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This paper attempts to give a microeconomic explanation of the environmental Kuznets curve (EKC) hypothesis according to marginal cost and marginal revenue. The interpretation about the shape and inflection point of EKC is based on the comparison between marginal cost and marginal revenue. The emissions of carbon dioxide (CO₂), nitrous oxide (N₂O) and methane are used as the environmental indicators and GDP per person is used as the economic indicator. Through the analysis of U.S. data from 1960 to 2010, we investigated the EKC hypothesis using the co-integration and correlation methods. The curve of CO₂ versus GDP per person in the U.S. is a wave shape in a different time scale. The relationship between N₂O and GDP per person showed the same trend with CO₂. The EKC curve between the methane and the GDP per person from 1990 to 2009 is a U-shape. Thus, we analyzed the relationship according to the microeconomic explanation of the EKC.
Twenty days’ exposure to 50 or 100 mM NaCl in the rooting medium substantially increased fresh and dry weights of seedling shoots of the recretohalophyte Limonium sinense while 200 or 300 mM were increasingly inhibitory. KCl treatment was only slightly stimulating (50 mM) or strongly inhibitory (100–300 mM). Lesser effects on leaf area were also seen. Diameter of foliar salt glands was significantly larger than that of controls in 100 and 200 mM NaCl with the effect being reversed at higher concentrations. Gland enlargement was also observed in the presence of 100 mM KCl, while larger concentrations reduced gland size. Generally, gland diameter was larger in the presence of NaCl than in KCl. NaCl and KCl also increased gland number per leaf and secretion rate per gland. At 100 and 200 mM NaCl or KCl, Na⁺ secretion per leaf from NaCl-treated plants exceeded K⁺ secretion rate from KCl-treated plants while at 200 mM, Na⁺ secretion per gland was significantly higher for Na⁺ than for K⁺. Evidence of cell death in leaves of salt-treated plants using Evans blue staining indicates that release of cell contents through loss of membrane integrity contributed to the secretion values. We conclude that the greater tolerance of L. sinenseto to NaCl compared to KCl is linked to the more effective secretion of Na⁺ than of K⁺ and, in turn, to a greater stimulation of salt gland formation and activity and larger gland diameter.
Suaeda salsa calli treated with different concentrations of NaCl were used to examine the response of the plasma membrane (PM) H⁺-ATPase to NaCl and its role in salt tolerance. The optimum concentration of NaCl for growth of the calli was 50 mM, while growth was significantly inhibited at 250 mM NaCl. The ion and organic solute contents of calli increased with increasing NaCl. Activity of the PM H⁺-ATPase increased when the calli were treated with NaCl over a certain concentration range (0–150 mM NaCl). However, the activity reached its maximum with 150 mM NaCl. Immunoblotting analysis of the PM H⁺-ATPase protein from calli cultures with anti- Zea mays H⁺-ATPase serum (monoclonal 46E5B11D5) identified a single polypeptide of ~90 kDa. The peptide levels increased in the calli treated with NaCl at 150 mM NaCl compared to control, but the increase at 50 mM NaCl was less pronounced. Northern blot analysis showed that the expression of the PM H⁺-ATPase also increased after the calli were treated with NaCl. These results suggest that the increase in PM H⁺-ATPase activity is due to both an increase in the amount of PM H⁺-ATPase protein and an up-regulation of the PM H⁺-ATPase gene, which is involved in the salt tolerance of S. salsa calli.
Medicinal Salvia miltiorrhiza is renowned for its curative effects on cardiovascular diseases. Its biologically active ingredients include rosmarinic acid (RA) and its derivative, salvianolic acid B (SAB). We used available bioinformatics tools to improve our knowledge about the biosynthesis of these phenolic compounds. Our comprehensive description of cis-acting regulatory elements in the RA pathway provides insights into the potential transcriptional regulation of that pathway. For example, a lightresponsive element was the most abundant and widespread motif, suggesting that light is a universal regulatory factor of RA synthesis in S. miltiorrhiza. Therefore, we examined gene transcripts and the accumulation of hydrophilic pharmacological compounds in light-treated plants. Canonical correlation analysis was also used to construct a gene-tometabolite network. We obtained a high correlation coefficient (0.986), which generally indicated a clear and close relationship between RA-biosynthetic genes and desirable metabolites. We also screened PAL1, C4H, and HPPR, genes directly linked to the accumulation of RA and SAB. Our results can serve as the basis for better understanding RA synthesis in S. miltiorrhiza, and they will increase the practical potential for metabolic engineering of this important medicinal species.
