The study determined optimum conditions for ultrafiltration of an acid-rennet whey. Experiments were made with the deproteinized ultrafiltrate toward its utilization in the production of the yeast-bacterial biomass. To this end, growth dynamics of Kluyveromyces fragilis yeast and Propionibacterium shermanii were determined by using different propagation times. It was shown utilization of such combined cultures renders biomasses rich in quality protein and vitamins (mainly vit. B₁₂) Initial yeasting of a culture prior to introduction of propionic bacteria makes it possible to obtain higher yields of biomass and vit. B₁₂ within a period shorter than the traditional cultures of propionic bacteria require. The project was also concerned with analyzing the composition of the protein concentrates obtained by ultrafiltration. It was indicated that these concentrates should be mixed with the yeast-bacterial biomasses obtained from the same whey.