Wyniki wyszukiwania

1

A novel method of Mycobacterium tuberculosis complex strain differentiation using polymorphic GC-rich gene sequences

100%

Kotlowski R.

Acta Biochimica Polonica

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2015

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tom 62

|

nr 2

2

Gorace wedzenie ryb w lagodnych warunkach

63%

Sikorski Z. E., Kotlowski R.

Przemysł Spożywczy

|

1998

|

tom 52

|

nr 07

34-36

Gorące wędzenie nie zawsze zapewnia zniszczenie C. botulinum i L. monocytogenes w rybach, gdyż niektórzy producenci stosują bardzo łagodne warunki temperatury i czasu oraz male stężenie soli, chcąc uzyskać dobrą sensoryczną jakość produktu. Aby wykluczyć niebezpieczeństwo zatruć mikrobiologicznych i zakażeń pokarmowych, można stosować łagodne warunki wędzenia tylko wtedy, jeśli w przetwórni stosuje się zasady HACCP, a towar przechowuje się nieprzerwanie w temp. poniżej 3°C.

3

Identification of evolutionary conserved DNA sequence and corresponding S21 ribosomal protein region for diagnostic purposes of all Borrelia spirochetes

63%

Kotlowski R., Holec-Gasior L.

Acta Biochimica Polonica

|

2019

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tom 66

|

nr 1

4

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A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

63%

Woloszyn A., Kotlowski R.

Roczniki Państwowego Zakładu Higieny

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2017

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tom 68

|

nr 3

Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic peptides - amanitins and phallotoxins present in the most dangerous poisonous mushrooms. Material and Methods. Degenerate primers in the PCR were designed on the basis of amanitins (n=13) and phallotoxins (n=5) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information. Results. The specificity of the PCR assays was confirmed against 9 species of edible mushrooms, death cap - Amanita phalloides and panther cap - Amanita pantherina. Conclusions. Designed two couples of PCR-primers specific to amanitins and phallotoxins genes can be recommended for detection of Amanita phalloides and other mushroom species producing hepatotoxic cyclic peptides - amanitins and phallotoxins.

5

Lizostafyna - zrodla otrzymywania i zastosowanie

51%

Szweda P., Kotlowski R., Kur J.

Biotechnologia

|

2005

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nr 4

28-45

6

Differentiation of Listeria monocytogenes strains within the iap gene

51%

Kotlowski R., Jura M., Alsalami A. A., Szweda P.

Cellular and Molecular Biology Letters

|

2001

|

tom 06

|

nr 3A

802

7

Zastosowanie metody PCR do wykrywania Listeria monocytogenes w mleku

51%

Wieckowska M., Kotlowski R., Kur J., Rudnicka W.

Medycyna Doświadczalna i Mikrobiologia

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1998

|

tom 50

|

nr 3-4

251-257

Przeprowadzono elektroforetyczne i serologiczne badania porównawcze składu białek 6 szczepów M. pneumoniae i 1 szczepu M. genitalium. Stwierdzono pełne podobieństwo budowy antygenowej badanych szczepów M. pneumoniae oraz bliskie podobieństwo antygenowe M. genitalium do M. pneumoniae. Najbardziej swoistymi spośród antygenów M. pneumoniae okazały się białka o masie cząsteczkowej 170 (białko P1) i 89 kDa, uważane za najważniejsze adhezyny komórki mykoplazmowej.

8

Detection and differentiation of E. coli serotypes carrying EAE gene

51%

Kotlowski R., Ziomek M., Hernik A., Szweda P.

Cellular and Molecular Biology Letters

|

2001

|

tom 06

|

nr 3A

826

9

Differentiation of Listeria monocytogenes on the basis of hemolytic and phosphatidylinositol specific phospholipase C activity and by PCR method

51%

Kotlowski R., Kaczmarek M., Kur J., Rudnicka W.

