Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 12

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Bariery rozwojowe polskich przedsiebiorstw handlowych

100%
Nizinska K.
Handel Wewnętrzny
|
1997
|
tom 43
|
nr 3
31-34
2

Źródła przewagi konkurencyjnej przedsiębiorstw oraz sposoby jej osiągania i utrzymywania (część I)

100%
Nizinska K.
Handel Wewnętrzny
|
1995
|
tom 41
|
nr 2
3

Źródła przewagi konkurencyjnej przedsiębiorstw oraz sposoby jej osiągania i utrzymywania (część II)

100%
Nizinska K.
Handel Wewnętrzny
|
1995
|
tom 41
|
nr 3
4

Mikrootoczenie rynkowe przedsiębiorstwa a problemy konkurencji (charakterystyka zależności)

100%
Nizinska K.
Handel Wewnętrzny
|
1995
|
tom 41
|
nr 1
5

Czynniki osiagania sukcesu rynkowego wedlug siedmiu wybranych kanadyjskich przedsiebiorstw handlowo-produkcyjnych

100%
Nizinska K.
Handel Wewnętrzny
|
1994
|
tom 40
|
nr 5-6
58-62
6

Postawy i zachowania konkurencyjne przedsiebiorstw handlowych [czesc 1]

63%
Struzycki M., Nizinska K.
Handel Wewnętrzny
|
1993
|
tom 39
|
nr 6
18-21
7

Postawy i zachowania konkurencyjne przedsiębiorstw handlowych

63%
Struzycki M., Nizinska K.
Handel Wewnętrzny
|
1994
|
tom 40
|
nr 1
8

Behavioral characteristic as a biomarker of development and phenotype of epilepsy in the temporal lobe epilepsy induced by electrical stimulation of the amygdala in rats

51%
Nizinska K., Szydlowska K., Lukasiuk K.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Non-invasive biomarkers of epileptogenesis and epilepsy in experimental models would allow fast and easy evaluation of disease development and impact of treatments aiming at modification of disease development or progress. AIM(S): The project was conducted to test the hypothesis that there are differences in the results of behavioral tests depending on 1) the time to develop epilepsy, measured by the length of the latency to the first spontaneous seizure, and 2) the intensity of epilepsy, measured by the number of seizures. METHOD(S): Animals were kept in enriched environment and were subjected to the handling procedure. Epilepsy was induced by status epilepticus using electrical stimulation of the amygdala (25 min, 100-ms train of 1-ms biphasic square‑wave pluses, 400 µA, 60 Hz, delivered every 0.5 s). EEG was used to classify rats into animals groups with short (<20 days, n=7) and long (>20 days, n=8) latency and into groups with low (SE_L: 62±64.5, n=7) and high (SE_H: 456±185, n=8) seizure number. We applied following behavioral tests: behavioral hyperexcitability, open field, novel object exploration, elevated plus maze and Morris water maze. RESULTS: We observed decreased learning abilities of epileptic animals compared to control (SE: 14.07±9.5, CTRL: 28.36±21.5, p<0.001) in 4 training day of Morris water maze. We observed no difference in the stress level, activity and learning between groups with short and long latency in all behavioral tests and between groups with low and high seizures number. However, we observed increased stress level, measured by number of imputes to the closed arms, in group with high number of seizures in elevated plus maze carried out in 26 week compared to animals with low seizures number (SE_H: 35±13.6, SE_L: 15.14±8.9, p<0.01). CONCLUSIONS: Increased stress level in elevated plus maze might become convenient biomarker of epilepsy phenotype. FINANCIAL SUPPORT: This work was supported by the FP7-HEALTH project 602102 (EPITARGET) and Polish Ministry of Science and Education grant W19/7.PR/2014.
9

Impact of environmental enrichment on anxiety and learning in the rat model of epilepsy induced by electrical stimulation of the amygdala

