C. radicans, the effect of different methods of disinfection and terms of explants isolation on contamination rate of cultures as well as the influence of cytokinins on growth and branching of shoots was studied. The cytokinins used in the experiments were: benzyladenine (BA), isopentenyl adenine (2-iP) and kinetin (KIN). The obtained results show that contamination rate is a very significant problem to overcome in order to initiate tissue cultures of C. radicans. The best results were observed when explants were excised in spring (May), shortly after the vegetation had started (88% contamination rate). Soaking initial the fragments in a mixture solution of Topsin M 500SC and streptomycine for 12 hours decreased the contamination rate of explants from 100 to 94%. The shoot tips are more suitable to establish the tissue culture of a trumpet creeper, in comparison to nodes with axillary buds. The multiplication rate after two subcultures was 2.6–3.7 for shoot tips (depending on the media) and 1.9–2.1 for nodes. The cytokinins used in the experiment had a significant influence on multiplication rate of C. radicans. The highest number of good quality shoots was obtained on the media supplemented with KIN in concentration of 2 mg˙dm-3
The physiological response of Phalaenopsis × hybridum ‘Innocence’ to biotic stress caused by Pseudococcus longispinus feeding was investigated. The condition of the cytoplasmic membranes expressed by a value of electrolyte outflow (EL) and TBARS and the activity of antioxidative system enzymes: catalase and peroxidase, and the amount of non-enzymatic antioxidant – proline, were determined. The changes in all the analyzed physiological parameters depended on the duration of the pest feeding. The outflow of electrolytes, TBARS content and catalase activity was the highest in the first period of the experiment (after 24-hour of mealybug feeding). Significant increase of peroxidase activity and proline content was noted after 7 days of insects feeding. The values of all analyzed parameters (except EL) demonstrated a decreasing tendency after 14 days of P. longispinus feeding. The observed reaction of P. hybridum ‘Innocence’ testifies to mechanisms triggered with the aim of neutralizing the effects of biotic stress and enabling the normal functioning of the cells in the orchid plants colonized by longtailed mealybug.
The aim of the undertaken research was to establish the subsequent effect of silicon, which is the component in Actisil Hydro Plus preparation, and the place of cultivation on the postharvest quality of Solomon’s Seal (Polygonatum multiflorum ‘Variegatum’). Plants were cultivated directly in the field or in an unheated foil tunnel. The preparation was used as a sixfold spray in three concentrations: 0.2, 0.3 and 0.4% during the plants vegetation, at weekly intervals. The control were plants sprayed with distilled water. Leafy shoots were cut at the moment of becoming morphologically mature and placed in distilled water in a controlled thermal-lightning conditions: temperature was 21/18ºC (day/night) and photoperiod of 12 h light/12 h darkness. The condition of cytoplasmic membrane was evaluated with the use of analysis of electrolyte leakage and determining the level of peroxidation of membrane lipids. The analyses of assimilation pigments (chlorophyll a + b) and proline content, the relative water content as well as postharvest longevity were established. Leafy shoots of P. multiflorum cultivated in an unheated tunnel, regardless of the spray with Actisil Hydro Plus, characterized with longer lasting decorative value. The lowest proline content in tissues after 30 days were observed in leaves obtained from plants cultivated in foil tunnel and sprayed with Actisil Hydro Plus in concentration of 0.2 and 0.3%. During the analysis conducted 30 days after starting the experiment, the most effective stop of membrane lipids peroxidation was observed in shoots obtained from plants cultivated in foil tunnel and sprayed with Actisil in concentration of 0.2%. Cultivation of P. multiflorum in foil tunnel and spray with preparation in concentration of 0.3% inhibited assimilation pigments degradation the most effectively.
Garden perennials are a source of floristry material produced in field or under covers. A perennial producing long, leafy shoots, which in flower arrangements might successfully substitute fern or palm leaves, is Polygonatum multiflorum. The variety characterizing with decorative white margined leaves is ‘Variegatum’. To improve their quality, the growth stimulator Actisil Hydro Plus, which contains 0.6% of active silicon in a form of orthosilicic acid was applied. The aim of the conducted experiment was to estimate the influence of silicon and a place of cultivation (tunnel and open field) on post-harvest quality determined with morphological features, vase life longevity and mechanical properties of Polygonatum multiflorum (L.) All. ‘Variegatum’ stems. The plants were sprayed with water solutions of Actisil in concentrations: 0.2%, 0.3%, and 0.4%, six times during the growing season in weekly intervals, starting from the first decade of May, always in the morning. It was stated that cultivation of Polygonatum in the unheated foil tunnel increases quality of shoots for cut greenery, determined with morphological features and post-harvest longevity. Cultivation of Polygonatum in a foil tunnel and spraying plants with Actisil in concentrations of 0.3 or 0.4% is advantageous due to possibility to increase a number of shoots from 20% to over 31%, their length from 9% to 11% and fresh weight from 52% to 71%, in comparison to control plants. It is advised to treat plants cultivated in a tunnel, with Actisil in concentration of 0.4% as it improved shoots quality evaluated with their diameter and lower leaves blade area. Spraying plants with Actisil in concentrations of 0.2–0.4% in the tunnel and 0.2% in field prolongs post-harvest shoots longevity by 3–4 days. Plants cultivated in the tunnel characterize with higher strength of shoots determined with higher maximum force causing permanent damage and lower stem deflection than plants cultivated in field. Plants grown in a tunnel and sprayed with Actisil in concentrations of 0.4% characterized with the strongest shoots after 14 days of storage, however a similar effect was observed with the concentration of 0.3% and in case of plants cultivated in field and sprayed with 0.4% of Actisil.
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