Polish Journal of Food and Nutrition Sciences

|

1996

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tom 05

|

nr 3

The possibility of differentiation of L.monocytogenes from other Listeria species on the basis of hemolytic activity, the production of phosphatidylinositol-specific phospholipase C (PI-PLC) and the polymerase chain reaction (PCR) for the amplification of a DNA fragment of listeriolysine O (hly A) gene was compared. The screening of Listeria colonies for PI-PLC activity allowed to distinguish the pathogenic for humans L.monocytogenes bacteria from the majority of non-pathogenic Listeria spp. The amplification of DNA from Listeria lysates with two primers selected in area of the hly A gene made possible the differentiation of L.monocytogenes from other Listeria species, including hemolytic L.ivanovii and L.seeligeri bacteria as well as hemolytic or PI-PLC positive L.innocua strains.

10

Listeria monocytogenes in salted herring

51%

Dabrowski W., Rozycka-Kasztelan K., Kur J., Kotlowski R.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2000

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tom 03

|

nr 2

The following paper includes the results of research, concerning the occurrence of Listeria spp in salted herring and herring salads. 100 samples of traditionally and vacuum packed herring and 40 herring salads were examined. It was established that 6.6% of the samples were contaminated with these microorganisms. 4 strains of Listeria innocua and 5 strains of L. monocytogenes were isolated. Listeria was not found in the herring salads, which was explained by the low, <4 pH of the product. Moreover, the ability of Listeria monocytogenes to develop in the environment of salted herring was determined. It was established that the pathogenic microorganisms multiplies in the herring, stored at the room temperature. At 10°C the development of Listeria is totally inhibited. Sodium benzoate has little influence on the development of Listeria because it merely delays the beginning, of the logarithmic phase of the bacteria development by 2 days.

11

Amplification of a single-locus variable-number direct repeats with restriction fragment length polymorphism [DR-PCR-RFLP] for genetic typing of Acinetobacter baumannii strains

45%

Nowak-Zaleska A., Krawczyk B., Kotlowski R., Mikucka A., Gospodarek E.

Polish Journal of Microbiology

|

2008

|

tom 57

|

nr 1

In search of an effective DNA typing techniaue for Acinetobacter baumannii strains for hospital epidemiology use, the performance and convenience of a new target seauence was evaluated. Using known genomic seauences of Acinetobacter baumannii strains AR 319754 and ATCC 17978, we developed single-locus variable-number direct-repeat analysis using polymerase chain reaction-restriction fragment length polymorphism (DR-PCR/RFLP) method. A total of 90 Acinetobacter baumannii strains isolated from patients of the Clinical Hospital in Bydgoszcz, Poland, were examined. Initially, all strains were typed using macrorestriction analysis of the chromosomal DNA by pulsed-field gel electrophoresis (REA-PFGE). Digestion of the chromosomal DNA with the Apal endonuclease and separation of the fragments by PFGE revealed 21 uniaue types. Application of DR-PCR/RFLP resulted in recognition of 12 clusters. The results showed that the DR-PCR/RFLP method is less discriminatory than REA-PFGE, however, the novel genotyping method can be used as an alternative techniaue for generating DNA profiles in epidemiological studies of intra-species genetic relatedness of Acinetobacter baumannii strains.

Ograniczanie wyników

A novel method of Mycobacterium tuberculosis complex strain differentiation using polymorphic GC-rich gene sequences

Gorace wedzenie ryb w lagodnych warunkach

Identification of evolutionary conserved DNA sequence and corresponding S21 ribosomal protein region for diagnostic purposes of all Borrelia spirochetes

A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

Lizostafyna - zrodla otrzymywania i zastosowanie

Differentiation of Listeria monocytogenes strains within the iap gene

Zastosowanie metody PCR do wykrywania Listeria monocytogenes w mleku

Detection and differentiation of E. coli serotypes carrying EAE gene

Differentiation of Listeria monocytogenes on the basis of hemolytic and phosphatidylinositol specific phospholipase C activity and by PCR method

Listeria monocytogenes in salted herring

Amplification of a single-locus variable-number direct repeats with restriction fragment length polymorphism [DR-PCR-RFLP] for genetic typing of Acinetobacter baumannii strains