51%
Stepniak A., Nizinska K., Szydlowska K., Lukasiuk K.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
INTRODUCTION: The environment plays an influential role in the development of many brain disorders; however, its role in modulation of the epilepsy phenotype has not been studied in detail. AIM(S): The aim of this study was to investigate whether environmental enrichment impacts anxiety and learning in an experimental model of epilepsy. METHOD(S): Male Sprague-Dawley rats were allocated to either environmentally enriched (EE; n=13) or standard housing conditions (SH; n=13). Epilepsy was induced by SE (Status epilepticus) evoked by electrical stimulation of the amygdala (25 min, 100 ms train of 1 ms, 60 Hz bipolar pulses, 400 μA, every 0.5 s). The following tests were conducted to assess the behavior of animals: behavioral hyperexcitability, open field, new object recognition, elevated plus maze, social interactions, and the Morris water maze. Blood was withdrawn on days 7 and 29 after stimulation and on the day of perfusion, to assess cortisol levels. RESULTS: Environmental enrichment significantly reduced anxiety levels. We observed reduced mobility in the open field test, a decrease in total distance traveled in the social interactions test, and decreased touch-response in the behavioral hyperexcitability test. SH animals showed impaired spatial memory and learning as compared to EE animals. Rats from the EE group spent more time near a platform in the Morris Water Maze test. Moreover, in RODA analysis, EE control animals showed a trend towards lower thigmotaxis compared to SH animals starting from the 2nd day trial 2, with a significant difference obtained in the 3rd day. Blood analysis demonstrated that SH rats had a significantly higher level of cortisol compared to EE rats. CONCLUSIONS: The present study indicates that environmental enrichment had beneficial effects on anxiety and learning and memory, which may be caused by lower stress hormone levels. FINANCIAL SUPPORT: This work was supported by the FP7‑HEALTH project 602102 (EPITARGET) and Polish Ministry of Science and Education grant W19/7. PR/2014.
10

Circulating microrna as a biomarker of epileptogenesis and epilepsy in the rat model of temporal lobe epilepsy

51%
Szydlowska K., Nizinska K., Bot A., Lukasiuk K.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Epilepsy frequently develops as a result of brain insult, for example: brain injury, status epilepticus, or stroke, however currently there are no tools allowing us to predict which patients suffering from trauma will eventually develop epilepsy or how severe it is going to be. In recent years small non-coding RNAs are proposed as biomarkers for neurological diseases. Particularly microRNAs are interesting candidates, as several of them were described changing their levels in the brain of epileptic subjects. There is evidence suggesting that microRNAs levels are altered also in the plasma, making them attractive candidates for peripheral biomarkers of epilepsy. AIM(S): This study was conducted to evaluate usefulness of plasma miRNAs as biomarkers of epileptogenesis and epilepsy. METHOD(S): In our studies we used the rat model of temporal lobe epilepsy. The status epilepticus was evoked by the stimulation of left lateral nucleus of amygdala. Animals were continuously video and EEG monitored for 6 months. Blood was collected at 14, 30, 60, and 90 days after stimulation from tail vein. Blood plasma was separated and processed using Affymetrix miRNA 4.1 array strip microarrays. RESULTS: We have compared miRNA levels between sham operated (n=12) and stimulated animals (n=15); p<0.01 was used as a cut off. We have detected 14 miRNA differentiating between sham operated and stimulated animals at 14 days, 6 at 30 d, 16 at 60d, and 11 at 90 days. We have also compared the miRNAs levels between animals with high (30–70 seizures/day) and low (1–5 seizures/day) number of seizures. We found differences in levels of 11 miRNA at 14 d, 7 at 30 d, 11 at 60 d and 8 at 90 d (at p<0.01). CONCLUSIONS: Levels of miRNA in plasma are altered during epileptogenesis and differentiate between animals with frequent and rare seizures. miRNA may become a useful biomarker of epileptogenesis/epilepsy as well as severity of the disease. FINANCIAL SUPPORT: This work was supported by the FP7-HEALTH project 602102 (EPITARGET) and Polish Ministry of Science and Education grant W19/7.PR/2014.
11

Impact of environmental enrichment on anxiety and learning in the rat model of epilepsy induced by electrical stimulation of the amygdala