Auxin has been widely implicated in various aspects of plant growth and development, including flower development. In order to further elucidate the role of auxin during flower development, especially on the pistil development process, auxin response factors (ARFs), an important component in auxin signalling pathway, were studied in the early flower buds of Japanese apricot (Prunus mume Sieb. et Zucc). In this study, a comprehensive overview of the ARF gene family in Japanese apricot is presented, including the chromosomal locations, phylogenetic relationships, gene structures, the domain and nuclear localization analysis. Seventeen Japanese apricot genes that encode ARF proteins (PmARFs) have been identified based on the genome sequence of Japanese apricot. Comparison of the expression of some PmARF genes between perfect and imperfect flower buds in Japanese apricot suggests that PmARFs, especially the PmARF13 and PmARF17 gene may be required for pistil development and function in Japanese apricot. These results will be useful for future functional analyses of the ARF family genes in plants.
Suaeda salsa L., a C3 euhalophytic herb, is native to saline soils, demonstrates high resistance to salinity stress. The effect of chilling stress on S. salsa under high salinity, particularly the change in unsaturated fatty acid content within membrane lipids, has not been investigated. After a 12 h chilling treatment (4°C) performed under low irradiance (100 μmol m⁻² s⁻¹), the chlorophyll contents, maximal photochemical efficiency of photosystem II (Fv/Fm) and actual PSII efficiency (ΦPSII) were determined. These measurements were significantly decreased in S. salsa leaves in the absence of salt treatment yet there were no significant changes with a 200 mM NaCl treatment. Chlorophyll contents, Fv/Fm and ΦPSII in S. salsa under 200 mM NaCl were higher than those without salt treatment. The unsaturated fatty acid content and the double bond index (DBI) of major membrane lipids of monogalactosyldiacylglycerols, digalactosyldiacylglycerols (DGDG), sulphoquinovosyldiacylglycerols and phosphatidylglycerols (PG) significantly increased following the chilling treatment (4°C) (with 12 h of low irradiance and 200 mM of NaCl). The DBI of DGDG and PG was decreased in the absence of the salt treatment. These results suggest that in the euhalophyte S. salsa, a 200 mM NaCl treatment increases chilling tolerance under conditions of low irradiance (100 μmol m⁻² s⁻¹).
Thellungiella halophila is a salt tolerant relative of Arabidopsis thaliana with high genetic and morphological similarity. In the present study, effects of salinity on germination and seedling growth of T. halophila and A. thaliana were compared. The present results showed that the salinity inhibited seed germination in both species. Unexpectedly, percentages of seed germination in A. thaliana were higher than T. halophila in a range of 0–200 mM NaCl. Seeds of both species could not germinate when the concentration of NaCl was over 200 mM. However, when compared with A. thaliana, seeds of T. halophila did not suffer ion toxicity, as evidenced by the higher final germination rate after ungerminated seeds pretreated with NaCl were transferred to distilled water. Seedlings of T. halophila were more salt tolerant than those of A. thaliana, e.g., seedlings of T. halophila had better plant growth (root length, fresh and dry mass), higher chlorophyll content, less MDA content and higher proline content and K⁺/Na⁺ ratio under salinity. These results indicate that T. halophila is more salt tolerant than A. thaliana during both seed germination and seedling stages and explain why A. thaliana is excluded from saline locations and T. halophila can survive in saline soils.
Post-anthesis high temperature stress is a major concern for the winter wheat producing areas in east China, especially in Shandong province. Fourteen agronomic and physiological traits were studied in 58 Chinese wheat accessions under high-temperature conditions in 2012 and 2013. High temperature stress led to reduction in grain yield and other yield component traits. Significant variations were observed for grain yield per plant (GYPP), grain weight per spike (GWS), thousand kernel weight (TKW), grain number of the main-spike, spikelet number and biological yield per plant. Among the physiological traits measured, chlorophyll content and normalized difference vegetation index showed significant variation under high temperature stress. The results indicate that heat susceptibility index estimated for TKW, GYPP, GWS and flag leaf senescence scale (FLSS) could be used as selection criteria for identifying heat tolerant genotypes for the Shandong province. The results also suggest that FLSS is a good, quick visual indicator of staygreen for field selection for heat tolerance. After harvest, TKW can be measured as final indicator of heat tolerance. The varieties Gaoyou 9415, Hemai 13, Jimai 22, Kexin 9, Shannong 8355, Taishan 23, Yannong 5286 and Zimai 7 were heat tolerant and potential germplasm for heat tolerance breeding in Shandong province and other parts of China. The results could be useful for breeding climate resilient varieties for China or other countries in the world suffering high temperature stresses during grain filling period.
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