51%
Stepniak A., Nizinska K., Lukasiuk K., Szydlowska K.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Environment plays influential role in the development of many brain disorders, however its role in modulation of epilepsy phenotype has not been studied in details. AIM(S): The aim of this study was to investigate whether environmental enrichment impacts anxiety and learning in experimental model of epilepsy. METHOD(S): Male Sprague-Dawley rats were allocated to either environmentally enriched (EE; n=13) or standard housing condition (SH; n=13). Epilepsy was induced by SE (Status epilepticus) evoked by electrical stimulation of the amygdala (25 min, 100 ms train of 1 ms, 60 Hz bipolar pulses, 400 μA, every 0.5 s). Following tests were conducted to assess the behavior of animals: behavioral hyperexcitability, open field, new object recognition, elevated plus maze, social interactions, and Morris water maze. Blood was withdrawn on days 7 and 29 after stimulation and on the day of perfusion to assess cortisol levels. RESULTS: Environmental enrichment significantly reduced anxiety levels. We observed, reduced mobility in the open field test (EE=2.6±3.3; SH=179.1±107.8 s; p<0,0001), decrease in total distance travelled in the social interactions test (EE=1210.2±574.4; SH=2937.0±711.3 cm; p<0,0001) or decreased touch-response test in the behavioral hyperexcitability test (score: EE=2.1±1.1; SH=3.6±1.8; p<0,0001). SH animals showed impaired spatial memory and learning compared to EE animals. Rats from EE group spent more time near platform (EE=25.5±4.7; SH=21.5±5.0 s; p<0,05) in Morris Water Maze test. Moreover, SH rats showed hyperactivity and thigmotaxis. Blood analysis demonstrated that SH rats had significantly higher level of cortisol (EE=0.4±0.7; SH=1.1±0.6 µg/dl; p<0,01) compared to EE rats. CONCLUSIONS: The present study indicates that environmental enrichment had beneficial effects on anxiety and learning and memory, which may be caused by lower stress hormone levels. FINANCIAL SUPPORT: This work was supported by the FP7-HEALTH project 602102 (EPITARGET) and Polish Ministry of Science and Education grant W19/7. PR/2014.
12

Methyl‑CpG binding domain 3 promoter activity in a rat model of seizure evoked by intraperitional injection of pentylenetetrazol (PTZ)

51%
Nizinska K., Stepniak A., Lukasiuk K.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
INTRODUCTION: Animal models for seizures and epilepsy have played a fundamental role in advancing our understanding of basic mechanisms underlying epileptogenesis and epilepsy. During epileptogenesis and epilepsy, several molecular and cellular changes occur, including alterations in gene and protein expression. MBD3 (Methyl-CpG binding domain 3) protein is a reader of DNA methylation marks, which changed its expression in epileptogenesis. AIM(S): The aim of this study was to determine changes in MBD3 protein expression after acute seizure in the rat brain. METHOD(S): Spraque‑Dawley rats were kept in an enriched environment and were subjected to handling procedure. A single interperitional injection of pentylenetetrazol (PTZ, 40 mg/kg) was used to evoke tonic‑clonic seizure. Control rats (n=16) which were injected by saline and rats after PTZ administration (n=16) were observed for an hour after injection. To examine changes in RNA expression and protein level, animals were sacrificed at selected time points: 1 h, 4 h, 8 h and 24 h after injection. Changes in MBD3 protein levels were examined in the hippocampus, entorhinal, and somatosensory cortex using Western Blot with anti-MBD3 antibody (#A302-528A, Bethyl) followed by ImageJ analysis, whereas changes on RNA level were examined with Real Time PCR. RESULTS: No significant differences were observed in RNA levels in the hippocampus, entorhinal, and somatosensory cortex during 24 h after injection. Western Blot analysis showed an increased level of MBD3 protein at 4 h after seizures evoked by PTZ injection in the somatosensory cortex. PTZ did not affect MBD3 protein expression in the hippocampus and entorhinal cortex at 4 h, 8 h, and 24 h after injection, nor in the somatosensory cortex at 8 h and 24 h after PTZ injection. CONCLUSIONS: These results showed that seizures influence MBD3 protein expression and therefore MBD3 may play an important role in epileptogenesis or epilepsy. FINANCIAL SUPPORT: This work is supported by the Polish Ministry of Science and Education grant 2015/19/B/NZ4/01401.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.